Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_09210 and AO356_09215 overlap by 4 nucleotides AO356_09215 and AO356_09220 overlap by 4 nucleotides AO356_09220 and AO356_09225 overlap by 1 nucleotides
AO356_09210: AO356_09210 - 3-oxoacyl-ACP synthase, at 2,081,927 to 2,083,123
_09210
AO356_09215: AO356_09215 - 3-hydroxylacyl-ACP dehydratase, at 2,083,120 to 2,083,587
_09215
AO356_09220: AO356_09220 - 3-ketoacyl-ACP reductase, at 2,083,584 to 2,084,312
_09220
AO356_09225: AO356_09225 - 3-oxoacyl-ACP synthase, at 2,084,312 to 2,085,538
_09225
Position (kb)
2083
2084
2085 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2082.677 kb on - strand, within AO356_09210 at 2082.677 kb on - strand, within AO356_09210 at 2082.767 kb on + strand, within AO356_09210 at 2082.767 kb on + strand, within AO356_09210 at 2082.768 kb on - strand, within AO356_09210 at 2083.345 kb on - strand, within AO356_09215 at 2083.550 kb on - strand at 2083.550 kb on - strand at 2083.550 kb on - strand at 2083.550 kb on - strand at 2083.827 kb on + strand, within AO356_09220 at 2083.827 kb on + strand, within AO356_09220 at 2083.929 kb on + strand, within AO356_09220 at 2083.929 kb on + strand, within AO356_09220 at 2083.930 kb on - strand, within AO356_09220 at 2083.930 kb on - strand, within AO356_09220 at 2083.936 kb on + strand, within AO356_09220 at 2083.936 kb on + strand, within AO356_09220 at 2083.936 kb on + strand, within AO356_09220 at 2083.936 kb on + strand, within AO356_09220 at 2083.936 kb on + strand, within AO356_09220 at 2083.936 kb on + strand, within AO356_09220 at 2083.937 kb on - strand, within AO356_09220 at 2083.937 kb on - strand, within AO356_09220 at 2083.937 kb on - strand, within AO356_09220 at 2083.937 kb on - strand, within AO356_09220 at 2084.040 kb on + strand, within AO356_09220 at 2084.040 kb on + strand, within AO356_09220 at 2084.040 kb on + strand, within AO356_09220 at 2084.040 kb on + strand, within AO356_09220 at 2084.040 kb on + strand, within AO356_09220 at 2084.041 kb on - strand, within AO356_09220 at 2084.041 kb on - strand, within AO356_09220 at 2084.041 kb on - strand, within AO356_09220 at 2084.041 kb on - strand, within AO356_09220 at 2084.041 kb on - strand, within AO356_09220 at 2084.041 kb on - strand, within AO356_09220 at 2084.168 kb on - strand, within AO356_09220 at 2084.269 kb on - strand at 2084.714 kb on + strand, within AO356_09225 at 2084.715 kb on - strand, within AO356_09225 at 2084.715 kb on - strand, within AO356_09225 at 2084.715 kb on - strand, within AO356_09225 at 2084.715 kb on - strand, within AO356_09225 at 2084.834 kb on + strand, within AO356_09225 at 2084.834 kb on + strand, within AO356_09225 at 2084.834 kb on + strand, within AO356_09225 at 2084.835 kb on - strand, within AO356_09225 at 2084.927 kb on + strand, within AO356_09225 at 2085.201 kb on - strand, within AO356_09225
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 2,082,677 - AO356_09210 0.63 +0.7 2,082,677 - AO356_09210 0.63 +1.4 2,082,767 + AO356_09210 0.70 -0.3 2,082,767 + AO356_09210 0.70 -1.2 2,082,768 - AO356_09210 0.70 -1.1 2,083,345 - AO356_09215 0.48 -0.2 2,083,550 - +0.6 2,083,550 - -1.5 2,083,550 - -1.6 2,083,550 - +1.0 2,083,827 + AO356_09220 0.33 -1.2 2,083,827 + AO356_09220 0.33 -0.8 2,083,929 + AO356_09220 0.47 +0.4 2,083,929 + AO356_09220 0.47 -0.2 2,083,930 - AO356_09220 0.47 -0.3 2,083,930 - AO356_09220 0.47 -0.5 2,083,936 + AO356_09220 0.48 +0.3 2,083,936 + AO356_09220 0.48 -0.2 2,083,936 + AO356_09220 0.48 -0.6 2,083,936 + AO356_09220 0.48 -1.9 2,083,936 + AO356_09220 0.48 +0.8 2,083,936 + AO356_09220 0.48 -0.1 2,083,937 - AO356_09220 0.48 +0.2 2,083,937 - AO356_09220 0.48 +0.1 2,083,937 - AO356_09220 0.48 -0.9 2,083,937 - AO356_09220 0.48 -0.8 2,084,040 + AO356_09220 0.63 +0.7 2,084,040 + AO356_09220 0.63 -1.2 2,084,040 + AO356_09220 0.63 +2.0 2,084,040 + AO356_09220 0.63 -1.9 2,084,040 + AO356_09220 0.63 +1.4 2,084,041 - AO356_09220 0.63 -0.6 2,084,041 - AO356_09220 0.63 -0.6 2,084,041 - AO356_09220 0.63 +0.7 2,084,041 - AO356_09220 0.63 -0.1 2,084,041 - AO356_09220 0.63 +0.4 2,084,041 - AO356_09220 0.63 -2.4 2,084,168 - AO356_09220 0.80 +2.4 2,084,269 - +0.2 2,084,714 + AO356_09225 0.33 -0.1 2,084,715 - AO356_09225 0.33 -0.9 2,084,715 - AO356_09225 0.33 +1.4 2,084,715 - AO356_09225 0.33 -0.2 2,084,715 - AO356_09225 0.33 -1.0 2,084,834 + AO356_09225 0.43 +0.4 2,084,834 + AO356_09225 0.43 -0.7 2,084,834 + AO356_09225 0.43 -0.3 2,084,835 - AO356_09225 0.43 +0.4 2,084,927 + AO356_09225 0.50 +1.0 2,085,201 - AO356_09225 0.72 -1.2
Or see this region's nucleotide sequence