Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24735 and AO356_24740 overlap by 4 nucleotides AO356_24740 and AO356_24745 are separated by 37 nucleotides AO356_24745 and AO356_24750 are separated by 227 nucleotides AO356_24750 and AO356_24755 overlap by 4 nucleotides
AO356_24735: AO356_24735 - TonB-dependent outer membrane receptor, at 5,596,167 to 5,596,799
_24735
AO356_24740: AO356_24740 - RNA polymerase subunit sigma-24, at 5,596,796 to 5,597,341
_24740
AO356_24745: AO356_24745 - iron dicitrate transport regulator FecR, at 5,597,379 to 5,598,320
_24745
AO356_24750: AO356_24750 - type II secretion system protein GspJ, at 5,598,548 to 5,599,159
_24750
AO356_24755: AO356_24755 - type II secretion system protein GspH, at 5,599,156 to 5,599,608
_24755
Position (kb) 5597
5598
5599 Strain fitness (log2 ratio) -1
0
1
2 at 5596.401 kb on + strand, within AO356_24735 at 5596.402 kb on - strand, within AO356_24735 at 5596.494 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.532 kb on - strand, within AO356_24735 at 5596.844 kb on + strand at 5596.845 kb on - strand at 5596.920 kb on - strand, within AO356_24740 at 5596.984 kb on + strand, within AO356_24740 at 5597.141 kb on + strand, within AO356_24740 at 5597.142 kb on - strand, within AO356_24740 at 5597.346 kb on - strand at 5597.712 kb on + strand, within AO356_24745 at 5597.712 kb on + strand, within AO356_24745 at 5598.036 kb on + strand, within AO356_24745 at 5598.036 kb on + strand, within AO356_24745 at 5598.492 kb on + strand at 5598.493 kb on - strand at 5598.550 kb on - strand at 5598.577 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 5,596,401 + AO356_24735 0.37 +1.8 5,596,402 - AO356_24735 0.37 +0.4 5,596,494 + AO356_24735 0.52 -0.2 5,596,531 + AO356_24735 0.58 +0.6 5,596,531 + AO356_24735 0.58 +0.7 5,596,531 + AO356_24735 0.58 +1.8 5,596,532 - AO356_24735 0.58 +0.4 5,596,844 + +1.1 5,596,845 - +0.6 5,596,920 - AO356_24740 0.23 -0.2 5,596,984 + AO356_24740 0.34 -0.6 5,597,141 + AO356_24740 0.63 -0.6 5,597,142 - AO356_24740 0.63 -0.6 5,597,346 - -1.2 5,597,712 + AO356_24745 0.35 -0.4 5,597,712 + AO356_24745 0.35 -0.2 5,598,036 + AO356_24745 0.70 +0.6 5,598,036 + AO356_24745 0.70 -0.9 5,598,492 + -0.1 5,598,493 - -0.6 5,598,550 - +0.0 5,598,577 - +0.3
Or see this region's nucleotide sequence