Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3821 and BT3822 are separated by 156 nucleotides BT3822 and BT3823 are separated by 252 nucleotides BT3823 and BT3824 are separated by 25 nucleotides
BT3821: BT3821 - 5,10-methylenetetrahydrofolate reductase (NCBI ptt file), at 4,973,330 to 4,974,283
BT3821
BT3822: BT3822 - hypothetical protein (NCBI ptt file), at 4,974,440 to 4,974,970
BT3822
BT3823: BT3823 - conserved hypothetical protein (NCBI ptt file), at 4,975,223 to 4,975,735
BT3823
BT3824: BT3824 - hypothetical protein (NCBI ptt file), at 4,975,761 to 4,976,045
BT3824
Position (kb)
4974
4975 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4973.589 kb on - strand, within BT3821 at 4973.589 kb on - strand, within BT3821 at 4973.589 kb on - strand, within BT3821 at 4973.589 kb on - strand, within BT3821 at 4973.589 kb on - strand, within BT3821 at 4973.597 kb on - strand, within BT3821 at 4973.652 kb on - strand, within BT3821 at 4973.805 kb on - strand, within BT3821 at 4973.906 kb on - strand, within BT3821 at 4973.961 kb on - strand, within BT3821 at 4973.965 kb on - strand, within BT3821 at 4974.000 kb on - strand, within BT3821 at 4974.018 kb on - strand, within BT3821 at 4974.025 kb on - strand, within BT3821 at 4974.113 kb on - strand, within BT3821 at 4974.117 kb on - strand, within BT3821 at 4974.121 kb on - strand, within BT3821 at 4974.149 kb on - strand, within BT3821 at 4974.151 kb on - strand, within BT3821 at 4974.190 kb on - strand at 4974.260 kb on - strand at 4974.264 kb on - strand at 4974.342 kb on - strand at 4974.400 kb on + strand at 4974.400 kb on + strand at 4974.400 kb on + strand at 4974.401 kb on - strand at 4974.403 kb on + strand at 4974.403 kb on + strand at 4974.403 kb on + strand at 4974.407 kb on + strand at 4974.445 kb on + strand at 4974.445 kb on + strand at 4974.525 kb on + strand, within BT3822 at 4974.526 kb on - strand, within BT3822 at 4974.625 kb on + strand, within BT3822 at 4974.626 kb on - strand, within BT3822 at 4974.626 kb on - strand, within BT3822 at 4974.694 kb on - strand, within BT3822 at 4974.694 kb on - strand, within BT3822 at 4974.729 kb on + strand, within BT3822 at 4974.729 kb on + strand, within BT3822 at 4974.762 kb on + strand, within BT3822 at 4974.762 kb on + strand, within BT3822 at 4974.763 kb on - strand, within BT3822 at 4974.782 kb on + strand, within BT3822 at 4974.782 kb on + strand, within BT3822 at 4974.782 kb on + strand, within BT3822 at 4974.783 kb on - strand, within BT3822 at 4974.783 kb on - strand, within BT3822 at 4974.801 kb on + strand, within BT3822 at 4974.801 kb on + strand, within BT3822 at 4974.803 kb on + strand, within BT3822 at 4974.806 kb on + strand, within BT3822 at 4974.806 kb on + strand, within BT3822 at 4974.806 kb on + strand, within BT3822 at 4974.854 kb on + strand, within BT3822 at 4974.873 kb on - strand, within BT3822 at 4974.875 kb on + strand, within BT3822 at 4974.993 kb on + strand at 4974.996 kb on - strand at 4974.999 kb on + strand at 4974.999 kb on + strand at 4975.000 kb on - strand at 4975.007 kb on - strand at 4975.015 kb on - strand at 4975.133 kb on + strand at 4975.133 kb on + strand at 4975.134 kb on - strand at 4975.225 kb on - strand at 4975.312 kb on + strand, within BT3823 at 4975.313 kb on - strand, within BT3823 at 4975.334 kb on - strand, within BT3823 at 4975.355 kb on - strand, within BT3823 at 4975.408 kb on + strand, within BT3823 at 4975.409 kb on - strand, within BT3823 at 4975.494 kb on - strand, within BT3823 at 4975.583 kb on + strand, within BT3823 at 4975.584 kb on - strand, within BT3823 at 4975.584 kb on - strand, within BT3823 at 4975.584 kb on - strand, within BT3823 at 4975.608 kb on - strand, within BT3823 at 4975.610 kb on + strand, within BT3823 at 4975.613 kb on + strand, within BT3823 at 