Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4460 and BT4461 overlap by 4 nucleotides BT4461 and BT4462 are separated by 5 nucleotides BT4462 and BT4463 are separated by 25 nucleotides
BT4460: BT4460 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,870,614 to 5,871,963
BT4460
BT4461: BT4461 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,871,960 to 5,872,532
BT4461
BT4462: BT4462 - conserved hypothetical protein (NCBI ptt file), at 5,872,538 to 5,873,191
BT4462
BT4463: BT4463 - phosphatidate cytidylyltransferase (NCBI ptt file), at 5,873,217 to 5,874,173
BT4463
Position (kb)
5871
5872
5873 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5871.010 kb on + strand, within BT4460 at 5871.038 kb on - strand, within BT4460 at 5871.040 kb on + strand, within BT4460 at 5871.041 kb on - strand, within BT4460 at 5871.079 kb on + strand, within BT4460 at 5871.080 kb on - strand, within BT4460 at 5871.080 kb on - strand, within BT4460 at 5871.087 kb on + strand, within BT4460 at 5871.087 kb on + strand, within BT4460 at 5871.111 kb on + strand, within BT4460 at 5871.111 kb on + strand, within BT4460 at 5871.133 kb on - strand, within BT4460 at 5871.135 kb on - strand, within BT4460 at 5871.135 kb on - strand, within BT4460 at 5871.148 kb on + strand, within BT4460 at 5871.149 kb on - strand, within BT4460 at 5871.224 kb on + strand, within BT4460 at 5871.225 kb on - strand, within BT4460 at 5871.341 kb on - strand, within BT4460 at 5871.351 kb on + strand, within BT4460 at 5871.456 kb on + strand, within BT4460 at 5871.457 kb on - strand, within BT4460 at 5871.471 kb on + strand, within BT4460 at 5871.542 kb on - strand, within BT4460 at 5871.542 kb on - strand, within BT4460 at 5871.542 kb on - strand, within BT4460 at 5871.543 kb on + strand, within BT4460 at 5871.666 kb on + strand, within BT4460 at 5871.670 kb on + strand, within BT4460 at 5871.670 kb on + strand, within BT4460 at 5871.694 kb on - strand, within BT4460 at 5871.713 kb on + strand at 5871.734 kb on - strand, within BT4460 at 5871.734 kb on - strand, within BT4460 at 5871.770 kb on + strand, within BT4460 at 5871.771 kb on - strand, within BT4460 at 5871.775 kb on - strand, within BT4460 at 5871.848 kb on - strand at 5871.944 kb on - strand at 5871.944 kb on - strand at 5871.963 kb on + strand at 5872.094 kb on + strand, within BT4461 at 5872.183 kb on - strand, within BT4461 at 5872.211 kb on + strand, within BT4461 at 5872.215 kb on - strand, within BT4461 at 5872.411 kb on + strand, within BT4461 at 5872.426 kb on + strand, within BT4461 at 5872.435 kb on + strand, within BT4461 at 5872.436 kb on - strand, within BT4461 at 5872.472 kb on + strand, within BT4461 at 5872.473 kb on - strand, within BT4461 at 5872.519 kb on - strand at 5872.566 kb on + strand at 5872.566 kb on + strand at 5872.735 kb on + strand, within BT4462 at 5872.975 kb on - strand, within BT4462 at 5873.162 kb on - strand at 5873.162 kb on - strand at 5873.164 kb on - strand at 5873.165 kb on + strand at 5873.168 kb on + strand at 5873.221 kb on + strand at 5873.231 kb on - strand at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.313 kb on - strand, within BT4463 at 5873.362 kb on - strand, within BT4463 at 5873.449 kb on + strand, within BT4463 at 5873.449 kb on + strand, within BT4463 at 5873.455 kb on - strand, within BT4463 at 5873.494 kb on + strand, within BT4463
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 5,871,010 + BT4460 0.29 -1.0 5,871,038 - BT4460 0.31 +1.9 5,871,040 + BT4460 0.32 +0.0 5,871,041 - BT4460 0.32 -4.0 5,871,079 + BT4460 0.34 +0.6 5,871,080 - BT4460 0.35 +0.2 5,871,080 - BT4460 0.35 -0.7 5,871,087 + BT4460 0.35 +1.6 5,871,087 + BT4460 0.35 -1.0 5,871,111 + BT4460 0.37 +0.1 5,871,111 + BT4460 0.37 -1.0 5,871,133 - BT4460 0.38 -0.4 5,871,135 - BT4460 0.39 +0.2 5,871,135 - BT4460 0.39 -0.3 5,871,148 + BT4460 0.40 +0.6 5,871,149 - BT4460 0.40 -0.6 5,871,224 + BT4460 0.45 +1.0 5,871,225 - BT4460 0.45 -1.0 5,871,341 - BT4460 0.54 -1.0 5,871,351 + BT4460 0.55 -0.6 5,871,456 + BT4460 0.62 +0.0 5,871,457 - BT4460 0.62 -1.0 5,871,471 + BT4460 0.63 +1.0 5,871,542 - BT4460 0.69 -3.5 5,871,542 - BT4460 0.69 -1.0 5,871,542 - BT4460 0.69 -1.2 5,871,543 + BT4460 0.69 +1.3 5,871,666 + BT4460 0.78 -1.6 5,871,670 + BT4460 0.78 +0.0 5,871,670 + BT4460 0.78 +0.0 5,871,694 - BT4460 0.80 -1.4 5,871,713 + +0.4 5,871,734 - BT4460 0.83 -1.1 5,871,734 - BT4460 0.83 +2.6 5,871,770 + BT4460 0.86 -3.2 5,871,771 - BT4460 0.86 +0.0 5,871,775 - BT4460 0.86 -0.3 5,871,848 - -1.6 5,871,944 - +2.3 5,871,944 - +3.0 5,871,963 + +1.0 5,872,094 + BT4461 0.23 +0.0 5,872,183 - BT4461 0.39 +0.3 5,872,211 + BT4461 0.44 +0.0 5,872,215 - BT4461 0.45 -1.6 5,872,411 + BT4461 0.79 -1.9 5,872,426 + BT4461 0.81 +0.0 5,872,435 + BT4461 0.83 +1.0 5,872,436 - BT4461 0.83 +4.0 5,872,472 + BT4461 0.89 +0.1 5,872,473 - BT4461 0.90 -2.6 5,872,519 - -1.6 5,872,566 + +2.9 5,872,566 + -3.0 5,872,735 + BT4462 0.30 -1.4 5,872,975 - BT4462 0.67 +1.6 5,873,162 - +1.0 5,873,162 - +2.2 5,873,164 - -0.4 5,873,165 + -1.1 5,873,168 + -1.3 5,873,221 + +0.8 5,873,231 - -0.5 5,873,313 - BT4463 0.10 +3.2 5,873,313 - BT4463 0.10 +1.9 5,873,313 - BT4463 0.10 -1.8 5,873,313 - BT4463 0.10 -1.0 5,873,362 - BT4463 0.15 +1.6 5,873,449 + BT4463 0.24 +2.3 5,873,449 + BT4463 0.24 -2.4 5,873,455 - BT4463 0.25 -0.6 5,873,494 + BT4463 0.29 +1.6
Or see this region's nucleotide sequence