Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15305

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15290 and BPHYT_RS15295 are separated by 79 nucleotidesBPHYT_RS15295 and BPHYT_RS15300 are separated by 113 nucleotidesBPHYT_RS15300 and BPHYT_RS15305 are separated by 108 nucleotidesBPHYT_RS15305 and BPHYT_RS15310 are separated by 150 nucleotidesBPHYT_RS15310 and BPHYT_RS15315 are separated by 60 nucleotides BPHYT_RS15290: BPHYT_RS15290 - tRNA-Arg, at 3,475,597 to 3,475,673 _RS15290 BPHYT_RS15295: BPHYT_RS15295 - tRNA-Arg, at 3,475,753 to 3,475,829 _RS15295 BPHYT_RS15300: BPHYT_RS15300 - hypothetical protein, at 3,475,943 to 3,476,452 _RS15300 BPHYT_RS15305: BPHYT_RS15305 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 3,476,561 to 3,478,918 _RS15305 BPHYT_RS15310: BPHYT_RS15310 - DNA-directed RNA polymerase subunit omega, at 3,479,069 to 3,479,272 _RS15310 BPHYT_RS15315: BPHYT_RS15315 - guanylate kinase, at 3,479,333 to 3,480,013 _RS15315 Position (kb) 3476 3477 3478 3479Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3475.604 kb on + strandat 3475.622 kb on + strand, within BPHYT_RS15290at 3475.622 kb on + strand, within BPHYT_RS15290at 3475.622 kb on + strand, within BPHYT_RS15290at 3475.676 kb on + strandat 3475.676 kb on + strandat 3475.713 kb on + strandat 3475.733 kb on - strandat 3475.747 kb on + strandat 3475.832 kb on + strandat 3476.302 kb on + strand, within BPHYT_RS15300at 3476.303 kb on - strand, within BPHYT_RS15300at 3476.373 kb on + strand, within BPHYT_RS15300at 3476.565 kb on + strandat 3476.565 kb on + strandat 3476.565 kb on + strandat 3476.777 kb on - strandat 3476.798 kb on - strand, within BPHYT_RS15305at 3476.798 kb on - strand, within BPHYT_RS15305at 3476.798 kb on - strand, within BPHYT_RS15305at 3476.985 kb on + strand, within BPHYT_RS15305at 3476.986 kb on - strand, within BPHYT_RS15305at 3476.986 kb on - strand, within BPHYT_RS15305at 3476.986 kb on - strand, within BPHYT_RS15305at 3476.986 kb on - strand, within BPHYT_RS15305at 3476.986 kb on - strand, within BPHYT_RS15305at 3477.061 kb on + strand, within BPHYT_RS15305at 3477.421 kb on - strand, within BPHYT_RS15305at 3477.537 kb on + strand, within BPHYT_RS15305at 3477.603 kb on + strand, within BPHYT_RS15305at 3477.604 kb on - strand, within BPHYT_RS15305at 3477.604 kb on - strand, within BPHYT_RS15305at 3477.604 kb on - strand, within BPHYT_RS15305at 3477.604 kb on - strand, within BPHYT_RS15305at 3478.442 kb on - strand, within BPHYT_RS15305at 3478.729 kb on - strandat 3478.972 kb on - strandat 3478.972 kb on - strandat 3478.972 kb on - strandat 3478.972 kb on - strandat 3478.972 kb on - strandat 3478.975 kb on + strandat 3478.975 kb on + strandat 3478.976 kb on - strandat 3479.309 kb on + strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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3,475,604 + -0.9
3,475,622 + BPHYT_RS15290 0.32 +0.1
3,475,622 + BPHYT_RS15290 0.32 +1.4
3,475,622 + BPHYT_RS15290 0.32 -1.4
3,475,676 + +0.8
3,475,676 + -0.5
3,475,713 + +0.5
3,475,733 - -1.6
3,475,747 + +1.3
3,475,832 + +0.1
3,476,302 + BPHYT_RS15300 0.70 +2.1
3,476,303 - BPHYT_RS15300 0.71 +0.3
3,476,373 + BPHYT_RS15300 0.84 -1.0
3,476,565 + -0.8
3,476,565 + -2.0
3,476,565 + -0.9
3,476,777 - -1.6
3,476,798 - BPHYT_RS15305 0.10 -2.4
3,476,798 - BPHYT_RS15305 0.10 -2.4
3,476,798 - BPHYT_RS15305 0.10 -2.5
3,476,985 + BPHYT_RS15305 0.18 -0.5
3,476,986 - BPHYT_RS15305 0.18 -0.9
3,476,986 - BPHYT_RS15305 0.18 -0.7
3,476,986 - BPHYT_RS15305 0.18 -1.4
3,476,986 - BPHYT_RS15305 0.18 -1.3
3,476,986 - BPHYT_RS15305 0.18 -0.1
3,477,061 + BPHYT_RS15305 0.21 -1.8
3,477,421 - BPHYT_RS15305 0.36 -0.3
3,477,537 + BPHYT_RS15305 0.41 +3.0
3,477,603 + BPHYT_RS15305 0.44 -3.4
3,477,604 - BPHYT_RS15305 0.44 -1.7
3,477,604 - BPHYT_RS15305 0.44 -1.2
3,477,604 - BPHYT_RS15305 0.44 -1.4
3,477,604 - BPHYT_RS15305 0.44 -1.0
3,478,442 - BPHYT_RS15305 0.80 -1.6
3,478,729 - -1.4
3,478,972 - -0.1
3,478,972 - -0.3
3,478,972 - -1.5
3,478,972 - +0.6
3,478,972 - -0.1
3,478,975 + -0.6
3,478,975 + -0.6
3,478,976 - -1.6
3,479,309 + -0.2
3,479,310 - +2.1
3,479,310 - +2.4
3,479,310 - +2.4
3,479,310 - +1.7
3,479,310 - +2.2
3,479,310 - +2.2
3,479,310 - +1.6

Or see this region's nucleotide sequence