4975.614 kb on - strand, within BT3823 at 4975.636 kb on - strand, within BT3823 at 4975.639 kb on - strand, within BT3823 at 4975.639 kb on - strand, within BT3823 at 4975.691 kb on + strand at 4975.691 kb on + strand at 4975.748 kb on + strand at 4975.759 kb on - strand at 4975.817 kb on + strand, within BT3824 at 4975.829 kb on + strand, within BT3824 at 4975.831 kb on + strand, within BT3824 at 4975.832 kb on - strand, within BT3824 at 4975.833 kb on + strand, within BT3824 at 4975.833 kb on + strand, within BT3824 at 4975.833 kb on + strand, within BT3824 at 4975.842 kb on + strand, within BT3824 at 4975.842 kb on + strand, within BT3824 at 4975.873 kb on - strand, within BT3824 at 4975.875 kb on + strand, within BT3824 at 4975.876 kb on - strand, within BT3824 at 4975.885 kb on + strand, within BT3824 at 4975.886 kb on - strand, within BT3824 at 4975.929 kb on + strand, within BT3824 at 4975.958 kb on - strand, within BT3824
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 4,973,589 - BT3821 0.27 +1.1 4,973,589 - BT3821 0.27 -2.1 4,973,589 - BT3821 0.27 -0.9 4,973,589 - BT3821 0.27 -1.7 4,973,589 - BT3821 0.27 -0.2 4,973,597 - BT3821 0.28 -1.5 4,973,652 - BT3821 0.34 +0.2 4,973,805 - BT3821 0.50 +0.7 4,973,906 - BT3821 0.60 -1.9 4,973,961 - BT3821 0.66 +3.5 4,973,965 - BT3821 0.67 -1.4 4,974,000 - BT3821 0.70 -0.8 4,974,018 - BT3821 0.72 -0.4 4,974,025 - BT3821 0.73 -0.9 4,974,113 - BT3821 0.82 -1.2 4,974,117 - BT3821 0.82 +0.1 4,974,121 - BT3821 0.83 -1.3 4,974,149 - BT3821 0.86 -0.3 4,974,151 - BT3821 0.86 +1.7 4,974,190 - -1.5 4,974,260 - -0.3 4,974,264 - -1.7 4,974,342 - +0.1 4,974,400 + -1.4 4,974,400 + +1.1 4,974,400 + +1.7 4,974,401 - -2.3 4,974,403 + +0.1 4,974,403 + +0.9 4,974,403 + -1.5 4,974,407 + +1.1 4,974,445 + -2.5 4,974,445 + -1.5 4,974,525 + BT3822 0.16 +2.1 4,974,526 - BT3822 0.16 -2.6 4,974,625 + BT3822 0.35 -2.3 4,974,626 - BT3822 0.35 +0.7 4,974,626 - BT3822 0.35 -0.4 4,974,694 - BT3822 0.48 -2.5 4,974,694 - BT3822 0.48 -0.5 4,974,729 + BT3822 0.54 -2.7 4,974,729 + BT3822 0.54 -4.0 4,974,762 + BT3822 0.61 +0.1 4,974,762 + BT3822 0.61 -1.5 4,974,763 - BT3822 0.61 -1.2 4,974,782 + BT3822 0.64 -2.5 4,974,782 + BT3822 0.64 -3.7 4,974,782 + BT3822 0.64 -2.5 4,974,783 - BT3822 0.65 -0.5 4,974,783 - BT3822 0.65 +2.2 4,974,801 + BT3822 0.68 -0.8 4,974,801 + BT3822 0.68 -2.2 4,974,803 + BT3822 0.68 -1.4 4,974,806 + BT3822 0.69 -2.5 4,974,806 + BT3822 0.69 -0.8 4,974,806 + BT3822 0.69 +0.6 4,974,854 + BT3822 0.78 -2.1 4,974,873 - BT3822 0.82 +0.7 4,974,875 + BT3822 0.82 -3.1 4,974,993 + -2.3 4,974,996 - -1.5 4,974,999 + -0.0 4,974,999 + -1.9 4,975,000 - -1.0 4,975,007 - -0.1 4,975,015 - +0.2 4,975,133 + -0.3 4,975,133 + +0.5 4,975,134 - +0.7 4,975,225 - -1.4 4,975,312 + BT3823 0.17 +0.7 4,975,313 - BT3823 0.18 +1.7 4,975,334 - BT3823 0.22 -2.9 4,975,355 - BT3823 0.26 +1.1 4,975,408 + BT3823 0.36 -0.7 4,975,409 - BT3823 0.36 -4.1 4,975,494 - BT3823 0.53 -2.5 4,975,583 + BT3823 0.70 -0.9 4,975,584 - BT3823 0.70 -3.3 4,975,584 - BT3823 0.70 +0.1 4,975,584 - BT3823 0.70 -1.5 4,975,608 - BT3823 0.75 -2.5 4,975,610 + BT3823 0.75 -0.9 4,975,613 + BT3823 0.76 -1.3 4,975,614 - BT3823 0.76 -1.7 4,975,636 - BT3823 0.81 -0.7 4,975,639 - BT3823 0.81 -0.3 4,975,639 - BT3823 0.81 -1.9 4,975,691 + -1.0 4,975,691 + -0.9 4,975,748 + +0.1 4,975,759 - +1.1 4,975,817 + BT3824 0.20 +1.1 4,975,829 + BT3824 0.24 -1.5 4,975,831 + BT3824 0.25 +1.4 4,975,832 - BT3824 0.25 -1.7 4,975,833 + BT3824 0.25 +2.1 4,975,833 + BT3824 0.25 -0.6 4,975,833 + BT3824 0.25 -1.1 4,975,842 + BT3824 0.28 +0.7 4,975,842 + BT3824 0.28 +3.2 4,975,873 - BT3824 0.39 +2.1 4,975,875 + BT3824 0.40 +2.6 4,975,876 - BT3824 0.40 -2.7 4,975,885 + BT3824 0.44 +0.5 4,975,886 - BT3824 0.44 -1.4 4,975,929 + BT3824 0.59 +0.1 4,975,958 - BT3824 0.69 +1.4
Or see this region's nucleotide sequence