Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_01925 and AO356_01930 are separated by 15 nucleotides AO356_01930 and AO356_01935 overlap by 10 nucleotides AO356_01935 and AO356_01940 are separated by 254 nucleotides
AO356_01925: AO356_01925 - hypothetical protein, at 427,543 to 427,905
_01925
AO356_01930: AO356_01930 - hypothetical protein, at 427,921 to 428,799
_01930
AO356_01935: AO356_01935 - biotin synthase, at 428,790 to 430,010
_01935
AO356_01940: AO356_01940 - hypothetical protein, at 430,265 to 430,588
_01940
Position (kb) 428
429
430
431 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 427.795 kb on - strand, within AO356_01925 at 427.901 kb on + strand at 427.902 kb on - strand at 427.922 kb on + strand at 427.922 kb on + strand at 427.922 kb on + strand at 427.922 kb on + strand at 427.946 kb on - strand at 427.975 kb on - strand at 428.156 kb on + strand, within AO356_01930 at 428.156 kb on + strand, within AO356_01930 at 428.156 kb on + strand, within AO356_01930 at 428.156 kb on + strand, within AO356_01930 at 428.156 kb on + strand, within AO356_01930 at 428.156 kb on + strand, within AO356_01930 at 428.156 kb on + strand, within AO356_01930 at 428.156 kb on + strand, within AO356_01930 at 428.159 kb on + strand, within AO356_01930 at 428.160 kb on - strand, within AO356_01930 at 428.160 kb on - strand, within AO356_01930 at 428.160 kb on - strand, within AO356_01930 at 428.160 kb on - strand, within AO356_01930 at 428.160 kb on - strand, within AO356_01930 at 428.549 kb on + strand, within AO356_01930 at 428.549 kb on + strand, within AO356_01930 at 428.549 kb on + strand, within AO356_01930 at 428.550 kb on - strand, within AO356_01930 at 428.550 kb on - strand, within AO356_01930 at 428.550 kb on - strand, within AO356_01930 at 428.947 kb on + strand, within AO356_01935 at 428.993 kb on + strand, within AO356_01935 at 428.994 kb on - strand, within AO356_01935 at 429.014 kb on + strand, within AO356_01935 at 429.149 kb on - strand, within AO356_01935 at 429.182 kb on - strand, within AO356_01935 at 429.262 kb on + strand, within AO356_01935 at 429.262 kb on + strand, within AO356_01935 at 429.265 kb on + strand, within AO356_01935 at 429.265 kb on + strand, within AO356_01935 at 429.265 kb on + strand, within AO356_01935 at 429.266 kb on - strand, within AO356_01935 at 429.266 kb on - strand, within AO356_01935 at 429.322 kb on + strand, within AO356_01935 at 429.556 kb on - strand, within AO356_01935 at 429.590 kb on + strand, within AO356_01935 at 429.592 kb on + strand, within AO356_01935 at 429.592 kb on + strand, within AO356_01935 at 429.593 kb on - strand, within AO356_01935 at 429.593 kb on - strand, within AO356_01935 at 429.593 kb on - strand, within AO356_01935 at 429.593 kb on - strand, within AO356_01935 at 429.593 kb on - strand, within AO356_01935 at 429.593 kb on - strand, within AO356_01935 at 429.593 kb on - strand, within AO356_01935 at 429.637 kb on - strand, within