Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3040 and BT3041 are separated by 18 nucleotides BT3041 and BT3042 overlap by 80 nucleotides BT3042 and BT3043 are separated by 143 nucleotides
BT3040: BT3040 - conserved hypothetical protein (NCBI ptt file), at 3,849,918 to 3,850,934
BT3040
BT3041: BT3041 - hypothetical protein (NCBI ptt file), at 3,850,953 to 3,851,591
BT3041
BT3042: BT3042 - conserved hypothetical protein (NCBI ptt file), at 3,851,512 to 3,851,889
BT3042
BT3043: BT3043 - putative xylanase (NCBI ptt file), at 3,852,033 to 3,853,619
BT3043
Position (kb)
3851
3852 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3850.691 kb on - strand, within BT3040 at 3850.698 kb on - strand, within BT3040 at 3850.718 kb on + strand, within BT3040 at 3850.718 kb on + strand, within BT3040 at 3850.719 kb on - strand, within BT3040 at 3850.719 kb on - strand, within BT3040 at 3850.729 kb on - strand, within BT3040 at 3850.731 kb on - strand, within BT3040 at 3850.732 kb on + strand, within BT3040 at 3850.732 kb on + strand, within BT3040 at 3850.732 kb on + strand, within BT3040 at 3850.732 kb on + strand, within BT3040 at 3850.733 kb on - strand, within BT3040 at 3850.733 kb on - strand, within BT3040 at 3850.842 kb on + strand at 3850.937 kb on + strand at 3850.938 kb on - strand at 3851.046 kb on - strand, within BT3041 at 3851.059 kb on - strand, within BT3041 at 3851.107 kb on + strand, within BT3041 at 3851.108 kb on - strand, within BT3041 at 3851.345 kb on - strand, within BT3041 at 3851.374 kb on + strand, within BT3041 at 3851.375 kb on - strand, within BT3041 at 3851.405 kb on + strand, within BT3041 at 3851.406 kb on - strand, within BT3041 at 3851.456 kb on + strand, within BT3041 at 3851.474 kb on + strand, within BT3041 at 3851.475 kb on - strand, within BT3041 at 3851.510 kb on + strand, within BT3041 at 3851.517 kb on - strand at 3851.556 kb on + strand at 3851.557 kb on - strand at 3851.557 kb on - strand at 3851.584 kb on + strand at 3851.619 kb on + strand, within BT3042 at 3851.727 kb on + strand, within BT3042 at 3851.736 kb on + strand, within BT3042 at 3851.737 kb on - strand, within BT3042 at 3851.872 kb on - strand at 3851.874 kb on + strand at 3851.874 kb on + strand at 3851.874 kb on + strand at 3851.874 kb on + strand at 3851.875 kb on - strand at 3851.906 kb on + strand at 3851.935 kb on + strand at 3852.029 kb on + strand at 3852.030 kb on - strand at 3852.030 kb on - strand at 3852.031 kb on + strand at 3852.051 kb on + strand at 3852.052 kb on - strand at 3852.058 kb on - strand at 3852.060 kb on - strand at 3852.133 kb on - strand at 3852.133 kb on - strand at 3852.178 kb on - strand at 3852.183 kb on - strand at 3852.214 kb on + strand, within BT3043 at 3852.215 kb on - strand, within BT3043 at 3852.236 kb on - strand, within BT3043 at 3852.370 kb on + strand, within BT3043 at 3852.370 kb on + strand, within