Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2869 and BT2870 overlap by 4 nucleotides BT2870 and BT2871 overlap by 1 nucleotides BT2871 and BT2872 overlap by 8 nucleotides BT2872 and BT2873 are separated by 11 nucleotides
BT2869: BT2869 - putative succinyltransferase involved in succinoglycan biosynthesis (NCBI ptt file), at 3,589,800 to 3,590,903
BT2869
BT2870: BT2870 - putative glycosyltransferase (NCBI ptt file), at 3,590,900 to 3,591,766
BT2870
BT2871: BT2871 - putative glycosyltransferase (NCBI ptt file), at 3,591,766 to 3,592,641
BT2871
BT2872: BT2872 - putative capsular polysaccharide synthesis protein (NCBI ptt file), at 3,592,634 to 3,593,563
BT2872
BT2873: BT2873 - putative stress protein (NCBI ptt file), at 3,593,575 to 3,594,504
BT2873
Position (kb)
3591
3592
3593 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3590.767 kb on - strand, within BT2869 at 3590.795 kb on + strand at 3590.838 kb on + strand at 3590.991 kb on - strand, within BT2870 at 3591.028 kb on + strand, within BT2870 at 3591.076 kb on + strand, within BT2870 at 3591.082 kb on - strand, within BT2870 at 3591.128 kb on + strand, within BT2870 at 3591.155 kb on - strand, within BT2870 at 3591.167 kb on + strand, within BT2870 at 3591.167 kb on + strand, within BT2870 at 3591.211 kb on + strand, within BT2870 at 3591.214 kb on + strand, within BT2870 at 3591.218 kb on + strand, within BT2870 at 3591.230 kb on - strand, within BT2870 at 3591.272 kb on + strand, within BT2870 at 3591.317 kb on + strand at 3591.371 kb on - strand, within BT2870 at 3591.371 kb on - strand, within BT2870 at 3591.485 kb on - strand, within BT2870 at 3591.510 kb on + strand, within BT2870 at 3591.515 kb on - strand, within BT2870 at 3591.515 kb on - strand, within BT2870 at 3591.566 kb on + strand, within BT2870 at 3591.566 kb on + strand, within BT2870 at 3591.567 kb on - strand, within BT2870 at 3591.572 kb on + strand, within BT2870 at 3591.572 kb on + strand, within BT2870 at 3591.572 kb on + strand, within BT2870 at 3591.573 kb on - strand, within BT2870 at 3591.573 kb on - strand, within BT2870 at 3591.573 kb on - strand, within BT2870 at 3591.575 kb on - strand, within BT2870 at 3591.595 kb on + strand, within BT2870 at 3591.596 kb on - strand, within BT2870 at 3591.675 kb on + strand, within BT2870 at 3591.675 kb on + strand, within BT2870 at 3591.675 kb on + strand, within BT2870 at 3591.675 kb on + strand, within BT2870 at 3591.676 kb on - strand, within BT2870 at 3591.676 kb on - strand, within BT2870 at 3591.737 kb on - strand at 3591.744 kb on - strand at 3591.744 kb on - strand at 3591.744 kb on - strand at 3591.754 kb on - strand at 3591.810 kb on + strand at 3591.811 kb on - strand at 3591.824 kb on + strand at 3591.828 kb on + strand at 3591.887 kb on + strand, within BT2871 at 3591.887 kb on + strand, within BT2871 at 3591.891 kb on + strand, within BT2871 at 3591.933 kb on + strand, within BT2871 at 3591.941 kb on + strand, within BT2871 at 3591.945 kb on - strand at 3591.945 kb on - strand, within BT2871 at 3591.949 kb on - strand, within BT2871 at 3591.958 kb on + strand, within BT2871 at 3592.069 kb on - strand, within BT2871 at 3592.069 kb on - strand, within BT2871 at 3592.069 kb on - strand, within BT2871 at 3592.075 kb on + strand, within BT2871 at 3592.076 kb on - strand, within BT2871 at 3592.076 kb on - strand, within BT2871 at 3592.098 kb on + strand, within BT2871 at 3592.101 kb on + strand, within BT2871 at 3592.101 kb on + strand, within BT2871 at 3592.129 kb on - strand, within BT2871 at 3592.129 kb on - strand, within BT2871 at 3592.156 kb on + strand, within BT2871 at 3592.156 kb on + strand, within BT2871 at 3592.169 kb on - strand, within BT2871 at 3592.186 kb on + strand, within BT2871 at 3592.200 kb on + strand, within BT2871 at 3592.201 kb on - strand, within BT2871 at 3592.207 kb on - strand, within BT2871 at 3592.207 kb on - strand, within BT2871 at 3592.218 kb on + strand, within BT2871 at 3592.228 kb on - strand, within BT2871 at 3592.230 kb on - strand, within BT2871 at 3592.243 kb on - strand, within BT2871 at 3592.282 kb on + strand, within BT2871 at 3592.291 kb on - strand, within BT2871 at 3592.322 kb on + strand, within BT2871 at 3592.322 kb on + strand, within BT2871 at 3592.376 kb on + strand, within BT2871 at 3592.412 kb on + strand, within BT2871 at 3592.413 kb on - strand, within BT2871 at 3592.413 kb on - strand, within BT2871 at 3592.423 kb on + strand, within BT2871 at 3592.482 kb on + strand, within BT2871 at 3592.506 kb on - strand, within BT2871 at 3592.533 kb on + strand, within BT2871 at 3592.534 kb on - strand, within BT2871 at 3592.535 kb on + strand, within BT2871 at 3592.535 kb on + strand, within BT2871 at 3592.535 kb on + strand, within BT2871 at 3592.535 kb on + strand, within BT2871 at 3592.536 kb on - strand, within BT2871 at 3592.536 kb on - strand, within BT2871 at 3592.579 kb on + strand at 3592.613 kb on + strand at 3592.631 kb on + strand at 3592.712 kb on - strand at 3592.850 kb on + strand, within BT2872 at 3592.961 kb on - strand, within BT2872 at 3592.963 kb on + strand, within BT2872 at 3592.964 kb on - strand, within BT2872 at 3592.964 kb on - strand, within BT2872 at 3592.964 kb on - strand, within BT2872 at 3592.981 kb on + strand, within BT2872 at 3593.014 kb on - strand, within BT2872 at 3593.062 kb on - strand, within BT2872 at 3593.112 kb on - strand, within BT2872 at 3593.119 kb on + strand at 3593.132 kb on + strand, within BT2872 at 3593.132 kb on + strand, within BT2872 at 3593.142 kb on + strand, within BT2872 at 3593.142 kb on + strand, within BT2872 at 3593.153 kb on + strand, within BT2872 at 3593.199 kb on - strand, within BT2872 at 3593.238 kb on - strand, within BT2872 at 3593.240 kb on - strand, within BT2872 at 3593.241 kb on + strand, within BT2872 at 3593.250 kb on - strand, within BT2872 at 3593.255 kb on + strand, within BT2872 at 3593.255 kb on + strand, within BT2872 at 3593.255 kb on + strand, within BT2872 at 3593.255 kb on + strand, within BT2872 at 3593.256 kb on - strand, within BT2872 at 3593.256 kb on - strand, within BT2872 at 3593.256 kb on - strand, within BT2872 at 3593.323 kb on - strand, within BT2872 at 3593.416 kb on - strand, within BT2872 at 3593.423 kb on + strand, within BT2872 at 3593.437 kb on - strand, within BT2872 at 3593.439 kb on + strand, within BT2872 at 3593.440 kb on - strand, within BT2872 at 3593.446 kb on + strand, within BT2872 at 3593.452 kb on + strand, within BT2872 at 3593.495 kb on - strand at 3593.495 kb on - strand at 3593.533 kb on + strand at 3593.546 kb on + strand at 3593.546 kb on + strand at 3593.547 kb on - strand at 3593.596 kb on + strand at 3593.596 kb on + strand at 3593.