AO356_01935 at 429.656 kb on - strand, within AO356_01935 at 429.656 kb on - strand, within AO356_01935 at 429.796 kb on + strand, within AO356_01935 at 429.796 kb on + strand, within AO356_01935 at 429.796 kb on + strand, within AO356_01935 at 429.809 kb on - strand, within AO356_01935 at 429.809 kb on - strand, within AO356_01935 at 429.866 kb on + strand, within AO356_01935 at 429.867 kb on - strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.868 kb on + strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.869 kb on - strand, within AO356_01935 at 429.872 kb on + strand, within AO356_01935 at 429.872 kb on + strand, within AO356_01935 at 429.872 kb on + strand, within AO356_01935 at 429.872 kb on + strand, within AO356_01935 at 429.873 kb on - strand, within AO356_01935 at 429.881 kb on + strand, within AO356_01935 at 429.881 kb on + strand, within AO356_01935 at 429.881 kb on + strand, within AO356_01935 at 429.882 kb on - strand, within AO356_01935 at 429.882 kb on - strand, within AO356_01935 at 429.926 kb on + strand at 429.926 kb on + strand at 429.926 kb on + strand at 429.926 kb on + strand at 429.927 kb on - strand at 429.927 kb on - strand at 429.964 kb on + strand at 429.965 kb on - strand at 430.001 kb on + strand at 430.034 kb on + strand at 430.117 kb on + strand at 430.117 kb on + strand at 430.117 kb on + strand at 430.140 kb on + strand at 430.140 kb on + strand at 430.140 kb on + strand at 430.141 kb on - strand at 430.142 kb on + strand at 430.210 kb on + strand at 430.211 kb on - strand at 430.211 kb on - strand at 430.237 kb on + strand at 430.238 kb on - strand at 430.534 kb on - strand, within AO356_01940 at 430.534 kb on - strand, within AO356_01940 at 430.626 kb on - strand at 430.636 kb on + strand at 430.637 kb on - strand at 430.637 kb on - strand at 430.654 kb on + strand at 430.654 kb on + strand at 430.655 kb on - strand at 430.754 kb on + strand at 430.840 kb on - strand at 430.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 427,795 - AO356_01925 0.69 -0.7 427,901 + +0.4 427,902 - +0.9 427,922 + +0.9 427,922 + +0.8 427,922 + +0.3 427,922 + -0.3 427,946 - -0.1 427,975 - -0.2 428,156 + AO356_01930 0.27 +0.6 428,156 + AO356_01930 0.27 +0.2 428,156 + AO356_01930 0.27 +0.9 428,156 + AO356_01930 0.27 -0.1 428,156 + AO356_01930 0.27 +0.5 428,156 + AO356_01930 0.27 +0.5 428,156 + AO356_01930 0.27 +0.5 428,156 + AO356_01930 0.27 -0.6 428,159 + AO356_01930 0.27 +0.9 428,160 - AO356_01930 0.27 -0.2 428,160 - AO356_01930 0.27 +0.1 428,160 - AO356_01930 0.27 -0.5 428,160 - AO356_01930 0.27 +0.4 428,160 - AO356_01930 0.27 +1.7 428,549 + AO356_01930 0.71 +0.1 428,549 + AO356_01930 0.71 +0.5 428,549 + AO356_01930 0.71 +0.1 428,550 - AO356_01930 0.72 -0.4 428,550 - AO356_01930 0.72 +1.4 428,550 - AO356_01930 0.72 +0.1 428,947 + AO356_01935 0.13 +0.1 428,993 + AO356_01935 0.17 +0.5 428,994 - AO356_01935 0.17 +0.7 429,014 + AO356_01935 0.18 -1.4 429,149 - AO356_01935 0.29 +0.3 