Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16995 and BPHYT_RS17000 are separated by 10 nucleotides BPHYT_RS17000 and BPHYT_RS17005 are separated by 194 nucleotides BPHYT_RS17005 and BPHYT_RS17010 are separated by 67 nucleotides BPHYT_RS17010 and BPHYT_RS17015 are separated by 130 nucleotides
BPHYT_RS16995: BPHYT_RS16995 - UDP-N-acetylmuramate--alanine ligase, at 3,854,687 to 3,855,340
_RS16995
BPHYT_RS17000: BPHYT_RS17000 - redoxin, at 3,855,351 to 3,855,902
_RS17000
BPHYT_RS17005: BPHYT_RS17005 - 3-dehydroquinate dehydratase, at 3,856,097 to 3,856,552
_RS17005
BPHYT_RS17010: BPHYT_RS17010 - acetyl-CoA carboxylase, at 3,856,620 to 3,857,090
_RS17010
BPHYT_RS17015: BPHYT_RS17015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 3,857,221 to 3,858,588
_RS17015
Position (kb)
3856
3857 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3855.099 kb on - strand, within BPHYT_RS16995 at 3855.099 kb on - strand, within BPHYT_RS16995 at 3855.138 kb on - strand, within BPHYT_RS16995 at 3855.218 kb on + strand, within BPHYT_RS16995 at 3855.328 kb on + strand at 3855.329 kb on - strand at 3855.329 kb on - strand at 3855.329 kb on - strand at 3855.339 kb on - strand at 3855.339 kb on - strand at 3855.339 kb on - strand at 3855.356 kb on + strand at 3855.357 kb on - strand at 3855.741 kb on + strand, within BPHYT_RS17000
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 3,855,099 - BPHYT_RS16995 0.63 +0.5 3,855,099 - BPHYT_RS16995 0.63 +0.6 3,855,138 - BPHYT_RS16995 0.69 -1.4 3,855,218 + BPHYT_RS16995 0.81 -0.7 3,855,328 + -1.4 3,855,329 - +0.5 3,855,329 - -0.5 3,855,329 - -0.3 3,855,339 - +4.8 3,855,339 - +0.6 3,855,339 - +1.4 3,855,356 + -0.6 3,855,357 - +0.6 3,855,741 + BPHYT_RS17000 0.71 -1.7
Or see this region's nucleotide sequence