Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_19525 and AO356_19530 are separated by 139 nucleotides AO356_19530 and AO356_19535 are separated by 106 nucleotides
AO356_19525: AO356_19525 - protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific, at 4,379,913 to 4,380,821
_19525
AO356_19530: AO356_19530 - hypothetical protein, at 4,380,961 to 4,381,749
_19530
AO356_19535: AO356_19535 - alpha/beta hydrolase, at 4,381,856 to 4,382,821
_19535
Position (kb)
4380
4381
4382 Strain fitness (log2 ratio)
-1
0
1
2 at 4380.074 kb on + strand, within AO356_19525 at 4380.074 kb on + strand, within AO356_19525 at 4380.074 kb on + strand, within AO356_19525 at 4380.078 kb on + strand, within AO356_19525 at 4380.078 kb on + strand, within AO356_19525 at 4380.078 kb on + strand, within AO356_19525 at 4380.079 kb on - strand, within AO356_19525 at 4380.165 kb on + strand, within AO356_19525 at 4380.165 kb on + strand, within AO356_19525 at 4380.165 kb on + strand, within AO356_19525 at 4380.830 kb on - strand at 4380.830 kb on - strand at 4380.842 kb on - strand at 4380.942 kb on + strand at 4380.942 kb on + strand at 4380.942 kb on + strand at 4380.943 kb on - strand at 4380.953 kb on + strand at 4381.352 kb on + strand, within AO356_19530 at 4381.380 kb on + strand, within AO356_19530 at 4381.577 kb on + strand, within AO356_19530 at 4381.577 kb on + strand, within AO356_19530 at 4381.577 kb on + strand, within AO356_19530 at 4381.577 kb on + strand, within AO356_19530 at 4381.577 kb on + strand, within AO356_19530 at 4381.577 kb on + strand, within AO356_19530 at 4381.577 kb on + strand, within AO356_19530 at 4381.578 kb on - strand, within AO356_19530 at 4381.578 kb on - strand, within AO356_19530 at 4381.578 kb on - strand, within AO356_19530 at 4381.578 kb on - strand, within AO356_19530 at 4381.578 kb on - strand, within AO356_19530 at 4381.578 kb on - strand, within AO356_19530 at 4381.578 kb on - strand, within AO356_19530 at 4381.749 kb on + strand at 4381.829 kb on + strand at 4381.838 kb on + strand at 4381.838 kb on + strand at 4381.839 kb on - strand at 4381.839 kb on - strand at 4381.880 kb on - strand at 4381.935 kb on + strand at 4381.936 kb on - strand at 4382.151 kb on + strand, within AO356_19535 at 4382.151 kb on + strand, within AO356_19535 at 4382.151 kb on + strand, within AO356_19535 at 4382.152 kb on - strand, within AO356_19535 at 4382.152 kb on - strand, within AO356_19535 at 4382.159 kb on + strand, within AO356_19535 at 4382.159 kb on + strand, within AO356_19535 at 4382.160 kb on - strand, within AO356_19535 at 4382.160 kb on - strand, within AO356_19535 at 4382.160 kb on - strand, within AO356_19535 at 4382.160 kb on - strand, within AO356_19535 at 4382.160 kb on - strand, within AO356_19535 at 4382.294 kb on + strand, within AO356_19535 at 4382.294 kb on + strand, within AO356_19535 at 4382.295 kb on - strand, within AO356_19535 at 4382.466 kb on + strand, within AO356_19535 at 4382.466 kb on + strand, within AO356_19535 at 4382.466 kb on + strand, within AO356_19535 at 4382.466 kb on + strand, within AO356_19535 at 4382.466 kb on + strand, within AO356_19535 at 4382.466 kb on + strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.467 kb on - strand, within AO356_19535 at 4382.632 kb on + strand, within AO356_19535
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 4,380,074 + AO356_19525 0.18 -0.2 4,380,074 + AO356_19525 0.18 +0.4 4,380,074 + AO356_19525 0.18 -1.1 4,380,078 + AO356_19525 0.18 +1.8 4,380,078 + AO356_19525 0.18 +0.1 4,380,078 + AO356_19525 0.18 -0.5 4,380,079 - AO356_19525 0.18 -1.1 4,380,165 + AO356_19525 0.28 +1.5 4,380,165 + AO356_19525 0.28 -0.9 4,380,165 + AO356_19525 0.28 -0.9 4,380,830 - +0.1 4,380,830 - -0.3 4,380,842 - -0.2 4,380,942 + +0.3 4,380,942 + +0.7 4,380,942 + +1.3 4,380,943 - +0.1 4,380,953 + -0.3 4,381,352 + AO356_19530 0.50 -0.5 4,381,380 + AO356_19530 0.53 +0.6 4,381,577 + AO356_19530 0.78 +1.7 4,381,577 + AO356_19530 0.78 -0.0 4,381,577 + AO356_19530 0.78 -1.3 4,381,577 + AO356_19530 0.78 -0.1 4,381,577 + AO356_19530 0.78 +0.5 4,381,577 + AO356_19530 0.78 +1.2 4,381,577 + AO356_19530 0.78 +0.6 4,381,578 - AO356_19530 0.78 +0.2 4,381,578 - AO356_19530 0.78 +0.5 4,381,578 - AO356_19530 0.78 +0.7 4,381,578 - AO356_19530 0.78 -1.2 4,381,578 - AO356_19530 0.78 +0.1 4,381,578 - AO356_19530 0.78 -0.1 4,381,578 - AO356_19530 0.78 +0.1 4,381,749 + +0.5 4,381,829 + +0.7 4,381,838 + -0.8 4,381,838 + +0.8 4,381,839 - -0.0 4,381,839 - -0.3 4,381,880 - -0.3 4,381,935 + -0.2 4,381,936 - +0.2 4,382,151 + AO356_19535 0.31 +0.8 4,382,151 + AO356_19535 0.31 -0.0 4,382,151 + AO356_19535 0.31 +0.6 4,382,152 - AO356_19535 0.31 -0.5 4,382,152 - AO356_19535 0.31 -0.0 4,382,159 + AO356_19535 0.31 +0.5 4,382,159 + AO356_19535 0.31 +0.5 4,382,160 - AO356_19535 0.31 -0.5 4,382,160 - AO356_19535 0.31 -0.2 4,382,160 - AO356_19535 0.31 +0.6 4,382,160 - AO356_19535 0.31 +0.2 4,382,160 - AO356_19535 0.31 +0.4 4,382,294 + AO356_19535 0.45 -0.3 4,382,294 + AO356_19535 0.45 -1.1 4,382,295 - AO356_19535 0.45 -0.2 4,382,466 + AO356_19535 0.63 +0.4 4,382,466 + AO356_19535 0.63 -1.3 4,382,466 + AO356_19535 0.63 -0.1 4,382,466 + AO356_19535 0.63 -0.2 4,382,466 + AO356_19535 0.63 +0.4 4,382,466 + AO356_19535 0.63 +1.9 4,382,467 - AO356_19535 0.63 -0.3 4,382,467 - AO356_19535 0.63 +0.3 4,382,467 - AO356_19535 0.63 +0.1 4,382,467 - AO356_19535 0.63 -0.2 4,382,467 - AO356_19535 0.63 -0.7 4,382,467 - AO356_19535 0.63 +0.1 4,382,467 - AO356_19535 0.63 +0.1 4,382,467 - AO356_19535 0.63 +0.1 4,382,467 - AO356_19535 0.63 -1.2 4,382,467 - AO356_19535 0.63 -0.0 4,382,632 + AO356_19535 0.80 -1.1
Or see this region's nucleotide sequence