Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2999 and BT3000 are separated by 173 nucleotides BT3000 and BT3001 are separated by 116 nucleotides
BT2999: BT2999 - helicase IV (NCBI ptt file), at 3,778,947 to 3,781,559
BT2999
BT3000: BT3000 - conserved hypothetical protein (NCBI ptt file), at 3,781,733 to 3,782,362
BT3000
BT3001: BT3001 - ATP-dependent DNA helicase (NCBI ptt file), at 3,782,479 to 3,784,062
BT3001
Position (kb)
3781
3782
3783 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3780.733 kb on + strand, within BT2999 at 3780.733 kb on + strand, within BT2999 at 3780.735 kb on + strand, within BT2999 at 3780.735 kb on + strand, within BT2999 at 3780.736 kb on - strand, within BT2999 at 3780.756 kb on - strand, within BT2999 at 3780.802 kb on + strand, within BT2999 at 3780.807 kb on - strand, within BT2999 at 3780.811 kb on + strand, within BT2999 at 3780.811 kb on + strand, within BT2999 at 3780.872 kb on + strand, within BT2999 at 3780.873 kb on - strand, within BT2999 at 3780.873 kb on - strand, within BT2999 at 3780.961 kb on - strand, within BT2999 at 3780.973 kb on + strand, within BT2999 at 3780.984 kb on - strand, within BT2999 at 3780.993 kb on - strand, within BT2999 at 3781.004 kb on + strand, within BT2999 at 3781.004 kb on + strand, within BT2999 at 3781.029 kb on + strand, within BT2999 at 3781.030 kb on - strand, within BT2999 at 3781.048 kb on - strand, within BT2999 at 3781.063 kb on - strand, within BT2999 at 3781.082 kb on + strand, within BT2999 at 3781.092 kb on + strand, within BT2999 at 3781.096 kb on - strand, within BT2999 at 3781.099 kb on + strand, within BT2999 at 3781.102 kb on + strand, within BT2999 at 3781.103 kb on - strand, within BT2999 at 3781.103 kb on - strand, within BT2999 at 3781.161 kb on + strand, within BT2999 at 3781.161 kb on + strand, within BT2999 at 3781.175 kb on - strand, within BT2999 at 3781.185 kb on - strand, within BT2999 at 3781.191 kb on - strand, within BT2999 at 3781.194 kb on + strand, within BT2999 at 3781.214 kb on - strand, within BT2999 at 3781.214 kb on - strand, within BT2999 at 3781.232 kb on + strand, within BT2999 at 3781.236 kb on + strand, within BT2999 at 3781.237 kb on - strand, within BT2999 at 3781.237 kb on - strand, within BT2999 at 3781.248 kb on - strand at 3781.291 kb on - strand, within BT2999 at 3781.330 kb on + strand at 3781.355 kb on - strand at 3781.360 kb on + strand at 3781.360 kb on + strand at 3781.402 kb on - strand at 3781.402 kb on - strand at 3781.469 kb on + strand at 3781.478 kb on + strand at 3781.486 kb on + strand at 3781.491 kb on + strand at 3781.550 kb on + strand at 3781.567 kb on - strand at 3781.599 kb on + strand at 3781.600 kb on - strand at 3781.600 kb on - strand at 3781.617 kb on + strand at 3781.620 kb on + strand at 3781.620 kb on + strand at 3781.621 kb on - strand at 3781.626 kb on - strand at 3781.626 kb on - strand at 3781.626 kb on - strand at 3781.685 kb on - strand at 3781.711 kb on + strand at 3781.711 kb on + strand at 3781.711 kb on + strand at 3781.722 kb on + strand at 3781.722 kb on + strand at 3781.722 kb on + strand at 3781.722 kb on + strand at 3781.722 kb on + strand at 3781.723 kb on - strand at 3781.723 kb on - strand at 3781.724 kb on + strand at 3781.724 kb on + strand at 3781.724 kb on + strand at 3781.724 kb on + strand at 3781.725 kb on - strand at 3781.725 kb on - strand at 3781.726 kb on + strand at 3781.811 kb on + strand, within BT3000 at 3781.812 kb on - strand, within BT3000 at 3781.812 kb on - strand, within BT3000 at 3781.813 kb on + strand, within BT3000 at 3781.826 kb on - strand, within BT3000 at 3781.835 kb on + strand, within BT3000 at 3781.879 kb on - strand, within BT3000 at 3781.888 kb on + strand, within BT3000 at 3781.889 kb on - strand, within BT3000 at 3782.224 kb on + strand, within BT3000 at 3782.254 kb on + strand, within BT3000 at 3782.254 kb on + strand, within BT3000 at 3782.256 kb on + strand, within BT3000 at 3782.257 kb on - strand, within BT3000 at 3782.257 kb on - strand, within BT3000 at 3782.258 kb on + strand, within BT3000 at 3782.259 kb on - strand, within BT3000 at 3782.309 kb on + strand at 3782.343 kb on + strand at 3782.383 kb on + strand at 3782.384 kb on - strand at 3782.388 kb on + strand at 3782.468 kb on + strand at 3782.469 kb on - strand at 3782.469 kb on - strand at 3782.470 kb on + strand at 3782.560 kb on + strand at 3782.574 kb on - strand at 3782.574 kb on - strand at 3782.574 kb on - strand at 3782.574 kb on - strand at 3782.574 kb on - strand at 3782.592 kb on + strand at 3782.592 kb on + strand at 3782.592 kb on + strand at 3782.593 kb on - strand at 3782.593 kb on - strand at 3782.597 kb on + strand at 3782.608 kb on + strand at 3782.634 kb on + strand at 3782.635 kb on - strand at 3782.657 kb on - strand, within BT3001 at 3782.659 kb on + strand, within BT3001 at 3782.661 kb on + strand, within BT3001 at 3782.662 kb on - strand, within BT3001 at 3782.685 kb on + strand, within BT3001 at 3782.692 kb on + strand, within BT3001 at 3782.692 kb on + strand, within BT3001 at 3782.693 kb on - strand, within BT3001 at 3782.693 kb on - strand, within BT3001 at 3782.735 kb on - strand, within BT3001 at 3782.754 kb on + strand, within BT3001 at 3782.755 kb on - strand, within BT3001 at 3782.801 kb on - strand, within BT3001 at 3782.833 kb on - strand, within BT3001 at 3782.837 kb on - strand, within BT3001 at 3782.837 kb on - strand, within BT3001 at 3782.837 kb on - strand, within BT3001 at 3782.844 kb on + strand, within BT3001 at 3782.845 kb on - strand, within BT3001 at 3782.851 kb on - strand at 3782.853 kb on + strand, within BT3001 at 3782.881 kb on - strand, within BT3001 at 3782.926 kb on - strand, within BT3001 at 3782.945 kb on + strand, within BT3001 at 3782.982 kb on + strand, within BT3001 at 3782.982 kb on + strand, within BT3001 at 3782.982 kb on + strand, within BT3001 at 3782.982 kb on + strand, within BT3001 at 3783.007 kb on - strand, within BT3001 at 3783.013 kb on - strand, within BT3001 at 3783.019 kb on + strand, within BT3001 at 3783.019 kb on + strand, within BT3001 at 3783.033 kb on + strand, within BT3001 at 3783.055 kb on + strand, within BT3001 at 3783.082 kb on + strand, within BT3001 at 3783.121 kb on + strand, within BT3001 at 3783.130 kb on + strand, within BT3001 at 3783.130 kb on + strand, within BT3001 at 3783.156 kb on - strand, within BT3001 at 3783.203 kb on - strand, within BT3001 at 3783.205 kb on - strand, within BT3001 at 3783.234 kb on + strand, within BT3001 at 3783.334 kb on - strand, within BT3001 at 3783.334 kb on - strand, within BT3001
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 3,780,733 + BT2999 0.68 -0.2 3,780,733 + BT2999 0.68 -0.8 3,780,735 + BT2999 0.68 +0.3 3,780,735 + BT2999 0.68 -1.1 3,780,736 - BT2999 0.68 +2.8 3,780,756 - BT2999 0.69 -2.6 3,780,802 + BT2999 0.71 +1.8 3,780,807 - BT2999 0.71 +2.0 3,780,811 + BT2999 0.71 -1.4 3,780,811 + BT2999 0.71 +0.8 3,780,872 + BT2999 0.74 +3.6 3,780,873 - BT2999 0.74 -0.3 3,780,873 - BT2999 0.74 +0.5 3,780,961 - BT2999 0.77 -2.6 3,780,973 + BT2999 0.78 -1.6 3,780,984 - BT2999 0.78 -1.0 3,780,993 - BT2999 0.78 -0.2 3,781,004 + BT2999 0.79 -2.3 3,781,004 + BT2999 0.79 +2.2 3,781,029 + BT2999 0.80 -2.6 3,781,030 - BT2999 0.80 -0.8 3,781,048 - BT2999 0.80 -0.8 3,781,063 - BT2999 0.81 +1.0 3,781,082 + BT2999 0.82 +1.4 3,781,092 + BT2999 0.82 -1.3 3,781,096 - BT2999 0.82 -2.8 3,781,099 + BT2999 0.82 +1.8 3,781,102 + BT2999 0.82 +0.2 3,781,103 - BT2999 0.83 -1.7 3,781,103 - BT2999 0.83 -0.6 3,781,161 + BT2999 0.85 -2.7 3,781,161 + BT2999 0.85 -3.4 3,781,175 - BT2999 0.85 -0.4 3,781,185 - BT2999 0.86 -0.3 3,781,191 - BT2999 0.86 +0.2 3,781,194 + BT2999 0.86 -2.2 3,781,214 - BT2999 0.87 +2.2 3,781,214 - BT2999 0.87 -2.2 3,781,232 + BT2999 0.87 +1.9 3,781,236 + BT2999 0.88 +0.5 3,781,237 - BT2999 0.88 +0.8 3,781,237 - BT2999 0.88 +0.2 3,781,248 - +0.2 3,781,291 - BT2999 0.90 +0.2 3,781,330 + +1.1 3,781,355 - +1.5 3,781,360 + +1.1 3,781,360 + +2.1 3,781,402 - -1.9 3,781,402 - -1.3 3,781,469 + -0.7 3,781,478 + +0.1 3,781,486 + +2.3 3,781,491 + +0.2 3,781,550 + -2.1 3,781,567 - +1.4 3,781,599 + -0.8 3,781,600 - -0.7 3,781,600 - +3.2 3,781,617 + -0.2 3,781,620 + -0.4 3,781,620 + -2.0 3,781,621 - -1.0 3,781,626 - -1.4 3,781,626 - +0.4 3,781,626 - -2.8 3,781,685 - +0.9 3,781,711 + -0.9 3,781,711 + +3.8 3,781,711 + -1.5 3,781,722 + -1.0 3,781,722 + +1.2 3,781,722 + -2.1 3,781,722 + -1.4 3,781,722 + -1.3 3,781,723 - -3.4 3,781,723 - -0.8 3,781,724 + -1.4 3,781,724 + -0.1 3,781,724 + +1.2 3,781,724 + -1.2 3,781,725 - +2.2 3,781,725 - -1.5 3,781,726 + +3.2 3,781,811 + BT3000 0.12 +0.2 3,781,812 - BT3000 0.13 -0.8 3,781,812 - BT3000 0.13 -0.6 3,781,813 + BT3000 0.13 +3.3 3,781,826 - BT3000 0.15 +1.2 3,781,835 + BT3000 0.16 -0.0 3,781,879 - BT3000 0.23 +1.4 3,781,888 + BT3000 0.25 -0.6 3,781,889 - BT3000 0.25 -0.2 3,782,224 + BT3000 0.78 +4.3 3,782,254 + BT3000 0.83 +0.0 3,782,254 + BT3000 0.83 +2.0 3,782,256 + BT3000 0.83 -0.8 3,782,257 - BT3000 0.83 -1.2 3,782,257 - BT3000 0.83 +0.4 3,782,258 + BT3000 0.83 +0.9 3,782,259 - BT3000 0.83 -1.3 3,782,309 + -1.4 3,782,343 + -2.1 3,782,383 + +1.6 3,782,384 - -0.1 3,782,388 + -1.1 3,782,468 + +4.2 3,782,469 - -4.0 3,782,469 - -1.8 3,782,470 + -1.6 3,782,560 + -0.7 3,782,574 - +1.8 3,782,574 - +0.9 3,782,574 - +2.5 3,782,574 - -3.7 3,782,574 - -2.8 3,782,592 + -0.5 3,782,592 + -1.4 3,782,592 + +2.2 3,782,593 - -0.7 3,782,593 - -1.4 3,782,597 + -2.4 3,782,608 + +3.6 3,782,634 + +1.8 3,782,635 - -1.4 3,782,657 - BT3001 0.11 +0.6 3,782,659 + BT3001 0.11 +2.4 3,782,661 + BT3001 0.11 -0.2 3,782,662 - BT3001 0.12 -1.8 3,782,685 + BT3001 0.13 -0.8 3,782,692 + BT3001 0.13 -1.3 3,782,692 + BT3001 0.13 +1.2 3,782,693 - BT3001 0.14 -1.9 3,782,693 - BT3001 0.14 -2.7 3,782,735 - BT3001 0.16 +2.2 3,782,754 + BT3001 0.17 +0.2 3,782,755 - BT3001 0.17 -2.1 3,782,801 - BT3001 0.20 +1.2 3,782,833 - BT3001 0.22 -1.6 3,782,837 - BT3001 0.23 +0.2 3,782,837 - BT3001 0.23 +2.0 3,782,837 - BT3001 0.23 -0.5 3,782,844 + BT3001 0.23 +3.2 3,782,845 - BT3001 0.23 +0.8 3,782,851 - +0.2 3,782,853 + BT3001 0.24 -2.2 3,782,881 - BT3001 0.25 -1.2 3,782,926 - BT3001 0.28 +1.8 3,782,945 + BT3001 0.29 -0.6 3,782,982 + BT3001 0.32 -2.4 3,782,982 + BT3001 0.32 +0.6 3,782,982 + BT3001 0.32 +2.8 3,782,982 + BT3001 0.32 -0.6 3,783,007 - BT3001 0.33 -1.3 3,783,013 - BT3001 0.34 +1.7 3,783,019 + BT3001 0.34 -1.1 3,783,019 + BT3001 0.34 -3.4 3,783,033 + BT3001 0.35 -0.7 3,783,055 + BT3001 0.36 +1.2 3,783,082 + BT3001 0.38 +0.2 3,783,121 + BT3001 0.41 +1.5 3,783,130 + BT3001 0.41 -4.0 3,783,130 + BT3001 0.41 +0.6 3,783,156 - BT3001 0.43 -0.4 3,783,203 - BT3001 0.46 -1.1 3,783,205 - BT3001 0.46 +1.7 3,783,234 + BT3001 0.48 +2.3 3,783,334 - BT3001 0.54 -0.4 3,783,334 - BT3001 0.54 -1.4
Or see this region's nucleotide sequence