Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1724 and BT1725 are separated by 10 nucleotides BT1725 and BT1726 are separated by 336 nucleotides BT1726 and BT1727 are separated by 568 nucleotides
BT1724: BT1724 - conserved hypothetical protein (NCBI ptt file), at 2,123,274 to 2,123,642
BT1724
BT1725: BT1725 - putative transcriptional regulator (NCBI ptt file), at 2,123,653 to 2,124,231
BT1725
BT1726: BT1726 - integrase (NCBI ptt file), at 2,124,568 to 2,125,518
BT1726
BT1727: BT1727 - putative transmembrane sensor (NCBI ptt file), at 2,126,087 to 2,126,938
BT1727
Position (kb)
2124
2125
2126 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2123.633 kb on + strand at 2123.695 kb on - strand at 2123.993 kb on - strand, within BT1725 at 2124.845 kb on + strand, within BT1726 at 2125.272 kb on - strand, within BT1726 at 2125.276 kb on - strand, within BT1726 at 2125.288 kb on - strand, within BT1726 at 2125.294 kb on - strand, within BT1726 at 2125.294 kb on - strand, within BT1726 at 2125.296 kb on - strand, within BT1726 at 2125.390 kb on - strand, within BT1726 at 2125.512 kb on + strand at 2125.523 kb on + strand at 2125.523 kb on + strand at 2125.531 kb on + strand at 2125.554 kb on + strand at 2125.572 kb on + strand at 2125.597 kb on - strand at 2125.602 kb on - strand at 2125.701 kb on - strand at 2125.701 kb on - strand at 2125.711 kb on - strand at 2125.713 kb on + strand at 2125.732 kb on + strand at 2125.797 kb on - strand at 2125.806 kb on - strand at 2125.818 kb on - strand at 2125.876 kb on + strand at 2125.880 kb on + strand at 2125.881 kb on - strand at 2125.906 kb on - strand at 2126.039 kb on - strand at 2126.040 kb on + strand at 2126.050 kb on - strand at 2126.093 kb on + strand at 2126.197 kb on - strand, within BT1727 at 2126.197 kb on - strand, within BT1727 at 2126.223 kb on - strand, within BT1727 at 2126.223 kb on - strand, within BT1727 at 2126.223 kb on - strand, within BT1727 at 2126.243 kb on - strand, within BT1727 at 2126.262 kb on - strand, within BT1727 at 2126.295 kb on - strand, within BT1727 at 2126.336 kb on - strand, within BT1727 at 2126.397 kb on - strand, within BT1727 at 2126.499 kb on - strand, within BT1727
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 2,123,633 + +2.0 2,123,695 - -1.0 2,123,993 - BT1725 0.59 -1.0 2,124,845 + BT1726 0.29 +1.0 2,125,272 - BT1726 0.74 -2.1 2,125,276 - BT1726 0.74 -2.0 2,125,288 - BT1726 0.76 -2.3 2,125,294 - BT1726 0.76 -1.0 2,125,294 - BT1726 0.76 +0.0 2,125,296 - BT1726 0.77 -0.2 2,125,390 - BT1726 0.86 -2.0 2,125,512 + -0.2 2,125,523 + -0.6 2,125,523 + +0.7 2,125,531 + -0.6 2,125,554 + -0.3 2,125,572 + +1.0 2,125,597 - +1.0 2,125,602 - -2.7 2,125,701 - +0.8 2,125,701 - +2.0 2,125,711 - -2.0 2,125,713 + -0.5 2,125,732 + +0.9 2,125,797 - -0.4 2,125,806 - -1.9 2,125,818 - -3.1 2,125,876 + +0.9 2,125,880 + -2.0 2,125,881 - -1.0 2,125,906 - -1.3 2,126,039 - +2.9 2,126,040 + +1.3 2,126,050 - -0.4 2,126,093 + +0.0 2,126,197 - BT1727 0.13 +2.3 2,126,197 - BT1727 0.13 +1.1 2,126,223 - BT1727 0.16 -2.0 2,126,223 - BT1727 0.16 -3.1 2,126,223 - BT1727 0.16 +0.0 2,126,243 - BT1727 0.18 +0.0 2,126,262 - BT1727 0.21 +1.3 2,126,295 - BT1727 0.24 +0.3 2,126,336 - BT1727 0.29 -1.4 2,126,397 - BT1727 0.36 -0.4 2,126,499 - BT1727 0.48 +1.6
Or see this region's nucleotide sequence