BT3043 at 3852.371 kb on - strand, within BT3043 at 3852.371 kb on - strand, within BT3043 at 3852.461 kb on - strand, within BT3043 at 3852.510 kb on + strand, within BT3043 at 3852.510 kb on + strand, within BT3043 at 3852.530 kb on + strand, within BT3043 at 3852.541 kb on + strand, within BT3043 at 3852.541 kb on + strand, within BT3043 at 3852.608 kb on + strand, within BT3043 at 3852.640 kb on + strand, within BT3043 at 3852.641 kb on - strand, within BT3043 at 3852.641 kb on - strand, within BT3043 at 3852.641 kb on - strand, within BT3043 at 3852.641 kb on - strand, within BT3043 at 3852.758 kb on - strand, within BT3043 at 3852.762 kb on - strand, within BT3043 at 3852.836 kb on + strand, within BT3043 at 3852.867 kb on - strand, within BT3043
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 3,850,691 - BT3040 0.76 +1.2 3,850,698 - BT3040 0.77 +0.5 3,850,718 + BT3040 0.79 -1.4 3,850,718 + BT3040 0.79 -0.4 3,850,719 - BT3040 0.79 -0.8 3,850,719 - BT3040 0.79 -0.4 3,850,729 - BT3040 0.80 +2.8 3,850,731 - BT3040 0.80 -2.4 3,850,732 + BT3040 0.80 +3.0 3,850,732 + BT3040 0.80 -2.2 3,850,732 + BT3040 0.80 -0.4 3,850,732 + BT3040 0.80 -0.8 3,850,733 - BT3040 0.80 -2.0 3,850,733 - BT3040 0.80 -0.6 3,850,842 + -0.5 3,850,937 + -1.5 3,850,938 - -4.3 3,851,046 - BT3041 0.15 +0.6 3,851,059 - BT3041 0.17 +1.2 3,851,107 + BT3041 0.24 -1.1 3,851,108 - BT3041 0.24 +2.3 3,851,345 - BT3041 0.61 -0.5 3,851,374 + BT3041 0.66 -1.2 3,851,375 - BT3041 0.66 +1.6 3,851,405 + BT3041 0.71 +2.7 3,851,406 - BT3041 0.71 +1.9 3,851,456 + BT3041 0.79 +0.2 3,851,474 + BT3041 0.82 +1.2 3,851,475 - BT3041 0.82 -0.8 3,851,510 + BT3041 0.87 +1.3 3,851,517 - +0.6 3,851,556 + +2.1 3,851,557 - +0.9 3,851,557 - -1.6 3,851,584 + -0.5 3,851,619 + BT3042 0.28 +1.2 3,851,727 + BT3042 0.57 +1.8 3,851,736 + BT3042 0.59 +3.2 3,851,737 - BT3042 0.60 -0.6 3,851,872 - +0.9 3,851,874 + -0.8 3,851,874 + -1.4 3,851,874 + -2.1 3,851,874 + -1.2 3,851,875 - -0.5 3,851,906 + +1.9 3,851,935 + +1.2 3,852,029 + -0.5 3,852,030 - -0.6 3,852,030 - +2.2 3,852,031 + +0.4 3,852,051 + -1.2 3,852,052 - -1.7 3,852,058 - -1.4 3,852,060 - -1.4 3,852,133 - +3.2 3,852,133 - -1.7 3,852,178 - -2.1 3,852,183 - -0.4 3,852,214 + BT3043 0.11 +0.4 3,852,215 - BT3043 0.11 +1.1 3,852,236 - BT3043 0.13 -2.1 3,852,370 + BT3043 0.21 -0.7 3,852,370 + BT3043 0.21 +1.8 3,852,371 - BT3043 0.21 +2.2 3,852,371 - BT3043 0.21 -0.8 3,852,461 - BT3043 0.27 +0.9 3,852,510 + BT3043 0.30 -1.1 3,852,510 + BT3043 0.30 -1.8 3,852,530 + BT3043 0.31 -2.6 3,852,541 + BT3043 0.32 -2.5 3,852,541 + BT3043 0.32 +0.2 3,852,608 + BT3043 0.36 -2.5 3,852,640 + BT3043 0.38 +3.6 3,852,641 - BT3043 0.38 +0.2 3,852,641 - BT3043 0.38 -1.5 3,852,641 - BT3043 0.38 -1.4 3,852,641 - BT3043 0.38 +0.2 3,852,758 - BT3043 0.46 -0.7 3,852,762 - BT3043 0.46 +0.7 3,852,836 + BT3043 0.51 -0.8 3,852,867 - BT3043 0.53 +0.0
Or see this region's nucleotide sequence