Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23270

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23265 and BPHYT_RS23270 are separated by 41 nucleotidesBPHYT_RS23270 and BPHYT_RS23275 are separated by 81 nucleotides BPHYT_RS23265: BPHYT_RS23265 - methylcrotonoyl-CoA carboxylase, at 783,565 to 785,172 _RS23265 BPHYT_RS23270: BPHYT_RS23270 - enoyl-CoA hydratase, at 785,214 to 785,999 _RS23270 BPHYT_RS23275: BPHYT_RS23275 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 786,081 to 788,159 _RS23275 Position (kb) 785 786Strain fitness (log2 ratio) -1 0 1at 784.278 kb on + strand, within BPHYT_RS23265at 784.433 kb on - strand, within BPHYT_RS23265at 785.658 kb on + strand, within BPHYT_RS23270at 786.684 kb on + strand, within BPHYT_RS23275at 786.747 kb on + strand, within BPHYT_RS23275at 786.882 kb on + strand, within BPHYT_RS23275

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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784,278 + BPHYT_RS23265 0.44 +1.3
784,433 - BPHYT_RS23265 0.54 -1.3
785,658 + BPHYT_RS23270 0.56 +1.1
786,684 + BPHYT_RS23275 0.29 +1.6
786,747 + BPHYT_RS23275 0.32 +0.7
786,882 + BPHYT_RS23275 0.39 +0.7

Or see this region's nucleotide sequence