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 3,590,767 - BT2869 0.88 +0.1 3,590,795 + +1.9 3,590,838 + -2.2 3,590,991 - BT2870 0.10 +0.2 3,591,028 + BT2870 0.15 +2.1 3,591,076 + BT2870 0.20 +0.1 3,591,082 - BT2870 0.21 +1.1 3,591,128 + BT2870 0.26 +3.3 3,591,155 - BT2870 0.29 -1.8 3,591,167 + BT2870 0.31 -2.3 3,591,167 + BT2870 0.31 -0.7 3,591,211 + BT2870 0.36 -0.2 3,591,214 + BT2870 0.36 +1.7 3,591,218 + BT2870 0.37 -2.4 3,591,230 - BT2870 0.38 -3.6 3,591,272 + BT2870 0.43 -1.7 3,591,317 + -0.9 3,591,371 - BT2870 0.54 -1.7 3,591,371 - BT2870 0.54 +1.1 3,591,485 - BT2870 0.67 +1.1 3,591,510 + BT2870 0.70 +4.2 3,591,515 - BT2870 0.71 -0.4 3,591,515 - BT2870 0.71 +1.7 3,591,566 + BT2870 0.77 -2.8 3,591,566 + BT2870 0.77 +0.7 3,591,567 - BT2870 0.77 +0.6 3,591,572 + BT2870 0.78 -1.5 3,591,572 + BT2870 0.78 +0.7 3,591,572 + BT2870 0.78 -2.2 3,591,573 - BT2870 0.78 +1.9 3,591,573 - BT2870 0.78 +1.1 3,591,573 - BT2870 0.78 +0.6 3,591,575 - BT2870 0.78 +0.9 3,591,595 + BT2870 0.80 -2.4 3,591,596 - BT2870 0.80 -0.5 3,591,675 + BT2870 0.89 -0.7 3,591,675 + BT2870 0.89 +3.0 3,591,675 + BT2870 0.89 +1.9 3,591,675 + BT2870 0.89 -5.1 3,591,676 - BT2870 0.90 -0.9 3,591,676 - BT2870 0.90 -2.0 3,591,737 - -2.2 3,591,744 - +1.1 3,591,744 - +1.3 3,591,744 - +0.9 3,591,754 - -0.9 3,591,810 + +2.1 3,591,811 - +1.1 3,591,824 + -0.6 3,591,828 + -0.8 3,591,887 + BT2871 0.14 +1.7 3,591,887 + BT2871 0.14 +1.4 3,591,891 + BT2871 0.14 -0.9 3,591,933 + BT2871 0.19 -1.3 3,591,941 + BT2871 0.20 -1.3 3,591,945 - +0.1 3,591,945 - BT2871 0.20 -0.3 3,591,949 - BT2871 0.21 -0.8 3,591,958 + BT2871 0.22 -1.6 3,592,069 - BT2871 0.35 +1.3 3,592,069 - BT2871 0.35 +1.2 3,592,069 - BT2871 0.35 +1.1 3,592,075 + BT2871 0.35 +1.4 3,592,076 - BT2871 0.35 +0.1 3,592,076 - BT2871 0.35 +0.1 3,592,098 + BT2871 0.38 +1.9 3,592,101 + BT2871 0.38 -1.3 3,592,101 + BT2871 0.38 -1.0 3,592,129 - BT2871 0.41 +0.4 3,592,129 - BT2871 0.41 +3.1 3,592,156 + BT2871 0.45 +0.1 3,592,156 + BT2871 0.45 -2.2 3,592,169 - BT2871 0.46 +0.1 3,592,186 + BT2871 0.48 +2.9 3,592,200 + BT2871 0.50 -1.0 3,592,201 - BT2871 0.50 +0.7 3,592,207 - BT2871 0.50 +1.7 3,592,207 - BT2871 0.50 +2.1 3,592,218 + BT2871 0.52 -0.8 3,592,228 - BT2871 0.53 -2.2 3,592,230 - BT2871 0.53 +0.9 3,592,243 - BT2871 0.54 +0.6 3,592,282 + BT2871 0.59 -4.9 3,592,291 - BT2871 0.60 -0.9 3,592,322 + BT2871 0.63 +0.1 3,592,322 + BT2871 0.63 +1.3 3,592,376 + BT2871 0.70 -0.1 3,592,412 + BT2871 0.74 +2.7 3,592,413 - BT2871 0.74 -1.0 3,592,413 - BT2871 0.74 -2.6 3,592,423 + BT2871 0.75 +0.4 3,592,482 + BT2871 0.82 +0.4 3,592,506 - BT2871 0.84 -1.4 3,592,533 + BT2871 0.88 +3.1 3,592,534 - BT2871 0.88 -3.2 3,592,535 + BT2871 0.88 -1.1 3,592,535 + BT2871 0.88 -2.4 3,592,535 + BT2871 0.88 -2.4 3,592,535 + BT2871 0.88 +0.0 3,592,536 - BT2871 0.88 -2.0 3,592,536 - BT2871 0.88 -3.3 3,592,579 + +1.1 3,592,613 + -2.9 3,592,631 + +0.1 3,592,712 - -1.6 3,592,850 + BT2872 0.23 +2.1 3,592,961 - BT2872 0.35 +1.5 3,592,963 + BT2872 0.35 -0.9 3,592,964 - BT2872 0.35 -0.6 3,592,964 - BT2872 0.35 -0.9 3,592,964 - BT2872 0.35 +2.4 3,592,981 + BT2872 0.37 +0.1 3,593,014 - BT2872 0.41 +0.5 3,593,062 - BT2872 0.46 -1.4 3,593,112 - BT2872 0.51 +0.1 3,593,119 + +0.1 3,593,132 + BT2872 0.54 -0.4 3,593,132 + BT2872 0.54 -1.5 3,593,142 + BT2872 0.55 -3.6 3,593,142 + BT2872 0.55 -1.1 3,593,153 + BT2872 0.56 -1.0 3,593,199 - BT2872 0.61 -0.9 3,593,238 - BT2872 0.65 +3.3 3,593,240 - BT2872 0.65 -1.0 3,593,241 + BT2872 0.65 -0.4 3,593,250 - BT2872 0.66 -0.2 3,593,255 + BT2872 0.67 -0.5 3,593,255 + BT2872 0.67 +0.7 3,593,255 + BT2872 0.67 -1.1 3,593,255 + BT2872 0.67 -0.9 3,593,256 - BT2872 0.67 -0.5 3,593,256 - BT2872 0.67 -1.7 3,593,256 - BT2872 0.67 -3.9 3,593,323 - BT2872 0.74 -1.4 3,593,416 - BT2872 0.84 +0.1 3,593,423 + BT2872 0.85 -3.5 3,593,437 - BT2872 0.86 +1.1 3,593,439 + BT2872 0.87 +0.7 3,593,440 - BT2872 0.87 -1.9 3,593,446 + BT2872 0.87 +0.1 3,593,452 + BT2872 0.88 -0.9 3,593,495 - -3.3 3,593,495 - +1.1 3,593,533 + -0.4 3,593,546 + -1.4 3,593,546 + +0.4 3,593,547 - -0.5 3,593,596 + -2.9 3,593,596 + +4.0 3,593,611 - -1.4
Or see this region's nucleotide sequence