Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0441 and BT0442 are separated by 17 nucleotides BT0442 and BT0443 are separated by 225 nucleotides BT0443 and BT0444 are separated by 11 nucleotides
BT0441: BT0441 - hypothetical protein (NCBI ptt file), at 535,032 to 536,420
BT0441
BT0442: BT0442 - glycerophosphoryl diester phosphodiesterase (NCBI ptt file), at 536,438 to 538,213
BT0442
BT0443: BT0443 - thermostable beta-glucosidase B (NCBI ptt file), at 538,439 to 538,756
BT0443
BT0444: BT0444 - hypothetical protein (NCBI ptt file), at 538,768 to 540,024
BT0444
Position (kb) 536
537
538
539 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 535.519 kb on + strand, within BT0441 at 535.519 kb on + strand, within BT0441 at 535.526 kb on + strand, within BT0441 at 535.702 kb on - strand, within BT0441 at 535.704 kb on - strand, within BT0441 at 535.775 kb on - strand, within BT0441 at 535.849 kb on - strand, within BT0441 at 535.863 kb on + strand, within BT0441 at 535.863 kb on + strand, within BT0441 at 535.864 kb on - strand, within BT0441 at 535.868 kb on - strand, within BT0441 at 535.883 kb on + strand, within BT0441 at 535.884 kb on - strand, within BT0441 at 535.904 kb on + strand, within BT0441 at 535.907 kb on - strand, within BT0441 at 535.941 kb on - strand, within BT0441 at 535.985 kb on - strand, within BT0441 at 536.146 kb on - strand, within BT0441 at 536.358 kb on - strand at 536.371 kb on - strand at 536.422 kb on + strand at 536.461 kb on - strand at 536.491 kb on + strand at 536.492 kb on - strand at 536.492 kb on - strand at 536.498 kb on - strand at 536.498 kb on - strand at 536.520 kb on + strand at 536.524 kb on + strand at 536.567 kb on + strand at 536.618 kb on + strand, within BT0442 at 536.721 kb on - strand, within BT0442 at 536.764 kb on - strand, within BT0442 at 536.865 kb on + strand, within BT0442 at 536.865 kb on + strand, within BT0442 at 536.869 kb on - strand, within BT0442 at 536.869 kb on - strand, within BT0442 at 536.985 kb on + strand, within BT0442 at 537.091 kb on + strand, within BT0442 at 537.092 kb on - strand, within BT0442 at 537.188 kb on + strand, within BT0442 at 537.189 kb on - strand, within BT0442 at 537.348 kb on - strand, within BT0442 at 537.348 kb on - strand, within BT0442 at 537.349 kb on + strand, within BT0442 at 537.399 kb on - strand, within BT0442 at 537.432 kb on - strand, within BT0442 at 537.433 kb on + strand, within BT0442 at 537.434 kb on - strand, within BT0442 at 537.613 kb on - strand, within BT0442 at 537.640 kb on - strand, within BT0442 at 537.696 kb on - strand, within BT0442 at 537.742 kb on - strand, within BT0442 at 537.915 kb on - strand, within BT0442 at 537.949 kb on + strand, within BT0442 at 538.043 kb on + strand at 538.044 kb on - strand at 538.058 kb on + strand at 538.063 kb on + strand at 538.230 kb on - strand at 538.260 kb on + strand at 538.264 kb on + strand at 538.267 kb on - strand at 538.366 kb on + strand at 538.403 kb on + strand at 538.459 kb on - strand at 538.470 kb on + strand at 538.534 kb on - strand, within BT0443 at 538.700 kb on - strand, within BT0443 at 538.761 kb on - strand at 538.761 kb on - strand at 538.802 kb on + strand at 538.948 kb on - strand, within BT0444 at 538.949 kb on + strand, within BT0444 at 538.961 kb on + strand, within BT0444 at 539.002 kb on - strand, within BT0444 at 539.026 kb on + strand, within BT0444 at 539.110 kb on + strand, within BT0444 at 539.173 kb on + strand, within BT0444 at 539.173 kb on + strand, within BT0444
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 535,519 + BT0441 0.35 -0.7 535,519 + BT0441 0.35 -3.3 535,526 + BT0441 0.36 -1.3 535,702 - BT0441 0.48 -0.2 535,704 - BT0441 0.48 -2.3 535,775 - BT0441 0.53 -0.8 535,849 - BT0441 0.59 +2.9 535,863 + BT0441 0.60 +1.3 535,863 + BT0441 0.60 +0.7 535,864 - BT0441 0.60 -1.3 535,868 - BT0441 0.60 -1.3 535,883 + BT0441 0.61 -1.9 535,884 - BT0441 0.61 -2.9 535,904 + BT0441 0.63 -1.3 535,907 - BT0441 0.63 -1.7 535,941 - BT0441 0.65 +1.3 535,985 - BT0441 0.69 -2.3 536,146 - BT0441 0.80 +2.0 536,358 - -1.9 536,371 - -3.1 536,422 + +0.7 536,461 - -1.3 536,491 + +0.3 536,492 - +2.6 536,492 - -1.3 536,498 - +1.9 536,498 - +1.0 536,520 + -0.1 536,524 + +1.5 536,567 + -2.3 536,618 + BT0442 0.10 -2.6 536,721 - BT0442 0.16 -1.4 536,764 - BT0442 0.18 +0.7 536,865 + BT0442 0.24 -0.3 536,865 + BT0442 0.24 -0.5 536,869 - BT0442 0.24 -4.5 536,869 - BT0442 0.24 -2.8 536,985 + BT0442 0.31 +1.3 537,091 + BT0442 0.37 -1.9 537,092 - BT0442 0.37 +0.7 537,188 + BT0442 0.42 -5.0 537,189 - BT0442 0.42 +0.4 537,348 - BT0442 0.51 +2.4 537,348 - BT0442 0.51 -2.6 537,349 + BT0442 0.51 -2.5 537,399 - BT0442 0.54 -3.5 537,432 - BT0442 0.56 -2.6 537,433 + BT0442 0.56 -0.3 537,434 - BT0442 0.56 -1.9 537,613 - BT0442 0.66 -1.3 537,640 - BT0442 0.68 -2.8 537,696 - BT0442 0.71 -0.8 537,742 - BT0442 0.73 +0.4 537,915 - BT0442 0.83 -2.3 537,949 + BT0442 0.85 -1.3 538,043 + +0.5 538,044 - -2.7 538,058 + -3.7 538,063 + -1.3 538,230 - +0.3 538,260 + -4.6 538,264 + +0.7 538,267 - +2.0 538,366 + +1.5 538,403 + -2.7 538,459 - -0.5 538,470 + -0.9 538,534 - BT0443 0.30 +0.8 538,700 - BT0443 0.82 +1.2 538,761 - +0.9 538,761 - +1.2 538,802 + +0.5 538,948 - BT0444 0.14 +0.5 538,949 + BT0444 0.14 +0.3 538,961 + BT0444 0.15 +1.3 539,002 - BT0444 0.19 -2.6 539,026 + BT0444 0.21 +0.3 539,110 + BT0444 0.27 -2.7 539,173 + BT0444 0.32 +1.5 539,173 + BT0444 0.32 -2.9
Or see this region's nucleotide sequence