Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23330 and AO356_23335 are separated by 195 nucleotides AO356_23335 and AO356_23340 are separated by 172 nucleotides
AO356_23330: AO356_23330 - aminoglycoside phosphotransferase, at 5,231,429 to 5,232,496
_23330
AO356_23335: AO356_23335 - hypothetical protein, at 5,232,692 to 5,233,087
_23335
AO356_23340: AO356_23340 - chemotaxis protein, at 5,233,260 to 5,235,287
_23340
Position (kb) 5232
5233
5234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5231.991 kb on + strand, within AO356_23330 at 5231.992 kb on - strand, within AO356_23330 at 5231.992 kb on - strand, within AO356_23330 at 5232.033 kb on + strand, within AO356_23330 at 5232.033 kb on + strand, within AO356_23330 at 5232.081 kb on - strand, within AO356_23330 at 5232.165 kb on + strand, within AO356_23330 at 5232.166 kb on - strand, within AO356_23330 at 5232.166 kb on - strand, within AO356_23330 at 5232.207 kb on + strand, within AO356_23330 at 5232.207 kb on + strand, within AO356_23330 at 5232.207 kb on + strand, within AO356_23330 at 5232.207 kb on + strand, within AO356_23330 at 5232.207 kb on + strand, within AO356_23330 at 5232.208 kb on - strand, within AO356_23330 at 5232.208 kb on - strand, within AO356_23330 at 5232.208 kb on - strand, within AO356_23330 at 5232.348 kb on + strand, within AO356_23330 at 5232.348 kb on + strand, within AO356_23330 at 5232.348 kb on + strand, within AO356_23330 at 5232.487 kb on + strand at 5232.488 kb on - strand at 5232.513 kb on + strand at 5232.545 kb on - strand at 5232.583 kb on + strand at 5232.583 kb on + strand at 5232.584 kb on - strand at 5232.584 kb on - strand at 5232.585 kb on + strand at 5232.585 kb on + strand at 5232.585 kb on + strand at 5232.586 kb on - strand at 5232.586 kb on - strand at 5232.586 kb on - strand at 5232.586 kb on - strand at 5232.586 kb on - strand at 5232.655 kb on + strand at 5232.655 kb on + strand at 5232.655 kb on + strand at 5232.713 kb on - strand at 5233.612 kb on - strand, within AO356_23340 at 5233.783 kb on + strand, within AO356_23340 at 5233.784 kb on - strand, within AO356_23340 at 5233.784 kb on - strand, within AO356_23340 at 5233.836 kb on + strand, within AO356_23340 at 5234.018 kb on - strand, within AO356_23340 at 5234.042 kb on - strand, within AO356_23340
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 5,231,991 + AO356_23330 0.53 +0.2 5,231,992 - AO356_23330 0.53 +0.1 5,231,992 - AO356_23330 0.53 +0.2 5,232,033 + AO356_23330 0.57 +0.8 5,232,033 + AO356_23330 0.57 -0.7 5,232,081 - AO356_23330 0.61 +0.5 5,232,165 + AO356_23330 0.69 -2.3 5,232,166 - AO356_23330 0.69 +0.3 5,232,166 - AO356_23330 0.69 +0.2 5,232,207 + AO356_23330 0.73 +1.1 5,232,207 + AO356_23330 0.73 -1.4 5,232,207 + AO356_23330 0.73 +0.4 5,232,207 + AO356_23330 0.73 +0.0 5,232,207 + AO356_23330 0.73 +0.6 5,232,208 - AO356_23330 0.73 -0.5 5,232,208 - AO356_23330 0.73 -0.3 5,232,208 - AO356_23330 0.73 -0.3 5,232,348 + AO356_23330 0.86 +0.2 5,232,348 + AO356_23330 0.86 +0.6 5,232,348 + AO356_23330 0.86 -1.2 5,232,487 + -0.6 5,232,488 - +0.1 5,232,513 + +0.2 5,232,545 - +0.5 5,232,583 + +1.0 5,232,583 + +0.4 5,232,584 - -2.2 5,232,584 - -0.5 5,232,585 + +0.3 5,232,585 + +0.2 5,232,585 + +0.4 5,232,586 - +0.0 5,232,586 - -0.3 5,232,586 - -3.0 5,232,586 - +0.8 5,232,586 - +0.1 5,232,655 + +0.2 5,232,655 + +0.6 5,232,655 + -2.2 5,232,713 - +0.0 5,233,612 - AO356_23340 0.17 +1.8 5,233,783 + AO356_23340 0.26 +0.4 5,233,784 - AO356_23340 0.26 -0.2 5,233,784 - AO356_23340 0.26 +0.1 5,233,836 + AO356_23340 0.28 +0.1 5,234,018 - AO356_23340 0.37 -0.3 5,234,042 - AO356_23340 0.39 -0.5
Or see this region's nucleotide sequence