429,182 - AO356_01935 0.32 -0.4 429,262 + AO356_01935 0.39 -0.1 429,262 + AO356_01935 0.39 +1.5 429,265 + AO356_01935 0.39 -0.3 429,265 + AO356_01935 0.39 -0.2 429,265 + AO356_01935 0.39 +0.4 429,266 - AO356_01935 0.39 +0.4 429,266 - AO356_01935 0.39 +1.1 429,322 + AO356_01935 0.44 -0.1 429,556 - AO356_01935 0.63 -0.6 429,590 + AO356_01935 0.66 -2.1 429,592 + AO356_01935 0.66 +0.6 429,592 + AO356_01935 0.66 +2.8 429,593 - AO356_01935 0.66 -0.8 429,593 - AO356_01935 0.66 -1.4 429,593 - AO356_01935 0.66 -0.4 429,593 - AO356_01935 0.66 -0.5 429,593 - AO356_01935 0.66 -0.5 429,593 - AO356_01935 0.66 -0.6 429,593 - AO356_01935 0.66 -0.9 429,637 - AO356_01935 0.69 -0.7 429,656 - AO356_01935 0.71 -0.1 429,656 - AO356_01935 0.71 +0.3 429,796 + AO356_01935 0.82 -0.5 429,796 + AO356_01935 0.82 +0.1 429,796 + AO356_01935 0.82 -1.6 429,809 - AO356_01935 0.83 +0.2 429,809 - AO356_01935 0.83 -0.6 429,866 + AO356_01935 0.88 -0.5 429,867 - AO356_01935 0.88 -0.7 429,868 + AO356_01935 0.88 +0.1 429,868 + AO356_01935 0.88 +0.4 429,868 + AO356_01935 0.88 -0.2 429,868 + AO356_01935 0.88 +0.4 429,868 + AO356_01935 0.88 +0.9 429,868 + AO356_01935 0.88 -0.3 429,868 + AO356_01935 0.88 +0.6 429,868 + AO356_01935 0.88 -1.6 429,868 + AO356_01935 0.88 +0.9 429,868 + AO356_01935 0.88 +0.4 429,868 + AO356_01935 0.88 -0.9 429,868 + AO356_01935 0.88 -1.6 429,868 + AO356_01935 0.88 -0.5 429,868 + AO356_01935 0.88 -0.6 429,868 + AO356_01935 0.88 +0.1 429,868 + AO356_01935 0.88 -0.4 429,869 - AO356_01935 0.88 +0.6 429,869 - AO356_01935 0.88 +0.5 429,869 - AO356_01935 0.88 +1.1 429,869 - AO356_01935 0.88 +0.4 429,869 - AO356_01935 0.88 +1.1 429,869 - AO356_01935 0.88 -0.1 429,869 - AO356_01935 0.88 -0.4 429,869 - AO356_01935 0.88 +0.1 429,869 - AO356_01935 0.88 -0.2 429,869 - AO356_01935 0.88 +0.9 429,869 - AO356_01935 0.88 +1.1 429,869 - AO356_01935 0.88 -0.1 429,869 - AO356_01935 0.88 +0.5 429,869 - AO356_01935 0.88 +0.5 429,869 - AO356_01935 0.88 +0.1 429,869 - AO356_01935 0.88 +1.7 429,869 - AO356_01935 0.88 +0.5 429,869 - AO356_01935 0.88 -0.3 429,872 + AO356_01935 0.89 -0.3 429,872 + AO356_01935 0.89 -1.4 429,872 + AO356_01935 0.89 -0.4 429,872 + AO356_01935 0.89 -0.9 429,873 - AO356_01935 0.89 +0.5 429,881 + AO356_01935 0.89 +0.8 429,881 + AO356_01935 0.89 -1.4 429,881 + AO356_01935 0.89 -0.2 429,882 - AO356_01935 0.89 -0.3 429,882 - AO356_01935 0.89 +0.4 429,926 + +0.8 429,926 + -0.4 429,926 + -0.8 429,926 + -0.1 429,927 - +1.3 429,927 - +0.4 429,964 + +1.4 429,965 - -0.3 430,001 + +0.8 430,034 + -0.5 430,117 + -0.7 430,117 + -0.8 430,117 + -1.0 430,140 + -1.6 430,140 + -0.4 430,140 + -0.0 430,141 - +0.8 430,142 + -0.0 430,210 + +1.0 430,211 - -0.0 430,211 - -0.0 430,237 + +1.0 430,238 - +0.2 430,534 - AO356_01940 0.83 +0.9 430,534 - AO356_01940 0.83 +1.0 430,626 - -1.6 430,636 + -1.6 430,637 - -0.0 430,637 - +0.5 430,654 + +0.5 430,654 + +0.7 430,655 - +0.3 430,754 + +1.8 430,840 - +0.3 430,840 - +0.6
Or see this region's nucleotide sequence