Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0325 and BT0326 overlap by 4 nucleotides BT0326 and BT0327 are separated by 442 nucleotides BT0327 and BT0328 are separated by 17 nucleotides
BT0325: BT0325 - hypothetical protein (NCBI ptt file), at 391,028 to 391,489
BT0325
BT0326: BT0326 - putative RNA polymerase ECF-type sigma factor (NCBI ptt file), at 391,486 to 392,007
BT0326
BT0327: BT0327 - glutamine ABC transporter, periplasmic glutamine-binding protein (glnH) (NCBI ptt file), at 392,450 to 393,289
BT0327
BT0328: BT0328 - hypothetical protein (NCBI ptt file), at 393,307 to 395,349
BT0328
Position (kb) 392
393
394 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 391.800 kb on + strand, within BT0326 at 391.984 kb on + strand at 392.168 kb on + strand at 392.196 kb on + strand at 392.219 kb on - strand at 392.224 kb on - strand at 392.231 kb on + strand at 392.232 kb on - strand at 392.235 kb on + strand at 392.363 kb on - strand at 392.363 kb on - strand at 392.363 kb on - strand at 392.403 kb on - strand at 392.464 kb on - strand at 392.464 kb on - strand at 392.522 kb on - strand at 392.603 kb on - strand, within BT0327 at 392.734 kb on + strand, within BT0327 at 392.735 kb on - strand, within BT0327 at 392.894 kb on + strand, within BT0327 at 393.070 kb on - strand, within BT0327 at 393.118 kb on + strand, within BT0327 at 393.121 kb on - strand, within BT0327 at 393.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 391,800 + BT0326 0.60 -2.3 391,984 + -0.6 392,168 + +1.3 392,196 + -1.0 392,219 - -0.6 392,224 - -1.5 392,231 + +3.4 392,232 - +0.9 392,235 + -2.3 392,363 - +1.3 392,363 - -0.6 392,363 - -0.9 392,403 - -0.2 392,464 - -0.3 392,464 - +0.1 392,522 - -1.3 392,603 - BT0327 0.18 +3.3 392,734 + BT0327 0.34 +3.3 392,735 - BT0327 0.34 +2.0 392,894 + BT0327 0.53 -0.3 393,070 - BT0327 0.74 -0.3 393,118 + BT0327 0.80 +2.0 393,121 - BT0327 0.80 +1.3 393,234 - -0.7
Or see this region's nucleotide sequence