Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3249 and BT3250 are separated by 2 nucleotides BT3250 and BT3251 are separated by 46 nucleotides BT3251 and BT3252 overlap by 16 nucleotides BT3252 and BT3253 overlap by 1 nucleotides
BT3249: BT3249 - putative zinc ABC transporter, zinc-binding protein (NCBI ptt file), at 4,148,994 to 4,149,920
BT3249
BT3250: BT3250 - conserved hypothetical protein (NCBI ptt file), at 4,149,923 to 4,150,645
BT3250
BT3251: BT3251 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 4,150,692 to 4,151,171
BT3251
BT3252: BT3252 - hypothetical protein (NCBI ptt file), at 4,151,156 to 4,152,166
BT3252
BT3253: BT3253 - phosphoribosylamine--glycine ligase (NCBI ptt file), at 4,152,166 to 4,153,440
BT3253
Position (kb)
4150
4151
4152 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4149.700 kb on + strand, within BT3249 at 4149.700 kb on + strand, within BT3249 at 4149.701 kb on - strand, within BT3249 at 4149.701 kb on - strand, within BT3249 at 4149.924 kb on + strand at 4149.929 kb on + strand at 4149.930 kb on - strand at 4149.930 kb on - strand at 4149.971 kb on + strand at 4149.972 kb on - strand at 4149.972 kb on - strand at 4150.066 kb on - strand, within BT3250 at 4150.066 kb on - strand, within BT3250 at 4150.066 kb on - strand, within BT3250 at 4150.066 kb on - strand at 4150.095 kb on - strand, within BT3250 at 4150.102 kb on - strand, within BT3250 at 4150.168 kb on - strand, within BT3250 at 4150.254 kb on - strand, within BT3250 at 4150.280 kb on - strand, within BT3250 at 4150.286 kb on + strand, within BT3250 at 4150.309 kb on + strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.351 kb on + strand, within BT3250 at 4150.353 kb on + strand, within BT3250 at 4150.406 kb on - strand, within BT3250 at 4150.406 kb on - strand, within BT3250 at 4150.408 kb on - strand, within BT3250 at 4150.412 kb on + strand, within BT3250 at 4150.413 kb on - strand, within BT3250 at 4150.413 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.448 kb on - strand, within BT3250 at 4150.537 kb on + strand, within BT3250 at 4150.538 kb on - strand, within BT3250 at 4150.665 kb on - strand at 4150.665 kb on - strand at 4150.667 kb on + strand at 4150.675 kb on - strand at 4150.679 kb on - strand at 4150.689 kb on - strand at 4150.725 kb on + strand at 4150.725 kb on + strand at 4150.730 kb on - strand at 4150.730 kb on - strand at 4150.744 kb on + strand, within BT3251 at 4150.744 kb on + strand, within BT3251 at 4150.745 kb on - strand, within BT3251 at 4150.745 kb on - strand, within BT3251 at 4150.750 kb on - strand, within BT3251 at 4150.791 kb on - strand, within BT3251 at 4150.791 kb on - strand, within BT3251 at 4150.794 kb on - strand, within BT3251 at 4150.966 kb on + strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.970 kb on - strand, within BT3251 at 4150.970 kb on - strand, within BT3251 at 4151.016 kb on - strand, within BT3251 at 4151.053 kb on - strand at 4151.084 kb on + strand, within BT3251 at 4151.085 kb on - strand, within BT3251 at 4151.098 kb on - strand, within BT3251 at 4151.146 kb on - strand at 4152.170 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 4,149,700 + BT3249 0.76 +0.2 4,149,700 + BT3249 0.76 -2.5 4,149,701 - BT3249 0.76 +0.2 4,149,701 - BT3249 0.76 -0.8 4,149,924 + -2.0 4,149,929 + -1.0 4,149,930 - +2.2 4,149,930 - -1.6 4,149,971 + -1.5 4,149,972 - +2.7 4,149,972 - +2.7 4,150,066 - BT3250 0.20 +4.7 4,150,066 - BT3250 0.20 +1.2 4,150,066 - BT3250 0.20 +1.4 4,150,066 - +0.2 4,150,095 - BT3250 0.24 +0.2 4,150,102 - BT3250 0.25 +2.7 4,150,168 - BT3250 0.34 +2.3 4,150,254 - BT3250 0.46 +0.5 4,150,280 - BT3250 0.49 +3.3 4,150,286 + BT3250 0.50 -1.6 4,150,309 + BT3250 0.53 -1.8 4,150,350 - BT3250 0.59 -0.1 4,150,350 - BT3250 0.59 +2.8 4,150,350 - BT3250 0.59 -1.9 4,150,350 - BT3250 0.59 +1.2 4,150,351 + BT3250 0.59 -2.8 4,150,353 + BT3250 0.59 -3.0 4,150,406 - BT3250 0.67 +1.1 4,150,406 - BT3250 0.67 +1.2 4,150,408 - BT3250 0.67 +2.7 4,150,412 + BT3250 0.68 -0.5 4,150,413 - BT3250 0.68 +0.6 4,150,413 - BT3250 0.68 -0.5 4,150,418 - BT3250 0.68 -1.0 4,150,418 - BT3250 0.68 -0.6 4,150,418 - BT3250 0.68 +2.5 4,150,418 - BT3250 0.68 +0.2 4,150,448 - BT3250 0.73 -0.5 4,150,537 + BT3250 0.85 -2.1 4,150,538 - BT3250 0.85 -0.9 4,150,665 - +3.8 4,150,665 - +2.5 4,150,667 + +0.2 4,150,675 - +1.5 4,150,679 - +3.0 4,150,689 - +1.6 4,150,725 + -0.9 4,150,725 + -1.9 4,150,730 - +2.1 4,150,730 - +2.4 4,150,744 + BT3251 0.11 -1.4 4,150,744 + BT3251 0.11 -2.4 4,150,745 - BT3251 0.11 +2.4 4,150,745 - BT3251 0.11 +3.2 4,150,750 - BT3251 0.12 +3.7 4,150,791 - BT3251 0.21 +1.4 4,150,791 - BT3251 0.21 +2.5 4,150,794 - BT3251 0.21 +2.9 4,150,966 + BT3251 0.57 -1.8 4,150,967 - BT3251 0.57 +4.7 4,150,967 - BT3251 0.57 +3.3 4,150,967 - BT3251 0.57 +1.0 4,150,967 - BT3251 0.57 +4.5 4,150,967 - BT3251 0.57 +2.2 4,150,970 - BT3251 0.58 +1.3 4,150,970 - BT3251 0.58 +0.7 4,151,016 - BT3251 0.68 +2.7 4,151,053 - +1.5 4,151,084 + BT3251 0.82 -0.8 4,151,085 - BT3251 0.82 +1.5 4,151,098 - BT3251 0.85 +1.1 4,151,146 - +3.3 4,152,170 - -3.3
Or see this region's nucleotide sequence