Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00770 and BWI76_RS00775 are separated by 241 nucleotides BWI76_RS00775 and BWI76_RS00780 are separated by 94 nucleotides BWI76_RS00780 and BWI76_RS00785 are separated by 266 nucleotides
BWI76_RS00770: BWI76_RS00770 - primosomal protein N', at 156,482 to 158,677
_RS00770
BWI76_RS00775: BWI76_RS00775 - 50S ribosomal protein L31, at 158,919 to 159,134
_RS00775
BWI76_RS00780: BWI76_RS00780 - met repressor, at 159,229 to 159,546
_RS00780
BWI76_RS00785: BWI76_RS00785 - cystathionine gamma-synthase, at 159,813 to 160,973
_RS00785
Position (kb) 159
160 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 159.493 kb on + strand, within BWI76_RS00780 at 159.623 kb on - strand at 159.654 kb on + strand at 159.654 kb on + strand at 159.803 kb on - strand at 159.889 kb on + strand at 160.023 kb on + strand, within BWI76_RS00785 at 160.223 kb on + strand, within BWI76_RS00785 at 160.262 kb on + strand, within BWI76_RS00785 at 160.277 kb on - strand, within BWI76_RS00785 at 160.337 kb on - strand, within BWI76_RS00785 at 160.423 kb on + strand at 160.526 kb on - strand, within BWI76_RS00785 at 160.546 kb on + strand, within BWI76_RS00785
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 159,493 + BWI76_RS00780 0.83 -0.5 159,623 - +0.1 159,654 + -0.1 159,654 + -0.6 159,803 - -1.8 159,889 + -4.7 160,023 + BWI76_RS00785 0.18 -3.8 160,223 + BWI76_RS00785 0.35 -2.4 160,262 + BWI76_RS00785 0.39 -2.6 160,277 - BWI76_RS00785 0.40 -2.5 160,337 - BWI76_RS00785 0.45 -3.2 160,423 + +3.3 160,526 - BWI76_RS00785 0.61 -3.3 160,546 + BWI76_RS00785 0.63 -4.0
Or see this region's nucleotide sequence