Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4595 and Pf6N2E2_4596 are separated by 25 nucleotides Pf6N2E2_4596 and Pf6N2E2_4597 are separated by 7 nucleotides Pf6N2E2_4597 and Pf6N2E2_4598 are separated by 309 nucleotides Pf6N2E2_4598 and Pf6N2E2_4599 are separated by 5 nucleotides
Pf6N2E2_4595 - FIG003551: hypothetical protein, at 5,322,906 to 5,323,340
_4595
Pf6N2E2_4596 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 5,323,366 to 5,323,986
_4596
Pf6N2E2_4597 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 5,323,994 to 5,325,085
_4597
Pf6N2E2_4598 - COG1872, at 5,325,395 to 5,325,688
_4598
Pf6N2E2_4599 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 5,325,694 to 5,326,284
_4599
Position (kb) 5323
5324
5325
5326 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.024 kb on + strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.242 kb on - strand, within Pf6N2E2_4595 at 5323.382 kb on + strand at 5323.383 kb on - strand at 5323.390 kb on - strand at 5323.413 kb on - strand at 5323.420 kb on + strand at 5323.420 kb on + strand at 5323.420 kb on + strand at 5323.429 kb on + strand, within Pf6N2E2_4596 at 5323.437 kb on + strand, within Pf6N2E2_4596 at 5323.438 kb on - strand, within Pf6N2E2_4596 at 5323.444 kb on + strand, within Pf6N2E2_4596 at 5323.444 kb on + strand, within Pf6N2E2_4596 at 5323.444 kb on + strand, within Pf6N2E2_4596 at 5323.444 kb on + strand, within Pf6N2E2_4596 at 5323.444 kb on + strand, within Pf6N2E2_4596 at 5323.444 kb on + strand, within Pf6N2E2_4596 at 5323.445 kb on - strand, within Pf6N2E2_4596 at 5323.445 kb on - strand, within Pf6N2E2_4596 at 5323.445 kb on - strand, within Pf6N2E2_4596 at 5323.445 kb on - strand, within Pf6N2E2_4596 at 5323.445 kb on - strand, within Pf6N2E2_4596 at 5323.445 kb on - strand, within Pf6N2E2_4596 at 5323.445 kb on - strand, within Pf6N2E2_4596 at 5323.571 kb on + strand, within Pf6N2E2_4596 at 5323.571 kb on + strand, within Pf6N2E2_4596 at 5323.571 kb on + strand, within Pf6N2E2_4596 at 5323.571 kb on + strand, within Pf6N2E2_4596 at 5323.571 kb on + strand, within Pf6N2E2_4596 at 5323.571 kb on + strand, within Pf6N2E2_4596 at 5323.571 kb on + strand, within Pf6N2E2_4596 at 5323.572 kb on - strand, within Pf6N2E2_4596 at 5323.580 kb on + strand, within Pf6N2E2_4596 at 5323.581 kb on - strand, within Pf6N2E2_4596 at 5323.625 kb on + strand, within Pf6N2E2_4596 at 5323.626 kb on - strand, within Pf6N2E2_4596 at 5324.004 kb on - strand at 5324.005 kb on + strand at 5324.005 kb on + strand at 5324.005 kb on + strand at 5324.005 kb on + strand at 5324.005 kb on + strand at 5324.005 kb on + strand at 5324.006 kb on - strand at 5324.006 kb on - strand at 5324.006 kb on - strand at 5324.016 kb on + strand at 5324.016 kb on + strand at 5324.016 kb on + strand at 5324.016 kb on + strand at 5324.017 kb on - strand at 5324.017 kb on - strand at 5324.017 kb on - strand at 5324.257 kb on - strand, within Pf6N2E2_4597 at 5324.283 kb on + strand, within Pf6N2E2_4597 at 5324.283 kb on + strand, within Pf6N2E2_4597 at 5324.283 kb on + strand, within Pf6N2E2_4597 at 5324.283 kb on + strand, within