Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4635 and BT4636 are separated by 82 nucleotides BT4636 and BT4637 are separated by 251 nucleotides BT4637 and BT4638 are separated by 16 nucleotides BT4638 and BT4639 are separated by 79 nucleotides
BT4635: BT4635 - putative anti-sigma factor (NCBI ptt file), at 6,070,725 to 6,071,939
BT4635
BT4636: BT4636 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 6,072,022 to 6,072,615
BT4636
BT4637: BT4637 - putative inorganic phosphate transporter (NCBI ptt file), at 6,072,867 to 6,073,886
BT4637
BT4638: BT4638 - conserved hypothetical protein (NCBI ptt file), at 6,073,903 to 6,074,550
BT4638
BT4639: BT4639 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 6,074,630 to 6,075,271
BT4639
Position (kb)
6072
6073
6074 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6071.895 kb on + strand at 6071.896 kb on - strand at 6071.918 kb on + strand at 6071.919 kb on - strand at 6071.920 kb on + strand at 6071.920 kb on + strand at 6071.954 kb on + strand at 6071.954 kb on + strand at 6071.955 kb on - strand at 6071.960 kb on - strand at 6072.029 kb on - strand at 6072.052 kb on + strand at 6072.077 kb on + strand at 6072.089 kb on - strand, within BT4636 at 6072.117 kb on + strand, within BT4636 at 6072.156 kb on + strand, within BT4636 at 6072.156 kb on + strand, within BT4636 at 6072.164 kb on + strand, within BT4636 at 6072.237 kb on + strand, within BT4636 at 6072.240 kb on + strand, within BT4636 at 6072.329 kb on + strand, within BT4636 at 6072.365 kb on + strand, within BT4636 at 6072.438 kb on + strand, within BT4636 at 6072.440 kb on + strand, within BT4636 at 6072.441 kb on - strand, within BT4636 at 6072.509 kb on + strand, within BT4636 at 6072.530 kb on + strand, within BT4636 at 6072.530 kb on + strand, within BT4636 at 6072.531 kb on - strand, within BT4636 at 6072.540 kb on - strand, within BT4636 at 6072.551 kb on + strand, within BT4636 at 6072.551 kb on + strand, within BT4636 at 6072.552 kb on - strand, within BT4636 at 6072.552 kb on - strand, within BT4636 at 6072.591 kb on + strand at 6072.616 kb on - strand at 6072.621 kb on + strand at 6072.729 kb on - strand at 6072.750 kb on - strand at 6072.761 kb on + strand at 6072.781 kb on - strand at 6072.781 kb on - strand at 6072.801 kb on + strand at 6072.802 kb on - strand at 6072.868 kb on + strand at 6072.888 kb on + strand at 6072.899 kb on + strand at 6072.933 kb on - strand at 6072.960 kb on - strand at 6073.029 kb on - strand, within BT4637 at 6073.029 kb on - strand, within BT4637 at 6073.029 kb on - strand, within BT4637 at 6073.040 kb on + strand, within BT4637 at 6073.041 kb on - strand, within BT4637 at 6073.069 kb on + strand, within BT4637 at 6073.103 kb on + strand, within BT4637 at 6073.103 kb on + strand, within BT4637 at 6073.103 kb on + strand, within BT4637 at 6073.125 kb on - strand, within BT4637 at 6073.239 kb on + strand, within BT4637 at 6073.240 kb on - strand, within BT4637 at 6073.272 kb on + strand, within BT4637 at 6073.398 kb on - strand, within BT4637 at 6073.409 kb on + strand, within BT4637 at 6073.410 kb on - strand, within BT4637 at 6073.411 kb on + strand, within BT4637 at 6073.442 kb on + strand, within BT4637 at 6073.509 kb on - strand, within BT4637 at 6073.555 kb on - strand, within BT4637 at 6073.557 kb on - strand, within BT4637 at 6073.643 kb on + strand, within BT4637 at 6073.644 kb on - strand, within BT4637 at 6073.692 kb