Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21340 and AO356_21345 are separated by 146 nucleotides AO356_21345 and AO356_21350 are separated by 79 nucleotides AO356_21350 and AO356_21355 are separated by 53 nucleotides AO356_21355 and AO356_21360 are separated by 75 nucleotides AO356_21360 and AO356_21365 are separated by 181 nucleotides AO356_21365 and AO356_21370 are separated by 28 nucleotides
AO356_21340: AO356_21340 - glutamate--tRNA ligase, at 4,787,295 to 4,788,776
_21340
AO356_21345: AO356_21345 - tRNA-Ala, at 4,788,923 to 4,788,998
_21345
AO356_21350: AO356_21350 - tRNA-Glu, at 4,789,078 to 4,789,153
_21350
AO356_21355: AO356_21355 - tRNA-Ala, at 4,789,207 to 4,789,282
_21355
AO356_21360: AO356_21360 - tRNA-Glu, at 4,789,358 to 4,789,433
_21360
AO356_21365: AO356_21365 - TetR family transcriptional regulator, at 4,789,615 to 4,790,154
_21365
AO356_21370: AO356_21370 - hydrolase, at 4,790,183 to 4,791,016
_21370
Position (kb)
4789
4790 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4788.774 kb on + strand at 4788.856 kb on - strand at 4788.988 kb on + strand, within AO356_21345 at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.063 kb on + strand at 4789.065 kb on + strand at 4789.065 kb on + strand at 4789.065 kb on + strand at 4789.065 kb on + strand at 4789.158 kb on + strand at 4789.272 kb on + strand, within AO356_21355 at 4789.285 kb on + strand at 4789.346 kb on + strand at 4789.346 kb on + strand at 4789.346 kb on + strand at 4789.457 kb on + strand at 4789.747 kb on + strand, within AO356_21365 at 4789.748 kb on - strand, within AO356_21365 at 4789.882 kb on + strand, within AO356_21365 at 4789.882 kb on + strand, within AO356_21365 at 4789.882 kb on + strand, within AO356_21365 at 4789.883 kb on - strand, within AO356_21365 at 4789.883 kb on - strand, within AO356_21365 at 4789.883 kb on - strand, within AO356_21365 at 4789.992 kb on + strand, within AO356_21365 at 4789.992 kb on + strand, within AO356_21365 at 4790.202 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.335 kb on + strand, within AO356_21370 at 4790.336 kb on - strand, within AO356_21370 at 4790.336 kb on - strand, within AO356_21370
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 4,788,774 + +0.1 4,788,856 - +1.0 4,788,988 + AO356_21345 0.86 -0.0 4,789,063 + +1.3 4,789,063 + +0.4 4,789,063 + -0.2 4,789,063 + +3.2 4,789,063 + -1.6 4,789,063 + -0.0 4,789,063 + -1.2 4,789,063 + -0.9 4,789,063 + -0.7 4,789,063 + +0.9 4,789,065 + +0.4 4,789,065 + +1.3 4,789,065 + +0.6 4,789,065 + -0.0 4,789,158 + -0.5 4,789,272 + AO356_21355 0.86 +0.5 4,789,285 + +2.0 4,789,346 + -1.2 4,789,346 + +0.6 4,789,346 + -0.2 4,789,457 + +0.1 4,789,747 + AO356_21365 0.24 +0.2 4,789,748 - AO356_21365 0.25 -0.9 4,789,882 + AO356_21365 0.49 +0.2 4,789,882 + AO356_21365 0.49 +0.4 4,789,882 + AO356_21365 0.49 -0.0 4,789,883 - AO356_21365 0.50 -1.0 4,789,883 - AO356_21365 0.50 -1.2 4,789,883 - AO356_21365 0.50 -0.3 4,789,992 + AO356_21365 0.70 -1.8 4,789,992 + AO356_21365 0.70 -2.2 4,790,202 + +0.2 4,790,206 + +1.0 4,790,206 + -0.1 4,790,206 + -0.4 4,790,206 + -0.2 4,790,206 + -0.1 4,790,206 + -0.2 4,790,206 + +0.2 4,790,206 + +1.2 4,790,206 + -2.6 4,790,206 + -0.7 4,790,207 - +0.7 4,790,207 - -0.0 4,790,207 - -0.2 4,790,207 - -0.0 4,790,207 - -0.0 4,790,335 + AO356_21370 0.18 +0.6 4,790,336 - AO356_21370 0.18 -0.9 4,790,336 - AO356_21370 0.18 -0.2
Or see this region's nucleotide sequence