Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_29325 and AO356_29330 are separated by 144 nucleotides AO356_29330 and AO356_29335 are separated by 46 nucleotides AO356_29335 and AO356_29340 are separated by 34 nucleotides
AO356_29325: AO356_29325 - TonB-dependent receptor, at 6,820,041 to 6,822,173
_29325
AO356_29330: AO356_29330 - ABC transporter substrate-binding protein, at 6,822,318 to 6,823,295
_29330
AO356_29335: AO356_29335 - sugar dehydrogenase, at 6,823,342 to 6,824,103
_29335
AO356_29340: AO356_29340 - multidrug DMT transporter permease, at 6,824,138 to 6,824,965
_29340
Position (kb)
6822
6823
6824 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6821.323 kb on - strand, within AO356_29325 at 6821.445 kb on + strand, within AO356_29325 at 6821.446 kb on - strand, within AO356_29325 at 6821.451 kb on + strand, within AO356_29325 at 6821.451 kb on + strand, within AO356_29325 at 6821.451 kb on + strand, within AO356_29325 at 6821.611 kb on - strand, within AO356_29325 at 6821.611 kb on - strand, within AO356_29325 at 6821.620 kb on - strand, within AO356_29325 at 6821.698 kb on - strand, within AO356_29325 at 6821.838 kb on + strand, within AO356_29325 at 6821.875 kb on - strand, within AO356_29325 at 6821.875 kb on - strand, within AO356_29325 at 6822.012 kb on + strand at 6822.013 kb on - strand at 6822.121 kb on - strand at 6822.121 kb on - strand at 6822.654 kb on + strand, within AO356_29330 at 6822.654 kb on + strand, within AO356_29330 at 6822.655 kb on - strand, within AO356_29330 at 6822.655 kb on - strand, within AO356_29330 at 6822.655 kb on - strand, within AO356_29330 at 6822.841 kb on - strand, within AO356_29330 at 6822.881 kb on + strand, within AO356_29330 at 6822.978 kb on + strand, within AO356_29330 at 6822.979 kb on - strand, within AO356_29330 at 6822.979 kb on - strand, within AO356_29330 at 6822.979 kb on - strand, within AO356_29330 at 6822.987 kb on + strand, within AO356_29330 at 6822.990 kb on + strand, within AO356_29330 at 6822.991 kb on - strand, within AO356_29330 at 6823.042 kb on + strand, within AO356_29330 at 6823.042 kb on + strand, within AO356_29330 at 6823.044 kb on + strand, within AO356_29330 at 6823.045 kb on - strand, within AO356_29330 at 6823.171 kb on - strand, within AO356_29330 at 6823.186 kb on + strand, within AO356_29330 at 6823.343 kb on + strand at 6823.343 kb on + strand at 6823.344 kb on - strand at 6823.517 kb on + strand, within AO356_29335 at 6823.517 kb on + strand, within AO356_29335 at 6823.517 kb on + strand, within AO356_29335 at 6823.518 kb on - strand, within AO356_29335 at 6823.518 kb on - strand, within AO356_29335 at 6823.518 kb on - strand, within AO356_29335 at 6823.518 kb on - strand, within AO356_29335 at 6823.518 kb on - strand, within AO356_29335 at 6823.518 kb on - strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.521 kb on + strand, within AO356_29335 at 6823.522 kb on - strand, within AO356_29335 at 6823.522 kb on - strand, within AO356_29335 at 6823.522 