on + strand, within BT4637 at 6073.708 kb on - strand, within BT4637 at 6073.839 kb on - strand at 6073.857 kb on + strand at 6073.858 kb on - strand at 6073.858 kb on - strand at 6073.860 kb on + strand at 6073.861 kb on - strand at 6073.904 kb on + strand at 6073.911 kb on - strand at 6073.998 kb on + strand, within BT4638 at 6074.086 kb on + strand, within BT4638 at 6074.086 kb on + strand, within BT4638 at 6074.087 kb on - strand, within BT4638 at 6074.154 kb on + strand, within BT4638 at 6074.233 kb on + strand, within BT4638 at 6074.239 kb on - strand, within BT4638 at 6074.326 kb on - strand, within BT4638 at 6074.402 kb on + strand, within BT4638 at 6074.483 kb on - strand, within BT4638 at 6074.524 kb on - strand at 6074.585 kb on + strand at 6074.667 kb on - strand at 6074.698 kb on + strand, within BT4639 at 6074.754 kb on + strand, within BT4639 at 6074.763 kb on + strand, within BT4639 at 6074.795 kb on + strand, within BT4639
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 6,071,895 + -0.2 6,071,896 - +2.2 6,071,918 + -2.0 6,071,919 - +0.9 6,071,920 + +1.9 6,071,920 + -0.1 6,071,954 + -1.1 6,071,954 + -1.7 6,071,955 - -2.1 6,071,960 - -1.9 6,072,029 - -2.3 6,072,052 + +0.3 6,072,077 + +0.1 6,072,089 - BT4636 0.11 -0.1 6,072,117 + BT4636 0.16 +1.2 6,072,156 + BT4636 0.23 -0.1 6,072,156 + BT4636 0.23 -0.1 6,072,164 + BT4636 0.24 -2.3 6,072,237 + BT4636 0.36 -1.5 6,072,240 + BT4636 0.37 -1.1 6,072,329 + BT4636 0.52 -0.3 6,072,365 + BT4636 0.58 -0.1 6,072,438 + BT4636 0.70 +1.5 6,072,440 + BT4636 0.70 +1.3 6,072,441 - BT4636 0.71 +1.5 6,072,509 + BT4636 0.82 -1.7 6,072,530 + BT4636 0.86 -4.0 6,072,530 + BT4636 0.86 +2.2 6,072,531 - BT4636 0.86 -0.9 6,072,540 - BT4636 0.87 -0.6 6,072,551 + BT4636 0.89 +2.9 6,072,551 + BT4636 0.89 +0.6 6,072,552 - BT4636 0.89 +0.9 6,072,552 - BT4636 0.89 +0.9 6,072,591 + +0.0 6,072,616 - -2.3 6,072,621 + +0.7 6,072,729 - +0.9 6,072,750 - -1.6 6,072,761 + +2.1 6,072,781 - +1.5 6,072,781 - -0.9 6,072,801 + -0.2 6,072,802 - -0.4 6,072,868 + +0.2 6,072,888 + -3.8 6,072,899 + -0.8 6,072,933 - +0.9 6,072,960 - +1.5 6,073,029 - BT4637 0.16 +3.0 6,073,029 - BT4637 0.16 +0.3 6,073,029 - BT4637 0.16 -1.7 6,073,040 + BT4637 0.17 -0.4 6,073,041 - BT4637 0.17 -1.5 6,073,069 + BT4637 0.20 +0.1 6,073,103 + BT4637 0.23 -1.5 6,073,103 + BT4637 0.23 +0.1 6,073,103 + BT4637 0.23 +0.9 6,073,125 - BT4637 0.25 -4.0 6,073,239 + BT4637 0.36 -0.5 6,073,240 - BT4637 0.37 -1.5 6,073,272 + BT4637 0.40 +1.9 6,073,398 - BT4637 0.52 +0.6 6,073,409 + BT4637 0.53 -0.2 6,073,410 - BT4637 0.53 +0.9 6,073,411 + BT4637 0.53 +0.0 6,073,442 + BT4637 0.56 +0.5 6,073,509 - BT4637 0.63 -2.4 6,073,555 - BT4637 0.67 -1.4 6,073,557 - BT4637 0.68 +2.3 6,073,643 + BT4637 0.76 +2.0 6,073,644 - BT4637 0.76 -1.2 6,073,692 + BT4637 0.81 +2.6 6,073,708 - BT4637 0.82 -2.1 6,073,839 - -0.1 6,073,857 + +0.8 6,073,858 - -2.0 6,073,858 - +0.2 6,073,860 + -0.1 6,073,861 - -1.7 6,073,904 + -2.1 6,073,911 - +2.5 6,073,998 + BT4638 0.15 -0.7 6,074,086 + BT4638 0.28 +0.2 6,074,086 + BT4638 0.28 -1.1 6,074,087 - BT4638 0.28 -1.1 6,074,154 + BT4638 0.39 -0.8 6,074,233 + BT4638 0.51 -1.5 6,074,239 - BT4638 0.52 -1.7 6,074,326 - BT4638 0.65 -0.7 6,074,402 + BT4638 0.77 +2.5 6,074,483 - BT4638 0.90 +2.7 6,074,524 - -1.1 6,074,585 + -0.0 6,074,667 - -1.1 6,074,698 + BT4639 0.11 +0.3 6,074,754 + BT4639 0.19 -2.9 6,074,763 + BT4639 0.21 -2.1 6,074,795 + BT4639 0.26 -0.6
Or see this region's nucleotide sequence