Experiment: MRS + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16735 and BBR_RS16740 are separated by 132 nucleotides BBR_RS16740 and BBR_RS16745 are separated by 12 nucleotides BBR_RS16745 and BBR_RS16750 overlap by 1 nucleotides
BBR_RS16735: BBR_RS16735 - NUDIX hydrolase, at 1,527,534 to 1,528,370
_RS16735
BBR_RS16740: BBR_RS16740 - SMC-Scp complex subunit ScpB, at 1,528,503 to 1,529,177
_RS16740
BBR_RS16745: BBR_RS16745 - segregation/condensation protein A, at 1,529,190 to 1,530,041
_RS16745
BBR_RS16750: BBR_RS16750 - ParA family protein, at 1,530,041 to 1,530,880
_RS16750
Position (kb)
1529
1530
1531 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1528.229 kb on - strand, within BBR_RS16735 at 1528.245 kb on - strand, within BBR_RS16735 at 1528.294 kb on - strand at 1528.294 kb on - strand at 1528.300 kb on + strand at 1528.314 kb on + strand at 1528.314 kb on - strand at 1528.325 kb on - strand at 1528.331 kb on + strand at 1528.331 kb on + strand at 1528.339 kb on - strand at 1528.339 kb on - strand at 1528.339 kb on - strand at 1528.339 kb on - strand at 1528.339 kb on - strand at 1528.389 kb on + strand at 1528.565 kb on - strand at 1528.604 kb on - strand, within BBR_RS16740 at 1528.604 kb on - strand, within BBR_RS16740 at 1528.671 kb on - strand, within BBR_RS16740 at 1528.687 kb on + strand, within BBR_RS16740 at 1528.752 kb on + strand, within BBR_RS16740 at 1528.753 kb on - strand, within BBR_RS16740 at 1528.788 kb on + strand, within BBR_RS16740 at 1528.801 kb on + strand, within BBR_RS16740 at 1528.804 kb on - strand, within BBR_RS16740 at 1528.820 kb on + strand, within BBR_RS16740 at 1528.869 kb on + strand, within BBR_RS16740 at 1528.899 kb on - strand, within BBR_RS16740 at 1528.901 kb on + strand, within BBR_RS16740 at 1528.909 kb on - strand, within BBR_RS16740 at 1528.909 kb on - strand, within BBR_RS16740 at 1528.937 kb on - strand, within BBR_RS16740 at 1528.937 kb on - strand, within BBR_RS16740 at 1528.937 kb on - strand, within BBR_RS16740 at 1528.947 kb on - strand, within BBR_RS16740 at 1528.990 kb on + strand, within BBR_RS16740 at 1528.995 kb on - strand, within BBR_RS16740 at 1528.995 kb on - strand, within BBR_RS16740 at 1529.003 kb on - strand, within BBR_RS16740 at 1529.007 kb on + strand, within BBR_RS16740 at 1529.007 kb on + strand, within BBR_RS16740 at 1529.013 kb on + strand, within BBR_RS16740 at 1529.016 kb on - strand, within BBR_RS16740 at 1529.023 kb on + strand, within BBR_RS16740 at 1529.023 kb on - strand, within BBR_RS16740 at 1529.023 kb on - strand, within BBR_RS16740 at 1529.050 kb on - strand, within BBR_RS16740 at 1529.050 kb on - strand, within BBR_RS16740 at 1529.095 kb on + strand, within BBR_RS16740 at 1529.108 kb on - strand, within BBR_RS16740 at 1529.129 kb on + strand at 1529.129 kb on + strand at 1529.139 kb on - strand at 1529.326 kb on + strand, within BBR_RS16745 at 1529.326 kb on + strand, within BBR_RS16745 at 1529.334 kb on - strand, within BBR_RS16745 at 1529.334 kb on - strand, within BBR_RS16745 at 1529.334 kb on - strand, within BBR_RS16745 at 1529.378 kb on + strand, within BBR_RS16745 at 1529.386 kb on - strand, within BBR_RS16745 at 1529.433 kb on - strand, within BBR_RS16745 at 1529.556 kb on - strand, within BBR_RS16745 at 1529.574 kb on - strand, within BBR_RS16745 at 1529.594 kb on + strand, within BBR_RS16745 at 1529.597 kb on - strand, within BBR_RS16745 at 1529.679 kb on + strand, within BBR_RS16745 at 1529.708 kb on - strand, within BBR_RS16745 at 1529.731 kb on - strand, within BBR_RS16745 at 1529.831 kb on - strand, within BBR_RS16745 at 1529.954 kb on + strand, within BBR_RS16745 at 1529.954 kb on + strand, within BBR_RS16745 at 1529.954 kb on + strand, within BBR_RS16745 at 1529.962 kb on - strand at 1529.962 kb on - strand at 1529.962 kb on - strand at 1529.991 kb on + strand at 1530.010 kb on + strand at 1530.300 kb on + strand, within BBR_RS16750 at 1530.322 kb on + strand, within BBR_RS16750 at 1530.339 kb on - strand, within BBR_RS16750 at 1530.339 kb on - strand, within BBR_RS16750 at 1530.355 kb on + strand, within BBR_RS16750 at 1530.374 kb on + strand, within BBR_RS16750 at 1530.382 kb on - strand, within BBR_RS16750 at 1530.478 kb on - strand, within BBR_RS16750 at 1530.478 kb on - strand, within BBR_RS16750 at 1530.506 kb on - strand, within BBR_RS16750 at 1530.676 kb on - strand, within BBR_RS16750 at 1530.745 kb on + strand, within BBR_RS16750 at 1530.767 kb on + strand, within BBR_RS16750 at 1530.783 kb on + strand, within BBR_RS16750 at 1530.853 kb on - strand at 1530.989 kb on + strand at 1530.989 kb on + strand at 1531.026 kb on + strand at 1531.034 kb on - strand at 1531.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time48hr remove 1,528,229 - BBR_RS16735 0.83 -1.6 1,528,245 - BBR_RS16735 0.85 +1.7 1,528,294 - -0.2 1,528,294 - -2.9 1,528,300 + +0.4 1,528,314 + -2.2 1,528,314 - -2.7 1,528,325 - -1.0 1,528,331 + -0.6 1,528,331 + -0.6 1,528,339 - -0.6 1,528,339 - -1.8 1,528,339 - +0.2 1,528,339 - -0.2 1,528,339 - +1.2 1,528,389 + +0.4 1,528,565 - +1.0 1,528,604 - BBR_RS16740 0.15 +1.0 1,528,604 - BBR_RS16740 0.15 +2.5 1,528,671 - BBR_RS16740 0.25 +1.8 1,528,687 + BBR_RS16740 0.27 +0.0 1,528,752 + BBR_RS16740 0.37 -0.3 1,528,753 - BBR_RS16740 0.37 -1.2 1,528,788 + BBR_RS16740 0.42 -1.9 1,528,801 + BBR_RS16740 0.44 +1.0 1,528,804 - BBR_RS16740 0.45 +1.4 1,528,820 + BBR_RS16740 0.47 -1.6 1,528,869 + BBR_RS16740 0.54 -0.1 1,528,899 - BBR_RS16740 0.59 -1.5 1,528,901 + BBR_RS16740 0.59 +1.3 1,528,909 - BBR_RS16740 0.60 +0.4 1,528,909 - BBR_RS16740 0.60 +2.0 1,528,937 - BBR_RS16740 0.64 +2.5 1,528,937 - BBR_RS16740 0.64 -4.1 1,528,937 - BBR_RS16740 0.64 -0.2 1,528,947 - BBR_RS16740 0.66 -0.3 1,528,990 + BBR_RS16740 0.72 +0.2 1,528,995 - BBR_RS16740 0.73 +1.5 1,528,995 - BBR_RS16740 0.73 -0.3 1,529,003 - BBR_RS16740 0.74 +0.4 1,529,007 + BBR_RS16740 0.75 -0.6 1,529,007 + BBR_RS16740 0.75 +0.4 1,529,013 + BBR_RS16740 0.76 +1.0 1,529,016 - BBR_RS16740 0.76 +0.5 1,529,023 + BBR_RS16740 0.77 -0.6 1,529,023 - BBR_RS16740 0.77 -1.4 1,529,023 - BBR_RS16740 0.77 +0.4 1,529,050 - BBR_RS16740 0.81 -0.6 1,529,050 - BBR_RS16740 0.81 +1.0 1,529,095 + BBR_RS16740 0.88 -3.1 1,529,108 - BBR_RS16740 0.90 +0.7 1,529,129 + -2.9 1,529,129 + +0.3 1,529,139 - +1.5 1,529,326 + BBR_RS16745 0.16 +1.0 1,529,326 + BBR_RS16745 0.16 +1.5 1,529,334 - BBR_RS16745 0.17 -3.1 1,529,334 - BBR_RS16745 0.17 +0.4 1,529,334 - BBR_RS16745 0.17 +0.4 1,529,378 + BBR_RS16745 0.22 +0.4 1,529,386 - BBR_RS16745 0.23 -1.0 1,529,433 - BBR_RS16745 0.29 -0.3 1,529,556 - BBR_RS16745 0.43 +3.1 1,529,574 - BBR_RS16745 0.45 -1.0 1,529,594 + BBR_RS16745 0.47 -1.6 1,529,597 - BBR_RS16745 0.48 +0.2 1,529,679 + BBR_RS16745 0.57 +2.1 1,529,708 - BBR_RS16745 0.61 -1.7 1,529,731 - BBR_RS16745 0.63 -0.9 1,529,831 - BBR_RS16745 0.75 -1.1 1,529,954 + BBR_RS16745 0.90 -3.0 1,529,954 + BBR_RS16745 0.90 -0.3 1,529,954 + BBR_RS16745 0.90 -2.6 1,529,962 - +0.1 1,529,962 - +1.2 1,529,962 - -0.3 1,529,991 + +0.4 1,530,010 + -0.6 1,530,300 + BBR_RS16750 0.31 -0.6 1,530,322 + BBR_RS16750 0.33 +1.0 1,530,339 - BBR_RS16750 0.35 -2.2 1,530,339 - BBR_RS16750 0.35 -0.0 1,530,355 + BBR_RS16750 0.37 -3.4 1,530,374 + BBR_RS16750 0.40 -2.2 1,530,382 - BBR_RS16750 0.41 -1.6 1,530,478 - BBR_RS16750 0.52 -0.6 1,530,478 - BBR_RS16750 0.52 -0.6 1,530,506 - BBR_RS16750 0.55 -0.6 1,530,676 - BBR_RS16750 0.76 -0.6 1,530,745 + BBR_RS16750 0.84 -3.8 1,530,767 + BBR_RS16750 0.86 -0.6 1,530,783 + BBR_RS16750 0.88 -0.6 1,530,853 - -0.6 1,530,989 + +0.5 1,530,989 + +0.1 1,531,026 + -2.6 1,531,034 - -4.0 1,531,039 - +0.3
Or see this region's nucleotide sequence