kb on - strand, within AO356_29335 at 6823.522 kb on - strand, within AO356_29335 at 6823.522 kb on - strand, within AO356_29335 at 6823.629 kb on - strand, within AO356_29335 at 6823.629 kb on - strand, within AO356_29335 at 6823.791 kb on + strand, within AO356_29335 at 6823.991 kb on + strand, within AO356_29335 at 6823.992 kb on - strand, within AO356_29335 at 6823.992 kb on - strand, within AO356_29335 at 6823.992 kb on - strand, within AO356_29335 at 6823.992 kb on - strand, within AO356_29335 at 6823.992 kb on - strand, within AO356_29335
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 6,821,323 - AO356_29325 0.60 +0.5 6,821,445 + AO356_29325 0.66 +0.1 6,821,446 - AO356_29325 0.66 -0.2 6,821,451 + AO356_29325 0.66 -0.2 6,821,451 + AO356_29325 0.66 +0.7 6,821,451 + AO356_29325 0.66 -0.7 6,821,611 - AO356_29325 0.74 +0.7 6,821,611 - AO356_29325 0.74 +1.4 6,821,620 - AO356_29325 0.74 +1.1 6,821,698 - AO356_29325 0.78 +0.5 6,821,838 + AO356_29325 0.84 +1.4 6,821,875 - AO356_29325 0.86 +0.7 6,821,875 - AO356_29325 0.86 -0.3 6,822,012 + +1.2 6,822,013 - +0.1 6,822,121 - -0.2 6,822,121 - -0.5 6,822,654 + AO356_29330 0.34 -0.5 6,822,654 + AO356_29330 0.34 -1.2 6,822,655 - AO356_29330 0.34 -0.4 6,822,655 - AO356_29330 0.34 -0.2 6,822,655 - AO356_29330 0.34 -0.7 6,822,841 - AO356_29330 0.53 +0.7 6,822,881 + AO356_29330 0.58 -0.4 6,822,978 + AO356_29330 0.67 +0.8 6,822,979 - AO356_29330 0.68 +0.6 6,822,979 - AO356_29330 0.68 +0.3 6,822,979 - AO356_29330 0.68 +0.1 6,822,987 + AO356_29330 0.68 -0.2 6,822,990 + AO356_29330 0.69 +0.3 6,822,991 - AO356_29330 0.69 -0.1 6,823,042 + AO356_29330 0.74 -0.2 6,823,042 + AO356_29330 0.74 -0.2 6,823,044 + AO356_29330 0.74 +1.8 6,823,045 - AO356_29330 0.74 -0.2 6,823,171 - AO356_29330 0.87 +0.3 6,823,186 + AO356_29330 0.89 -0.9 6,823,343 + -0.5 6,823,343 + +1.0 6,823,344 - +0.7 6,823,517 + AO356_29335 0.23 -0.8 6,823,517 + AO356_29335 0.23 +0.5 6,823,517 + AO356_29335 0.23 +0.3 6,823,518 - AO356_29335 0.23 +0.1 6,823,518 - AO356_29335 0.23 +0.1 6,823,518 - AO356_29335 0.23 -1.2 6,823,518 - AO356_29335 0.23 +0.2 6,823,518 - AO356_29335 0.23 -0.2 6,823,518 - AO356_29335 0.23 -1.9 6,823,521 + AO356_29335 0.23 -0.5 6,823,521 + AO356_29335 0.23 +1.5 6,823,521 + AO356_29335 0.23 +0.1 6,823,521 + AO356_29335 0.23 +0.4 6,823,521 + AO356_29335 0.23 +0.5 6,823,521 + AO356_29335 0.23 +0.5 6,823,521 + AO356_29335 0.23 +0.0 6,823,521 + AO356_29335 0.23 +0.7 6,823,521 + AO356_29335 0.23 -0.3 6,823,521 + AO356_29335 0.23 -0.0 6,823,522 - AO356_29335 0.24 -0.7 6,823,522 - AO356_29335 0.24 -0.2 6,823,522 - AO356_29335 0.24 -0.2 6,823,522 - AO356_29335 0.24 +0.7 6,823,522 - AO356_29335 0.24 -0.0 6,823,629 - AO356_29335 0.38 +0.5 6,823,629 - AO356_29335 0.38 -0.7 6,823,791 + AO356_29335 0.59 -0.2 6,823,991 + AO356_29335 0.85 +0.3 6,823,992 - AO356_29335 0.85 +0.6 6,823,992 - AO356_29335 0.85 -0.8 6,823,992 - AO356_29335 0.85 +0.4 6,823,992 - AO356_29335 0.85 -0.3 6,823,992 - AO356_29335 0.85 +0.6
Or see this region's nucleotide sequence