Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20185

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20180 and BPHYT_RS20185 are separated by 251 nucleotides BPHYT_RS20180: BPHYT_RS20180 - sugar ABC transporter substrate-binding protein, at 70,865 to 71,917 _RS20180 BPHYT_RS20185: BPHYT_RS20185 - sugar transferase, at 72,169 to 73,530 _RS20185 Position (kb) 72 73 74Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 71.182 kb on + strand, within BPHYT_RS20180at 71.251 kb on + strand, within BPHYT_RS20180at 72.843 kb on + strand, within BPHYT_RS20185at 73.255 kb on + strand, within BPHYT_RS20185at 73.874 kb on + strandat 74.305 kb on - strandat 74.312 kb on + strandat 74.313 kb on - strandat 74.320 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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71,182 + BPHYT_RS20180 0.30 -0.6
71,251 + BPHYT_RS20180 0.37 -1.1
72,843 + BPHYT_RS20185 0.49 +3.7
73,255 + BPHYT_RS20185 0.80 +0.4
73,874 + -0.6
74,305 - +0.3
74,312 + +0.3
74,313 - -0.5
74,320 + -3.9

Or see this region's nucleotide sequence