Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4461

Experiment: BHIS with Chlorpromazine 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4460 and BT4461 overlap by 4 nucleotidesBT4461 and BT4462 are separated by 5 nucleotidesBT4462 and BT4463 are separated by 25 nucleotides BT4460: BT4460 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,870,614 to 5,871,963 BT4460 BT4461: BT4461 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,871,960 to 5,872,532 BT4461 BT4462: BT4462 - conserved hypothetical protein (NCBI ptt file), at 5,872,538 to 5,873,191 BT4462 BT4463: BT4463 - phosphatidate cytidylyltransferase (NCBI ptt file), at 5,873,217 to 5,874,173 BT4463 Position (kb) 5871 5872 5873Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 5871.010 kb on + strand, within BT4460at 5871.038 kb on - strand, within BT4460at 5871.040 kb on + strand, within BT4460at 5871.041 kb on - strand, within BT4460at 5871.079 kb on + strand, within BT4460at 5871.080 kb on - strand, within BT4460at 5871.080 kb on - strand, within BT4460at 5871.087 kb on + strand, within BT4460at 5871.087 kb on + strand, within BT4460at 5871.111 kb on + strand, within BT4460at 5871.111 kb on + strand, within BT4460at 5871.133 kb on - strand, within BT4460at 5871.135 kb on - strand, within BT4460at 5871.135 kb on - strand, within BT4460at 5871.148 kb on + strand, within BT4460at 5871.149 kb on - strand, within BT4460at 5871.224 kb on + strand, within BT4460at 5871.225 kb on - strand, within BT4460at 5871.341 kb on - strand, within BT4460at 5871.351 kb on + strand, within BT4460at 5871.456 kb on + strand, within BT4460at 5871.457 kb on - strand, within BT4460at 5871.471 kb on + strand, within BT4460at 5871.542 kb on - strand, within BT4460at 5871.542 kb on - strand, within BT4460at 5871.542 kb on - strand, within BT4460at 5871.543 kb on + strand, within BT4460at 5871.666 kb on + strand, within BT4460at 5871.670 kb on + strand, within BT4460at 5871.670 kb on + strand, within BT4460at 5871.694 kb on - strand, within BT4460at 5871.713 kb on + strandat 5871.734 kb on - strand, within BT4460at 5871.734 kb on - strand, within BT4460at 5871.770 kb on + strand, within BT4460at 5871.771 kb on - strand, within BT4460at 5871.775 kb on - strand, within BT4460at 5871.848 kb on - strandat 5871.944 kb on - strandat 5871.944 kb on - strandat 5871.963 kb on + strandat 5872.094 kb on + strand, within BT4461at 5872.183 kb on - strand, within BT4461at 5872.211 kb on + strand, within BT4461at 5872.215 kb on - strand, within BT4461at 5872.411 kb on + strand, within BT4461at 5872.426 kb on + strand, within BT4461at 5872.435 kb on + strand, within BT4461at 5872.436 kb on - strand, within BT4461at 5872.472 kb on + strand, within BT4461at 5872.473 kb on - strand, within BT4461at 5872.519 kb on - strandat 5872.566 kb on + strandat 5872.566 kb on + strandat 5872.735 kb on + strand, within BT4462at 5872.975 kb on - strand, within BT4462at 5873.162 kb on - strandat 5873.162 kb on - strandat 5873.164 kb on - strandat 5873.165 kb on + strandat 5873.168 kb on + strandat 5873.221 kb on + strandat 5873.231 kb on - strandat 5873.313 kb on - strand, within BT4463at 5873.313 kb on - strand, within BT4463at 5873.313 kb on - strand, within BT4463at 5873.313 kb on - strand, within BT4463at 5873.362 kb on - strand, within BT4463at 5873.449 kb on + strand, within BT4463at 5873.449 kb on + strand, within BT4463at 5873.455 kb on - strand, within BT4463at 5873.494 kb on + strand, within BT4463

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM
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5,871,010 + BT4460 0.29 -1.0
5,871,038 - BT4460 0.31 +1.9
5,871,040 + BT4460 0.32 +0.0
5,871,041 - BT4460 0.32 -4.0
5,871,079 + BT4460 0.34 +0.6
5,871,080 - BT4460 0.35 +0.2
5,871,080 - BT4460 0.35 -0.7
5,871,087 + BT4460 0.35 +1.6
5,871,087 + BT4460 0.35 -1.0
5,871,111 + BT4460 0.37 +0.1
5,871,111 + BT4460 0.37 -1.0
5,871,133 - BT4460 0.38 -0.4
5,871,135 - BT4460 0.39 +0.2
5,871,135 - BT4460 0.39 -0.3
5,871,148 + BT4460 0.40 +0.6
5,871,149 - BT4460 0.40 -0.6
5,871,224 + BT4460 0.45 +1.0
5,871,225 - BT4460 0.45 -1.0
5,871,341 - BT4460 0.54 -1.0
5,871,351 + BT4460 0.55 -0.6
5,871,456 + BT4460 0.62 +0.0
5,871,457 - BT4460 0.62 -1.0
5,871,471 + BT4460 0.63 +1.0
5,871,542 - BT4460 0.69 -3.5
5,871,542 - BT4460 0.69 -1.0
5,871,542 - BT4460 0.69 -1.2
5,871,543 + BT4460 0.69 +1.3
5,871,666 + BT4460 0.78 -1.6
5,871,670 + BT4460 0.78 +0.0
5,871,670 + BT4460 0.78 +0.0
5,871,694 - BT4460 0.80 -1.4
5,871,713 + +0.4
5,871,734 - BT4460 0.83 -1.1
5,871,734 - BT4460 0.83 +2.6
5,871,770 + BT4460 0.86 -3.2
5,871,771 - BT4460 0.86 +0.0
5,871,775 - BT4460 0.86 -0.3
5,871,848 - -1.6
5,871,944 - +2.3
5,871,944 - +3.0
5,871,963 + +1.0
5,872,094 + BT4461 0.23 +0.0
5,872,183 - BT4461 0.39 +0.3
5,872,211 + BT4461 0.44 +0.0
5,872,215 - BT4461 0.45 -1.6
5,872,411 + BT4461 0.79 -1.9
5,872,426 + BT4461 0.81 +0.0
5,872,435 + BT4461 0.83 +1.0
5,872,436 - BT4461 0.83 +4.0
5,872,472 + BT4461 0.89 +0.1
5,872,473 - BT4461 0.90 -2.6
5,872,519 - -1.6
5,872,566 + +2.9
5,872,566 + -3.0
5,872,735 + BT4462 0.30 -1.4
5,872,975 - BT4462 0.67 +1.6
5,873,162 - +1.0
5,873,162 - +2.2
5,873,164 - -0.4
5,873,165 + -1.1
5,873,168 + -1.3
5,873,221 + +0.8
5,873,231 - -0.5
5,873,313 - BT4463 0.10 +3.2
5,873,313 - BT4463 0.10 +1.9
5,873,313 - BT4463 0.10 -1.8
5,873,313 - BT4463 0.10 -1.0
5,873,362 - BT4463 0.15 +1.6
5,873,449 + BT4463 0.24 +2.3
5,873,449 + BT4463 0.24 -2.4
5,873,455 - BT4463 0.25 -0.6
5,873,494 + BT4463 0.29 +1.6

Or see this region's nucleotide sequence