Experiment: 48 hours of 100ug/mL streptomycin treatment after 3 hour phosphate starvation in M5G
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01560 and CCNA_01561 are separated by 154 nucleotides CCNA_01561 and CCNA_01562 overlap by 7 nucleotides CCNA_01562 and CCNA_01563 are separated by 6 nucleotides
CCNA_01560: CCNA_01560 - phosphoenolpyruvate carboxylase, at 1,676,914 to 1,679,643
_01560
CCNA_01561: CCNA_01561 - hypothetical protein, at 1,679,798 to 1,680,181
_01561
CCNA_01562: CCNA_01562 - 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase, at 1,680,175 to 1,680,849
_01562
CCNA_01563: CCNA_01563 - 2-dehydro-3-deoxygluconokinase, at 1,680,856 to 1,681,962
_01563
Position (kb)
1679
1680
1681 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1678.817 kb on - strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.901 kb on - strand, within CCNA_01560 at 1678.921 kb on + strand, within CCNA_01560 at 1678.921 kb on + strand, within CCNA_01560 at 1678.921 kb on + strand, within CCNA_01560 at 1678.974 kb on - strand, within CCNA_01560 at 1678.989 kb on + strand, within CCNA_01560 at 1678.989 kb on + strand, within CCNA_01560 at 1678.989 kb on + strand, within CCNA_01560 at 1678.993 kb on - strand, within CCNA_01560 at 1678.998 kb on - strand, within CCNA_01560 at 1678.998 kb on - strand, within CCNA_01560 at 1678.998 kb on - strand, within CCNA_01560 at 1679.001 kb on + strand, within CCNA_01560 at 1679.009 kb on + strand, within CCNA_01560 at 1679.017 kb on - strand, within CCNA_01560 at 1679.041 kb on + strand, within CCNA_01560 at 1679.127 kb on + strand, within CCNA_01560 at 1679.127 kb on + strand, within CCNA_01560 at 1679.142 kb on + strand, within CCNA_01560 at 1679.153 kb on + strand, within CCNA_01560 at 1679.153 kb on + strand, within CCNA_01560 at 1679.161 kb on + strand, within CCNA_01560 at 1679.161 kb on - strand, within CCNA_01560 at 1679.161 kb on - strand, within CCNA_01560 at 1679.202 kb on + strand, within CCNA_01560 at 1679.205 kb on - strand, within CCNA_01560 at 1679.288 kb on + strand, within CCNA_01560 at 1679.310 kb on + strand, within CCNA_01560 at 1679.314 kb on - strand, within CCNA_01560 at 1679.317 kb on + strand, within CCNA_01560 at 1679.370 kb on + strand, within CCNA_01560 at 1679.370 kb on + strand, within CCNA_01560 at 1679.378 kb on - strand at 1679.378 kb on - strand at 1679.378 kb on - strand at 1679.397 kb on + strand at 1679.409 kb on + strand at 1679.426 kb on + strand at 1679.426 kb on + strand at 1679.434 kb on - strand at 1679.460 kb on + strand at 1679.469 kb on - strand at 1679.494 kb on + strand at 1679.495 kb on - strand at 1679.513 kb on - strand at 1679.520 kb on + strand at 1679.858 kb on - strand, within CCNA_01561 at 1679.909 kb on - strand, within CCNA_01561 at 1679.955 kb on - strand, within CCNA_01561 at 1679.955 kb on - strand, within CCNA_01561 at 1679.977 kb on - strand, within CCNA_01561 at 1679.977 kb on - strand, within CCNA_01561 at 1680.025 kb on + strand, within CCNA_01561 at 1680.059 kb on - strand, within CCNA_01561 at 1680.075 kb on + strand, within CCNA_01561 at 1680.075 kb on + strand, within CCNA_01561 at 1680.075 kb on + strand, within CCNA_01561 at 1680.082 kb on + strand, within CCNA_01561 at 1680.178 kb on + strand at 1680.492 kb on + strand, within CCNA_01562 at 1680.533 kb on + strand, within CCNA_01562 at 1680.557 kb on + strand, within CCNA_01562 at 1680.565 kb on - strand, within CCNA_01562 at 1680.588 kb on + strand, within CCNA_01562 at 1680.588 kb on + strand, within CCNA_01562 at 1680.645 kb on + strand, within CCNA_01562 at 1680.674 kb on + strand, within CCNA_01562 at 1680.697 kb on + strand, within CCNA_01562 at 1680.705 kb on - strand, within CCNA_01562 at 1680.746 kb on + strand, within CCNA_01562 at 