Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28615

Experiment: Phenylacetic 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28610 and BPHYT_RS28615 are separated by 186 nucleotidesBPHYT_RS28615 and BPHYT_RS28620 are separated by 5 nucleotides BPHYT_RS28610: BPHYT_RS28610 - beta-lactamase, at 1,971,213 to 1,973,081 _RS28610 BPHYT_RS28615: BPHYT_RS28615 - 6-carboxyhexanoate--CoA ligase, at 1,973,268 to 1,975,400 _RS28615 BPHYT_RS28620: BPHYT_RS28620 - enoyl-CoA hydratase, at 1,975,406 to 1,976,188 _RS28620 Position (kb) 1973 1974 1975 1976Strain fitness (log2 ratio) -1 0 1 2 3at 1972.477 kb on - strand, within BPHYT_RS28610at 1972.683 kb on + strand, within BPHYT_RS28610at 1973.010 kb on + strandat 1973.166 kb on - strandat 1975.459 kb on + strandat 1975.459 kb on + strandat 1975.460 kb on - strandat 1975.731 kb on + strand, within BPHYT_RS28620at 1975.770 kb on + strand, within BPHYT_RS28620at 1975.771 kb on - strand, within BPHYT_RS28620at 1975.771 kb on - strand, within BPHYT_RS28620at 1976.195 kb on - strandat 1976.307 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phenylacetic 5 mM (C)
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1,972,477 - BPHYT_RS28610 0.68 -0.3
1,972,683 + BPHYT_RS28610 0.79 +0.5
1,973,010 + -0.3
1,973,166 - -0.6
1,975,459 + -0.8
1,975,459 + +0.0
1,975,460 - +0.2
1,975,731 + BPHYT_RS28620 0.42 -0.3
1,975,770 + BPHYT_RS28620 0.46 -1.3
1,975,771 - BPHYT_RS28620 0.47 +1.8
1,975,771 - BPHYT_RS28620 0.47 +1.8
1,976,195 - +3.5
1,976,307 + +0.4

Or see this region's nucleotide sequence