Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0428 and BT0429 are separated by 11 nucleotides BT0429 and BT0430 are separated by 3 nucleotides
BT0428: BT0428 - phenylacetate-coenzyme A ligase (NCBI ptt file), at 514,550 to 515,857
BT0428
BT0429: BT0429 - indolepyruvate oxidoreductase subunit iorB (NCBI ptt file), at 515,869 to 516,453
BT0429
BT0430: BT0430 - indolepyruvate oxidoreductase subunit iorA (NCBI ptt file), at 516,457 to 518,049
BT0430
Position (kb) 515
516
517 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 514.935 kb on - strand, within BT0428 at 514.994 kb on - strand, within BT0428 at 515.067 kb on + strand, within BT0428 at 515.153 kb on - strand, within BT0428 at 515.156 kb on - strand, within BT0428 at 515.158 kb on - strand, within BT0428 at 515.158 kb on - strand, within BT0428 at 515.261 kb on - strand, within BT0428 at 515.298 kb on + strand, within BT0428 at 515.315 kb on + strand, within BT0428 at 515.330 kb on - strand, within BT0428 at 515.363 kb on - strand, within BT0428 at 515.558 kb on - strand, within BT0428 at 515.594 kb on + strand, within BT0428 at 515.702 kb on - strand, within BT0428 at 515.702 kb on - strand, within BT0428 at 515.705 kb on - strand, within BT0428 at 515.746 kb on - strand at 515.866 kb on + strand at 515.867 kb on - strand at 515.870 kb on + strand at 515.917 kb on - strand at 516.091 kb on - strand, within BT0429 at 516.118 kb on + strand, within BT0429 at 516.207 kb on - strand, within BT0429 at 516.360 kb on - strand, within BT0429 at 516.391 kb on - strand, within BT0429 at 516.437 kb on - strand at 516.439 kb on - strand at 516.463 kb on - strand at 516.924 kb on + strand, within BT0430 at 516.935 kb on + strand, within BT0430 at 516.968 kb on + strand, within BT0430 at 516.969 kb on - strand, within BT0430 at 516.973 kb on - strand, within BT0430 at 516.973 kb on - strand, within BT0430 at 517.087 kb on + strand, within BT0430 at 517.106 kb on - strand, within BT0430 at 517.151 kb on + strand, within BT0430 at 517.363 kb on + strand, within BT0430 at 517.367 kb on + strand, within BT0430 at 517.368 kb on - strand, within BT0430
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 514,935 - BT0428 0.29 +3.6 514,994 - BT0428 0.34 +0.4 515,067 + BT0428 0.40 -3.1 515,153 - BT0428 0.46 +0.4 515,156 - BT0428 0.46 -4.8 515,158 - BT0428 0.46 +1.5 515,158 - BT0428 0.46 +1.3 515,261 - BT0428 0.54 +0.6 515,298 + BT0428 0.57 -1.9 515,315 + BT0428 0.58 -4.1 515,330 - BT0428 0.60 -2.3 515,363 - BT0428 0.62 -0.8 515,558 - BT0428 0.77 +0.7 515,594 + BT0428 0.80 -1.9 515,702 - BT0428 0.88 -0.3 515,702 - BT0428 0.88 +0.7 515,705 - BT0428 0.88 -0.3 515,746 - -2.3 515,866 + -2.3 515,867 - -0.6 515,870 + -2.6 515,917 - +1.4 516,091 - BT0429 0.38 +1.0 516,118 + BT0429 0.43 -0.3 516,207 - BT0429 0.58 +3.3 516,360 - BT0429 0.84 +0.9 516,391 - BT0429 0.89 +5.0 516,437 - -1.2 516,439 - +1.7 516,463 - +4.0 516,924 + BT0430 0.29 -1.3 516,935 + BT0430 0.30 -6.2 516,968 + BT0430 0.32 -3.1 516,969 - BT0430 0.32 -0.3 516,973 - BT0430 0.32 -0.1 516,973 - BT0430 0.32 -0.6 517,087 + BT0430 0.40 -0.3 517,106 - BT0430 0.41 -1.6 517,151 + BT0430 0.44 -4.4 517,363 + BT0430 0.57 -0.3 517,367 + BT0430 0.57 -0.3 517,368 - BT0430 0.57 +0.7
Or see this region's nucleotide sequence