1680.748 kb on - strand, within CCNA_01562 at 1680.812 kb on + strand at 1680.812 kb on + strand at 1680.812 kb on + strand at 1680.812 kb on + strand at 1680.820 kb on - strand at 1680.911 kb on - strand at 1680.975 kb on + strand, within CCNA_01563 at 1680.975 kb on + strand, within CCNA_01563 at 1680.981 kb on + strand, within CCNA_01563 at 1681.000 kb on + strand, within CCNA_01563 at 1681.009 kb on - strand, within CCNA_01563 at 1681.021 kb on + strand, within CCNA_01563 at 1681.029 kb on + strand, within CCNA_01563 at 1681.029 kb on + strand, within CCNA_01563 at 1681.066 kb on - strand, within CCNA_01563 at 1681.075 kb on + strand, within CCNA_01563 at 1681.099 kb on + strand, within CCNA_01563 at 1681.125 kb on - strand, within CCNA_01563 at 1681.125 kb on - strand, within CCNA_01563
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hours of 100ug/mL streptomycin treatment after 3 hour phosphate starvation in M5G remove 1,678,817 - CCNA_01560 0.70 -3.8 1,678,819 + CCNA_01560 0.70 -1.1 1,678,819 + CCNA_01560 0.70 -2.9 1,678,819 + CCNA_01560 0.70 -2.8 1,678,819 + CCNA_01560 0.70 -1.5 1,678,901 - CCNA_01560 0.73 -1.5 1,678,921 + CCNA_01560 0.74 -3.6 1,678,921 + CCNA_01560 0.74 -3.9 1,678,921 + CCNA_01560 0.74 -3.2 1,678,974 - CCNA_01560 0.75 -4.3 1,678,989 + CCNA_01560 0.76 -1.4 1,678,989 + CCNA_01560 0.76 -1.9 1,678,989 + CCNA_01560 0.76 -3.0 1,678,993 - CCNA_01560 0.76 -3.5 1,678,998 - CCNA_01560 0.76 -3.9 1,678,998 - CCNA_01560 0.76 -2.0 1,678,998 - CCNA_01560 0.76 -3.6 1,679,001 + CCNA_01560 0.76 -4.4 1,679,009 + CCNA_01560 0.77 -2.0 1,679,017 - CCNA_01560 0.77 -3.5 1,679,041 + CCNA_01560 0.78 -3.5 1,679,127 + CCNA_01560 0.81 -3.1 1,679,127 + CCNA_01560 0.81 -2.8 1,679,142 + CCNA_01560 0.82 -3.2 1,679,153 + CCNA_01560 0.82 -2.6 1,679,153 + CCNA_01560 0.82 -2.5 1,679,161 + CCNA_01560 0.82 -2.4 1,679,161 - CCNA_01560 0.82 +0.5 1,679,161 - CCNA_01560 0.82 -1.0 1,679,202 + CCNA_01560 0.84 -3.9 1,679,205 - CCNA_01560 0.84 -3.2 1,679,288 + CCNA_01560 0.87 -2.6 1,679,310 + CCNA_01560 0.88 -4.1 1,679,314 - CCNA_01560 0.88 -2.9 1,679,317 + CCNA_01560 0.88 -2.0 1,679,370 + CCNA_01560 0.90 -2.0 1,679,370 + CCNA_01560 0.90 -3.4 1,679,378 - -2.4 1,679,378 - -2.7 1,679,378 - -1.8 1,679,397 + -4.3 1,679,409 + -0.1 1,679,426 + -2.5 1,679,426 + -3.3 1,679,434 - -2.0 1,679,460 + -3.1 1,679,469 - -2.4 1,679,494 + -3.0 1,679,495 - -4.3 1,679,513 - -2.4 1,679,520 + -2.8 1,679,858 - CCNA_01561 0.16 -0.7 1,679,909 - CCNA_01561 0.29 -0.8 1,679,955 - CCNA_01561 0.41 -0.1 1,679,955 - CCNA_01561 0.41 -0.1 1,679,977 - CCNA_01561 0.47 +0.8 1,679,977 - CCNA_01561 0.47 +0.8 1,680,025 + CCNA_01561 0.59 -0.3 1,680,059 - CCNA_01561 0.68 -2.7 1,680,075 + CCNA_01561 0.72 +0.1 1,680,075 + CCNA_01561 0.72 +0.1 1,680,075 + CCNA_01561 0.72 +0.9 1,680,082 + CCNA_01561 0.74 +0.0 1,680,178 + -2.1 1,680,492 + CCNA_01562 0.47 -3.3 1,680,533 + CCNA_01562 0.53 -1.6 1,680,557 + CCNA_01562 0.57 -5.1 1,680,565 - CCNA_01562 0.58 -0.8 1,680,588 + CCNA_01562 0.61 -2.0 1,680,588 + CCNA_01562 0.61 -3.1 1,680,645 + CCNA_01562 0.70 -4.0 1,680,674 + CCNA_01562 0.74 -4.3 1,680,697 + CCNA_01562 0.77 -4.7 1,680,705 - CCNA_01562 0.79 -3.0 1,680,746 + CCNA_01562 0.85 -3.0 1,680,748 - CCNA_01562 0.85 -1.7 1,680,812 + -3.2 1,680,812 + +0.5 1,680,812 + -1.6 1,680,812 + -4.0 1,680,820 - -3.3 1,680,911 - -0.1 1,680,975 + CCNA_01563 0.11 +0.7 1,680,975 + CCNA_01563 0.11 -0.5 1,680,981 + CCNA_01563 0.11 +0.0 1,681,000 + CCNA_01563 0.13 +0.7 1,681,009 - CCNA_01563 0.14 +0.3 1,681,021 + CCNA_01563 0.15 +0.4 1,681,029 + CCNA_01563 0.16 +0.1 1,681,029 + CCNA_01563 0.16 +1.0 1,681,066 - CCNA_01563 0.19 -0.6 1,681,075 + CCNA_01563 0.20 +0.8 1,681,099 + CCNA_01563 0.22 +0.4 1,681,125 - CCNA_01563 0.24 +1.5 1,681,125 - CCNA_01563 0.24 +2.1
Or see this region's nucleotide sequence