Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_26105 and AO356_26110 overlap by 11 nucleotides AO356_26110 and AO356_26115 overlap by 8 nucleotides AO356_26115 and AO356_26120 overlap by 4 nucleotides AO356_26120 and AO356_26125 are separated by 144 nucleotides
AO356_26105: AO356_26105 - FAD-dependent oxidoreductase, at 5,910,823 to 5,911,902
_26105
AO356_26110: AO356_26110 - sarcosine oxidase subunit alpha, at 5,911,892 to 5,912,221
_26110
AO356_26115: AO356_26115 - (2Fe-2S)-binding protein, at 5,912,214 to 5,913,587
_26115
AO356_26120: AO356_26120 - cytochrome C2, at 5,913,584 to 5,913,934
_26120
AO356_26125: AO356_26125 - ABC transporter substrate-binding protein, at 5,914,079 to 5,914,861
_26125
Position (kb) 5912
5913
5914 Strain fitness (log2 ratio) -1
0
1
2 at 5911.522 kb on + strand, within AO356_26105 at 5911.522 kb on + strand, within AO356_26105 at 5911.522 kb on + strand, within AO356_26105 at 5911.522 kb on + strand, within AO356_26105 at 5911.523 kb on - strand, within AO356_26105 at 5911.523 kb on - strand, within AO356_26105 at 5911.523 kb on - strand, within AO356_26105 at 5911.523 kb on - strand, within AO356_26105 at 5911.523 kb on - strand, within AO356_26105 at 5911.523 kb on - strand, within AO356_26105 at 5911.661 kb on + strand, within AO356_26105 at 5911.662 kb on - strand, within AO356_26105 at 5911.720 kb on + strand, within AO356_26105 at 5911.986 kb on + strand, within AO356_26110 at 5912.066 kb on + strand, within AO356_26110 at 5912.066 kb on + strand, within AO356_26110 at 5912.451 kb on + strand, within AO356_26115 at 5912.502 kb on + strand, within AO356_26115 at 5912.503 kb on - strand, within AO356_26115 at 5912.503 kb on - strand, within AO356_26115 at 5912.675 kb on - strand, within AO356_26115 at 5912.701 kb on - strand, within AO356_26115 at 5913.226 kb on - strand, within AO356_26115 at 5913.232 kb on + strand, within AO356_26115 at 5913.233 kb on - strand, within AO356_26115 at 5913.296 kb on + strand, within AO356_26115 at 5913.297 kb on - strand, within AO356_26115 at 5913.504 kb on + strand at 5913.507 kb on + strand at 5913.590 kb on - strand at 5913.590 kb on - strand at 5913.590 kb on - strand at 5913.590 kb on - strand at 5914.055 kb on + strand at 5914.055 kb on + strand at 5914.056 kb on - strand at 5914.238 kb on + strand, within AO356_26125 at 5914.238 kb on + strand, within AO356_26125 at 5914.239 kb on - strand, within AO356_26125 at 5914.450 kb on + strand, within AO356_26125 at 5914.508 kb on - strand, within AO356_26125 at 5914.508 kb on - strand, within AO356_26125 at 5914.520 kb on + strand, within AO356_26125 at 5914.520 kb on + strand, within AO356_26125 at 5914.565 kb on + strand, within AO356_26125
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 5,911,522 + AO356_26105 0.65 +0.2 5,911,522 + AO356_26105 0.65 +1.1 5,911,522 + AO356_26105 0.65 +0.4 5,911,522 + AO356_26105 0.65 -0.3 5,911,523 - AO356_26105 0.65 -0.3 5,911,523 - AO356_26105 0.65 +0.2 5,911,523 - AO356_26105 0.65 +0.5 5,911,523 - AO356_26105 0.65 -0.5 5,911,523 - AO356_26105 0.65 -0.5 5,911,523 - AO356_26105 0.65 +2.5 5,911,661 + AO356_26105 0.78 -0.3 5,911,662 - AO356_26105 0.78 +0.8 5,911,720 + AO356_26105 0.83 -0.3 5,911,986 + AO356_26110 0.28 +0.2 5,912,066 + AO356_26110 0.53 -0.5 5,912,066 + AO356_26110 0.53 +0.2 5,912,451 + AO356_26115 0.17 -0.5 5,912,502 + AO356_26115 0.21 -1.0 5,912,503 - AO356_26115 0.21 +0.7 5,912,503 - AO356_26115 0.21 -0.9 5,912,675 - AO356_26115 0.34 -0.2 5,912,701 - AO356_26115 0.35 -0.5 5,913,226 - AO356_26115 0.74 -0.3 5,913,232 + AO356_26115 0.74 +0.6 5,913,233 - AO356_26115 0.74 +0.7 5,913,296 + AO356_26115 0.79 +0.2 5,913,297 - AO356_26115 0.79 +0.0 5,913,504 + -0.2 5,913,507 + +0.7 5,913,590 - +0.8 5,913,590 - +0.5 5,913,590 - -0.3 5,913,590 - +0.8 5,914,055 + -0.2 5,914,055 + +0.3 5,914,056 - +0.5 5,914,238 + AO356_26125 0.20 +0.9 5,914,238 + AO356_26125 0.20 +1.1 5,914,239 - AO356_26125 0.20 -0.5 5,914,450 + AO356_26125 0.47 +0.1 5,914,508 - AO356_26125 0.55 -0.1 5,914,508 - AO356_26125 0.55 +1.0 5,914,520 + AO356_26125 0.56 +1.8 5,914,520 + AO356_26125 0.56 -0.5 5,914,565 + AO356_26125 0.62 +0.2
Or see this region's nucleotide sequence