Pf6N2E2_4597 at 5324.283 kb on + strand, within Pf6N2E2_4597 at 5324.283 kb on + strand, within Pf6N2E2_4597 at 5324.284 kb on - strand, within Pf6N2E2_4597 at 5324.284 kb on - strand, within Pf6N2E2_4597 at 5324.284 kb on - strand, within Pf6N2E2_4597 at 5324.284 kb on - strand, within Pf6N2E2_4597 at 5324.335 kb on - strand, within Pf6N2E2_4597 at 5324.370 kb on + strand, within Pf6N2E2_4597 at 5324.370 kb on + strand, within Pf6N2E2_4597 at 5324.370 kb on + strand, within Pf6N2E2_4597 at 5324.371 kb on - strand, within Pf6N2E2_4597 at 5324.371 kb on - strand, within Pf6N2E2_4597 at 5324.433 kb on + strand, within Pf6N2E2_4597 at 5324.433 kb on + strand, within Pf6N2E2_4597 at 5324.433 kb on + strand, within Pf6N2E2_4597 at 5324.434 kb on - strand, within Pf6N2E2_4597 at 5324.434 kb on - strand, within Pf6N2E2_4597 at 5324.434 kb on - strand, within Pf6N2E2_4597 at 5325.009 kb on + strand at 5325.018 kb on + strand at 5325.018 kb on + strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.030 kb on + strand at 5325.031 kb on - strand at 5325.031 kb on - strand at 5325.031 kb on - strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.162 kb on - strand at 5325.167 kb on + strand at 5325.168 kb on - strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.319 kb on - strand at 5325.319 kb on - strand at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.681 kb on + strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.828 kb on + strand, within Pf6N2E2_4599 at 5325.828 kb on + strand, within Pf6N2E2_4599 at 5325.829 kb on - strand, within Pf6N2E2_4599 at 5326.020 kb on - strand, within Pf6N2E2_4599
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 5,323,021 + Pf6N2E2_4595 0.26 -0.9 5,323,021 + Pf6N2E2_4595 0.26 -0.0 5,323,021 + Pf6N2E2_4595 0.26 +0.8 5,323,021 + Pf6N2E2_4595 0.26 +0.6 5,323,021 + Pf6N2E2_4595 0.26 -0.4 5,323,021 + Pf6N2E2_4595 0.26 +1.1 5,323,022 - Pf6N2E2_4595 0.27 +0.2 5,323,022 - Pf6N2E2_4595 0.27 +0.9 5,323,022 - Pf6N2E2_4595 0.27 -0.1 5,323,022 - Pf6N2E2_4595 0.27 +0.6 5,323,024 + Pf6N2E2_4595 0.27 -0.5 5,323,025 - Pf6N2E2_4595 0.27 +1.5 5,323,025 - Pf6N2E2_4595 0.27 +0.3 5,323,025 - Pf6N2E2_4595 0.27 -0.5 5,323,025 - Pf6N2E2_4595 0.27 -1.9 5,323,228 + Pf6N2E2_4595 0.74 -0.2 5,323,228 + Pf6N2E2_4595 0.74 +0.1 5,323,228 + Pf6N2E2_4595 0.74 +0.8 5,323,228 + Pf6N2E2_4595 0.74 -0.1 5,323,228 + Pf6N2E2_4595 0.74 +0.1 5,323,228 + Pf6N2E2_4595 0.74 -0.2 5,323,229 - Pf6N2E2_4595 0.74 -1.1 5,323,229 - Pf6N2E2_4595 0.74 +0.8 5,323,229 - Pf6N2E2_4595 0.74 +0.3 5,323,229 - Pf6N2E2_4595 0.74 +0.2 5,323,242 - Pf6N2E2_4595 0.77 -1.1 5,323,382 + +0.0 5,323,383 - +0.3 5,323,390 - -0.8 5,323,413 - -3.6 5,323,420 + -4.3 5,323,420 + -3.4 5,323,420 + -4.3 5,323,429 + Pf6N2E2_4596 0.10 -2.9 5,323,437 + Pf6N2E2_4596 0.11 -2.2 5,323,438 - Pf6N2E2_4596 0.12 -2.2 5,323,444 + Pf6N2E2_4596 0.13 -3.6 5,323,444 + Pf6N2E2_4596 0.13 -2.3 5,323,444 + Pf6N2E2_4596 0.13 -2.5 5,323,444 + Pf6N2E2_4596 0.13 -3.6 5,323,444 + Pf6N2E2_4596 0.13 -1.6 5,323,444 + Pf6N2E2_4596 0.13 -3.1 5,323,445 - Pf6N2E2_4596 0.13 -3.3 5,323,445 - Pf6N2E2_4596 0.13 -2.7 5,323,445 - Pf6N2E2_4596 0.13 -4.1 5,323,445 - Pf6N2E2_4596 0.13 -3.9 5,323,445 - Pf6N2E2_4596 0.13 -3.4 5,323,445 - Pf6N2E2_4596 0.13 -3.0 5,323,445 - Pf6N2E2_4596 0.13 -3.4 5,323,571 + Pf6N2E2_4596 0.33 -3.0 5,323,571 + Pf6N2E2_4596 0.33 -2.0 5,323,571 + Pf6N2E2_4596 0.33 -2.4 5,323,571 + Pf6N2E2_4596 0.33 -3.5 5,323,571 + Pf6N2E2_4596 0.33 -3.6 5,323,571 + Pf6N2E2_4596 0.33 -4.2 5,323,571 + Pf6N2E2_4596 0.33 -2.0 5,323,572 - Pf6N2E2_4596 0.33 -2.8 5,323,580 + Pf6N2E2_4596 0.34 -3.1 5,323,581 - Pf6N2E2_4596 0.35 -2.6 5,323,625 + Pf6N2E2_4596 0.42 -2.2 5,323,626 - Pf6N2E2_4596 0.42 -3.0 5,324,004 - +0.6 5,324,005 + -2.9 5,324,005 + -2.4 5,324,005 + -3.3 5,324,005 + -4.3 5,324,005 + -1.6 5,324,005 + -2.7 5,324,006 - -3.7 5,324,006 - -3.9 5,324,006 - -2.9 5,324,016 + -4.5 5,324,016 + -3.0 5,324,016 + -0.7 5,324,016 + -3.7 5,324,017 - -3.5 5,324,017 - -2.7 5,324,017 - -3.2 5,324,257 - Pf6N2E2_4597 0.24 -3.4 5,324,283 + Pf6N2E2_4597 0.26 -3.4 5,324,283 + Pf6N2E2_4597 0.26 -4.1 5,324,283 + Pf6N2E2_4597 0.26 -4.2 5,324,283 + Pf6N2E2_4597 0.26 -3.6 5,324,283 + Pf6N2E2_4597 0.26 -3.9 5,324,283 + Pf6N2E2_4597 0.26 -1.0 5,324,284 - Pf6N2E2_4597 0.27 -2.8 5,324,284 - Pf6N2E2_4597 0.27 -3.6 5,324,284 - Pf6N2E2_4597 0.27 -2.9 5,324,284 - Pf6N2E2_4597 0.27 -4.5 5,324,335 - Pf6N2E2_4597 0.31 -2.8 5,324,370 + Pf6N2E2_4597 0.34 -2.0 5,324,370 + Pf6N2E2_4597 0.34 -2.7 5,324,370 + Pf6N2E2_4597 0.34 -3.0 5,324,371 - Pf6N2E2_4597 0.35 -3.1 5,324,371 - Pf6N2E2_4597 0.35 -3.4 5,324,433 + Pf6N2E2_4597 0.40 -3.9 5,324,433 + Pf6N2E2_4597 0.40 -3.3 5,324,433 + Pf6N2E2_4597 0.40 -3.5 5,324,434 - Pf6N2E2_4597 0.40 -4.1 5,324,434 - Pf6N2E2_4597 0.40 -4.0 5,324,434 - Pf6N2E2_4597 0.40 -3.2 5,325,009 + -2.1 5,325,018 + -3.6 5,325,018 + -1.2 5,325,019 - -2.9 5,325,019 - -3.1 5,325,019 - -3.5 5,325,019 - -2.9 5,325,019 - -4.1 5,325,030 + -3.4 5,325,031 - -4.5 5,325,031 - -4.2 5,325,031 - -3.5 5,325,161 + -3.7 5,325,161 + -0.7 5,325,161 + -3.4 5,325,161 + -3.2 5,325,161 + -2.4 5,325,161 + -3.1 5,325,161 + -2.9 5,325,161 + -2.4 5,325,161 + -3.0 5,325,162 - -2.4 5,325,167 + -1.2 5,325,168 - -0.5 5,325,314 + +1.5 5,325,314 + -0.0 5,325,314 + -0.0 5,325,314 + +1.1 5,325,314 + +0.2 5,325,314 + -0.9 5,325,314 + -0.3 5,325,315 - +0.8 5,325,315 - +0.7 5,325,315 - +0.6 5,325,315 - -1.7 5,325,315 - +0.2 5,325,315 - +1.0 5,325,315 - -0.5 5,325,315 - -0.9 5,325,315 - -0.2 5,325,318 + +0.0 5,325,318 + +0.9 5,325,318 + -1.3 5,325,318 + -0.9 5,325,319 - +0.3 5,325,319 - -0.7 5,325,446 + Pf6N2E2_4598 0.17 -0.8 5,325,446 + Pf6N2E2_4598 0.17 +0.8 5,325,446 + Pf6N2E2_4598 0.17 +0.4 5,325,446 + Pf6N2E2_4598 0.17 -0.1 5,325,446 + Pf6N2E2_4598 0.17 -0.1 5,325,447 - Pf6N2E2_4598 0.18 -0.7 5,325,447 - Pf6N2E2_4598 0.18 +0.4 5,325,447 - Pf6N2E2_4598 0.18 +0.5 5,325,447 - Pf6N2E2_4598 0.18 -0.5 5,325,447 - Pf6N2E2_4598 0.18 +0.7 5,325,681 + +0.8 5,325,682 - -0.3 5,325,682 - -1.6 5,325,682 - +0.2 5,325,682 - +0.4 5,325,682 - +0.1 5,325,682 - +0.4 5,325,682 - +0.5 5,325,828 + Pf6N2E2_4599 0.23 +0.6 5,325,828 + Pf6N2E2_4599 0.23 -0.4 5,325,829 - Pf6N2E2_4599 0.23 -0.0 5,326,020 - Pf6N2E2_4599 0.55 +1.0
Or see this region's nucleotide sequence