Experiment: BHIS with Trimethoprim 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0003 and BT0004 are separated by 134 nucleotides BT0004 and BT0005 are separated by 8 nucleotides BT0005 and BT0006 are separated by 91 nucleotides
BT0003: BT0003 - hypothetical protein (NCBI ptt file), at 1,872 to 2,648
BT0003
BT0004: BT0004 - hypothetical protein (NCBI ptt file), at 2,783 to 3,451
BT0004
BT0005: BT0005 - 30S ribosomal protein S17 (NCBI ptt file), at 3,460 to 3,996
BT0005
BT0006: BT0006 - hypothetical protein (NCBI ptt file), at 4,088 to 4,534
BT0006
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1.854 kb on + strand at 1.981 kb on + strand, within BT0003 at 1.982 kb on - strand, within BT0003 at 1.982 kb on - strand, within BT0003 at 2.048 kb on - strand, within BT0003 at 2.049 kb on + strand, within BT0003 at 2.138 kb on - strand, within BT0003 at 2.176 kb on - strand, within BT0003 at 2.263 kb on - strand, within BT0003 at 2.263 kb on - strand, within BT0003 at 2.267 kb on - strand, within BT0003 at 2.382 kb on - strand, within BT0003 at 2.430 kb on - strand, within BT0003 at 2.483 kb on - strand, within BT0003 at 2.521 kb on - strand, within BT0003 at 2.528 kb on + strand, within BT0003 at 2.534 kb on + strand, within BT0003 at 2.535 kb on - strand, within BT0003 at 2.535 kb on - strand, within BT0003 at 2.556 kb on - strand, within BT0003 at 2.573 kb on + strand at 2.697 kb on - strand at 2.697 kb on - strand at 2.697 kb on - strand at 2.707 kb on - strand at 2.777 kb on - strand at 2.797 kb on - strand at 2.813 kb on - strand at 2.955 kb on - strand, within BT0004 at 2.955 kb on - strand, within BT0004 at 3.192 kb on + strand, within BT0004 at 3.232 kb on + strand, within BT0004 at 3.422 kb on - strand at 3.426 kb on - strand at 3.458 kb on - strand at 3.550 kb on - strand, within BT0005 at 3.557 kb on + strand, within BT0005 at 3.638 kb on - strand, within BT0005 at 3.694 kb on - strand, within BT0005 at 3.696 kb on + strand, within BT0005 at 3.697 kb on - strand, within BT0005 at 3.746 kb on + strand, within BT0005 at 3.747 kb on - strand, within BT0005 at 3.747 kb on - strand, within BT0005 at 3.785 kb on + strand, within BT0005 at 3.872 kb on - strand, within BT0005 at 3.917 kb on + strand, within BT0005 at 3.918 kb on - strand, within BT0005 at 3.918 kb on - strand, within BT0005 at 4.104 kb on + strand at 4.208 kb on - strand, within BT0006 at 4.258 kb on - strand, within BT0006 at 4.278 kb on - strand, within BT0006 at 4.278 kb on - strand, within BT0006 at 4.280 kb on - strand, within BT0006 at 4.280 kb on - strand, within BT0006 at 4.291 kb on - strand, within BT0006 at 4.301 kb on - strand, within BT0006 at 4.304 kb on + strand, within BT0006 at 4.324 kb on + strand, within BT0006 at 4.335 kb on - strand, within BT0006 at 4.364 kb on - strand, within BT0006 at 4.367 kb on + strand, within BT0006
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.0125 mM remove 1,854 + +0.5 1,981 + BT0003 0.14 -2.2 1,982 - BT0003 0.14 +2.5 1,982 - BT0003 0.14 -1.2 2,048 - BT0003 0.23 -0.2 2,049 + BT0003 0.23 -2.2 2,138 - BT0003 0.34 +3.2 2,176 - BT0003 0.39 -0.2 2,263 - BT0003 0.50 +3.4 2,263 - BT0003 0.50 -2.2 2,267 - BT0003 0.51 +0.4 2,382 - BT0003 0.66 +3.5 2,430 - BT0003 0.72 -1.9 2,483 - BT0003 0.79 +0.5 2,521 - BT0003 0.84 +0.8 2,528 + BT0003 0.84 -2.8 2,534 + BT0003 0.85 -2.2 2,535 - BT0003 0.85 +1.8 2,535 - BT0003 0.85 -4.9 2,556 - BT0003 0.88 +0.9 2,573 + -2.1 2,697 - -1.4 2,697 - -2.0 2,697 - -2.5 2,707 - -0.2 2,777 - -1.4 2,797 - -1.1 2,813 - -0.5 2,955 - BT0004 0.26 -0.2 2,955 - BT0004 0.26 -2.8 3,192 + BT0004 0.61 -2.2 3,232 + BT0004 0.67 -0.8 3,422 - -0.2 3,426 - -1.2 3,458 - -3.4 3,550 - BT0005 0.17 -2.0 3,557 + BT0005 0.18 -5.7 3,638 - BT0005 0.33 -2.8 3,694 - BT0005 0.44 -1.6 3,696 + BT0005 0.44 -1.5 3,697 - BT0005 0.44 -2.5 3,746 + BT0005 0.53 -2.2 3,747 - BT0005 0.53 +1.5 3,747 - BT0005 0.53 +4.7 3,785 + BT0005 0.61 -0.6 3,872 - BT0005 0.77 +3.2 3,917 + BT0005 0.85 -0.2 3,918 - BT0005 0.85 -1.4 3,918 - BT0005 0.85 -2.2 4,104 + -3.2 4,208 - BT0006 0.27 -4.0 4,258 - BT0006 0.38 -0.8 4,278 - BT0006 0.43 +1.2 4,278 - BT0006 0.43 -2.8 4,280 - BT0006 0.43 +0.9 4,280 - BT0006 0.43 -1.5 4,291 - BT0006 0.45 -0.2 4,301 - BT0006 0.48 -1.2 4,304 + BT0006 0.48 -1.2 4,324 + BT0006 0.53 -4.1 4,335 - BT0006 0.55 -1.8 4,364 - BT0006 0.62 +1.1 4,367 + BT0006 0.62 -2.2
Or see this region's nucleotide sequence