Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32000

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31990 and BPHYT_RS31995 are separated by 1 nucleotidesBPHYT_RS31995 and BPHYT_RS32000 overlap by 4 nucleotidesBPHYT_RS32000 and BPHYT_RS32005 are separated by 98 nucleotidesBPHYT_RS32005 and BPHYT_RS32010 are separated by 100 nucleotides BPHYT_RS31990: BPHYT_RS31990 - hypothetical protein, at 2,738,498 to 2,738,713 _RS31990 BPHYT_RS31995: BPHYT_RS31995 - general secretion pathway protein A, at 2,738,715 to 2,739,572 _RS31995 BPHYT_RS32000: BPHYT_RS32000 - integrase, at 2,739,569 to 2,741,005 _RS32000 BPHYT_RS32005: BPHYT_RS32005 - hypothetical protein, at 2,741,104 to 2,741,682 _RS32005 BPHYT_RS32010: BPHYT_RS32010 - DEAD/DEAH box helicase, at 2,741,783 to 2,744,374 _RS32010 Position (kb) 2739 2740 2741 2742Strain fitness (log2 ratio) -2 -1 0 1 2at 2741.875 kb on + strandat 2741.875 kb on + strandat 2741.875 kb on + strandat 2741.876 kb on - strandat 2741.974 kb on + strandat 2741.975 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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2,741,875 + +1.9
2,741,875 + +0.5
2,741,875 + -2.7
2,741,876 - -0.2
2,741,974 + -0.0
2,741,975 - +2.3

Or see this region's nucleotide sequence