Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03010 and BWI76_RS03015 are separated by 164 nucleotides BWI76_RS03015 and BWI76_RS03020 are separated by 52 nucleotides BWI76_RS03020 and BWI76_RS03025 are separated by 99 nucleotides BWI76_RS03025 and BWI76_RS03030 are separated by 123 nucleotides
BWI76_RS03010: BWI76_RS03010 - ornithine carbamoyltransferase, at 615,898 to 616,902
_RS03010
BWI76_RS03015: BWI76_RS03015 - RNase E inhibitor protein, at 617,067 to 617,492
_RS03015
BWI76_RS03020: BWI76_RS03020 - tRNA hydroxylase, at 617,545 to 618,303
_RS03020
BWI76_RS03025: BWI76_RS03025 - N-acetyltransferase, at 618,403 to 618,906
_RS03025
BWI76_RS03030: BWI76_RS03030 - valine--tRNA ligase, at 619,030 to 621,885
_RS03030
Position (kb) 617
618
619 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 616.695 kb on + strand, within BWI76_RS03010 at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.816 kb on - strand at 616.844 kb on - strand at 616.906 kb on - strand at 617.167 kb on - strand, within BWI76_RS03015 at 617.323 kb on + strand, within BWI76_RS03015 at 617.607 kb on - strand at 617.654 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.761 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 618.331 kb on - strand at 618.410 kb on + strand at 618.565 kb on + strand, within BWI76_RS03025 at 618.573 kb on - strand, within BWI76_RS03025 at 618.605 kb on - strand, within BWI76_RS03025 at 618.884 kb on - strand at 618.885 kb on - strand at 618.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 616,695 + BWI76_RS03010 0.79 -3.4 616,808 + -3.1 616,808 + -4.4 616,808 + -2.7 616,808 + -1.9 616,808 + -3.2 616,816 - -3.2 616,844 - -1.9 616,906 - -2.9 617,167 - BWI76_RS03015 0.23 -0.1 617,323 + BWI76_RS03015 0.60 -1.0 617,607 - +0.6 617,654 - BWI76_RS03020 0.14 -0.4 617,665 - BWI76_RS03020 0.16 -0.1 617,665 - BWI76_RS03020 0.16 -0.1 617,761 - BWI76_RS03020 0.28 +0.1 617,995 - BWI76_RS03020 0.59 -0.9 617,995 - BWI76_RS03020 0.59 +0.4 618,331 - -0.7 618,410 + -0.6 618,565 + BWI76_RS03025 0.32 -0.1 618,573 - BWI76_RS03025 0.34 -2.6 618,605 - BWI76_RS03025 0.40 -0.4 618,884 - -1.6 618,885 - +0.5 618,966 + -0.1
Or see this region's nucleotide sequence