Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_22125

Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
4,951,630	+		AO356_22120	0.47	-1.0
4,951,630	+		AO356_22120	0.47	-0.0
4,951,721	+		AO356_22120	0.57	-0.8
4,951,721	+		AO356_22120	0.57	-0.2
4,951,722	-		AO356_22120	0.57	-0.2
4,951,722	-		AO356_22120	0.57	+0.5
4,951,753	-		AO356_22120	0.61	-0.5
4,951,798	+		AO356_22120	0.66	+0.5
4,951,799	-		AO356_22120	0.66	+0.4
4,951,863	+		AO356_22120	0.73	-0.0
4,951,864	-		AO356_22120	0.74	-0.8
4,951,864	-		AO356_22120	0.74	-0.3
4,951,904	-		AO356_22120	0.78	+0.7
4,951,914	-		AO356_22120	0.79	-1.3
4,951,930	+		AO356_22120	0.81	-1.2
4,951,956	-		AO356_22120	0.84	+0.5
4,951,966	+		AO356_22120	0.85	-0.0
4,951,966	+		AO356_22120	0.85	-1.6
4,951,967	-		AO356_22120	0.85	-0.0
4,951,969	+		AO356_22120	0.85	+0.6
4,952,003	-		AO356_22120	0.89	+0.5
4,952,042	+				+0.2
4,952,043	-				+1.2
4,952,043	-				-1.0
4,952,043	-				+0.4
4,952,138	+				+0.8
4,952,153	+				-0.6
4,952,231	+				+0.1
4,952,231	+				-0.2
4,952,239	+				+0.2
4,952,240	-				+0.4
4,952,240	-				+0.4
4,952,357	+				+0.4
4,952,357	+				-0.7
4,952,358	-				+0.5
4,952,391	+				+0.6
4,952,391	+				-0.6
4,952,391	+				+0.5
4,952,391	+				-1.4
4,952,391	+				-0.5
4,952,391	+				+0.5
4,952,391	+				+0.7
4,952,392	-				-0.5
4,952,392	-				-0.0
4,952,392	-				-0.7
4,952,392	-				-0.2
4,952,411	+				-1.0
4,952,411	+				+2.5
4,952,411	+				+0.2
4,952,412	-				-0.8
4,952,426	+				-0.3
4,952,426	+				+0.4
4,952,426	+				-0.5
4,952,509	+				+0.7
4,952,509	+				-0.5
4,952,509	+				-1.3
4,952,509	+				-0.6
4,952,509	+				+1.3
4,952,509	+				-1.0
4,952,509	+				-1.6
4,952,509	+				+0.8
4,952,509	+				+0.4
4,952,510	-				+1.0
4,952,510	-				+0.7
4,952,510	-				-1.0
4,952,510	-				+1.0
4,952,510	-				-0.0
4,952,536	+				-0.5
4,952,537	-				+0.5
4,952,562	-				-0.0
4,952,562	-				-0.5
4,952,562	-				-0.0
4,952,569	+				+0.4
4,952,569	+				+1.5
4,952,569	+				-0.6
4,952,569	+				-0.8
4,952,570	-				-0.0
4,952,570	-				+1.8
4,952,570	-				-0.0
4,952,647	+				+2.0
4,952,647	+				-0.0
4,952,647	+				+1.5
4,952,648	-				+1.3
4,952,648	-				+2.0
4,952,648	-				+0.5
4,952,651	-				+1.0
4,952,809	+		AO356_22125	0.11	+1.5
4,952,809	+		AO356_22125	0.11	-1.0
4,952,810	-		AO356_22125	0.11	+0.7
4,952,933	-		AO356_22125	0.16	-0.0
4,952,933	-		AO356_22125	0.16	-0.0
4,953,149	-		AO356_22125	0.26	-0.0
4,953,149	-		AO356_22125	0.26	+1.5
4,953,281	-		AO356_22125	0.32	+1.0
4,953,281	-		AO356_22125	0.32	-0.0
4,953,536	-		AO356_22125	0.43	-0.6
4,953,638	-		AO356_22125	0.48	-0.0
4,953,649	+		AO356_22125	0.49	+0.4
4,953,827	-		AO356_22125	0.57	+0.8
4,953,968	+		AO356_22125	0.63	+1.5
4,953,968	+		AO356_22125	0.63	+0.5
4,953,969	-		AO356_22125	0.63	-1.0
4,953,971	-		AO356_22125	0.63	+1.0
4,953,971	-		AO356_22125	0.63	-0.0
4,954,146	+		AO356_22125	0.71	+0.6
4,954,146	+		AO356_22125	0.71	+2.0
4,954,147	-		AO356_22125	0.71	+0.4
4,954,147	-		AO356_22125	0.71	-0.0
4,954,275	+		AO356_22125	0.77	+1.2
4,954,276	-		AO356_22125	0.77	-0.0
4,954,333	+		AO356_22125	0.79	+1.3
4,954,333	+		AO356_22125	0.79	+1.2
