Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS17005

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16995 and BPHYT_RS17000 are separated by 10 nucleotidesBPHYT_RS17000 and BPHYT_RS17005 are separated by 194 nucleotidesBPHYT_RS17005 and BPHYT_RS17010 are separated by 67 nucleotidesBPHYT_RS17010 and BPHYT_RS17015 are separated by 130 nucleotides BPHYT_RS16995: BPHYT_RS16995 - UDP-N-acetylmuramate--alanine ligase, at 3,854,687 to 3,855,340 _RS16995 BPHYT_RS17000: BPHYT_RS17000 - redoxin, at 3,855,351 to 3,855,902 _RS17000 BPHYT_RS17005: BPHYT_RS17005 - 3-dehydroquinate dehydratase, at 3,856,097 to 3,856,552 _RS17005 BPHYT_RS17010: BPHYT_RS17010 - acetyl-CoA carboxylase, at 3,856,620 to 3,857,090 _RS17010 BPHYT_RS17015: BPHYT_RS17015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 3,857,221 to 3,858,588 _RS17015 Position (kb) 3856 3857Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 3855.099 kb on - strand, within BPHYT_RS16995at 3855.099 kb on - strand, within BPHYT_RS16995at 3855.138 kb on - strand, within BPHYT_RS16995at 3855.218 kb on + strand, within BPHYT_RS16995at 3855.328 kb on + strandat 3855.329 kb on - strandat 3855.329 kb on - strandat 3855.329 kb on - strandat 3855.339 kb on - strandat 3855.339 kb on - strandat 3855.339 kb on - strandat 3855.356 kb on + strandat 3855.357 kb on - strandat 3855.741 kb on + strand, within BPHYT_RS17000

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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3,855,099 - BPHYT_RS16995 0.63 +0.5
3,855,099 - BPHYT_RS16995 0.63 +0.6
3,855,138 - BPHYT_RS16995 0.69 -1.4
3,855,218 + BPHYT_RS16995 0.81 -0.7
3,855,328 + -1.4
3,855,329 - +0.5
3,855,329 - -0.5
3,855,329 - -0.3
3,855,339 - +4.8
3,855,339 - +0.6
3,855,339 - +1.4
3,855,356 + -0.6
3,855,357 - +0.6
3,855,741 + BPHYT_RS17000 0.71 -1.7

Or see this region's nucleotide sequence