4,954,334	-		AO356_22125	0.79	-0.4
4,954,334	-		AO356_22125	0.79	-1.4
4,954,732	+				-0.6
4,954,732	+				-1.0
4,954,732	+				-0.0
4,954,732	+				+1.4
4,954,733	-				-0.5
4,954,767	-				+1.0
4,954,767	-				+1.0
4,954,768	+				-0.3
4,954,768	+				-1.0
4,954,768	+				+0.4
4,954,768	+				+1.5
4,954,793	+				+1.8
4,954,793	+				-0.0
4,954,793	+				-0.4
4,954,793	+				-1.6
4,954,793	+				-0.0
4,954,818	+				-0.0
4,954,819	-				+0.4
4,954,852	-				-0.8
4,954,964	+				+0.5
4,954,964	+				+1.0
4,954,965	-				+1.0
4,954,965	-				-0.2
4,954,987	+				+1.7
4,954,987	+				-0.5
4,954,987	+				+0.7
4,955,029	+				+0.1
4,955,030	-				+0.6
4,955,030	-				+0.0
4,955,031	+				+0.4
4,955,031	+				-0.4
4,955,031	+				-0.5
4,955,031	+				-2.0
4,955,031	+				-0.4
4,955,031	+				-0.7
4,955,031	+				-0.5
4,955,031	+				-0.6
4,955,031	+				+2.3
4,955,031	+				-0.6
4,955,031	+				-1.5
4,955,032	-				-0.6
4,955,032	-				+1.0
4,955,032	-				-0.3
4,955,032	-				+0.6
4,955,032	-				+0.3
4,955,032	-				+0.5
4,955,032	-				+0.5
4,955,035	+				+1.0
4,955,035	+				+0.4
4,955,035	+				+2.2
4,955,035	+				-1.0
4,955,035	+				-0.2
4,955,035	+				+0.4
4,955,035	+				-1.2
4,955,035	+				+1.0
4,955,035	+				-1.2
4,955,035	+				-0.6
4,955,035	+				-0.0
4,955,035	+				-1.4
4,955,035	+				-1.8
4,955,035	+				-0.0
4,955,036	-				+0.3
4,955,036	-				-1.0
4,955,036	-				+0.4
4,955,036	-				-0.4
4,955,036	-				+0.5
4,955,036	-				+1.0
4,955,036	-				+1.5
4,955,039	+				-0.0
4,955,039	+				-0.0
4,955,039	+				+0.1
4,955,040	-				+1.1
4,955,040	-				+0.5
4,955,040	-				+1.0
4,955,040	-				+1.5
4,955,040	-				+0.7
4,955,040	-				-0.1
4,955,040	-				+0.1
4,955,065	-				+1.0
4,955,118	+		AO356_22130	0.15	-1.8
4,955,118	+		AO356_22130	0.15	-1.3
4,955,197	-		AO356_22130	0.57	-0.7
4,955,201	+		AO356_22130	0.59	-0.9
4,955,202	-		AO356_22130	0.60	-0.7
4,955,202	-		AO356_22130	0.60	-2.0
4,955,202	-		AO356_22130	0.60	-0.6
4,955,282	+				-0.0
4,955,344	+				+0.4
4,955,344	+				+1.4
4,955,345	-				+0.3
4,955,345	-				-0.2
4,955,345	-				-0.0
4,955,345	-				-0.2
4,955,345	-				-0.2
4,955,451	-		AO356_22135	0.14	-0.8
4,955,487	-		AO356_22135	0.17	+0.4
4,955,487	-		AO356_22135	0.17	-0.4
4,955,487	-		AO356_22135	0.17	+0.4
4,955,513	+		AO356_22135	0.19	-0.3
4,955,513	+		AO356_22135	0.19	+0.5
4,955,564	+		AO356_22135	0.23	-0.6
4,955,564	+		AO356_22135	0.23	+0.1
4,955,564	+		AO356_22135	0.23	-0.6
4,955,564	+		AO356_22135	0.23	-1.6
4,955,565	-		AO356_22135	0.23	-0.0
4,955,565	-		AO356_22135	0.23	-0.2
4,955,628	+		AO356_22135	0.28	-0.7
4,955,629	-		AO356_22135	0.28	-0.5
4,955,629	-		AO356_22135	0.28	+0.7
4,955,654	+		AO356_22135	0.30	+0.1
4,955,654	+		AO356_22135	0.30	-0.3
4,955,654	+		AO356_22135	0.30	-2.4
4,955,654	+		AO356_22135	0.30	-0.3
4,955,655	-		AO356_22135	0.30	-0.4
4,955,655	-		AO356_22135	0.30	+2.0
4,955,655	-		AO356_22135	0.30	-1.0
4,955,655	-		AO356_22135	0.30	+0.6
4,955,655	-		AO356_22135	0.30	-0.0
4,955,655	-		AO356_22135	0.30	+0.0
4,955,760	-		AO356_22135	0.39	-1.3
4,955,760	-		AO356_22135	0.39	+0.7
4,955,760	-		AO356_22135	0.39	+0.1
4,955,760	-		AO356_22135	0.39	-0.5