Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1537 and BT1538 are separated by 54 nucleotides BT1538 and BT1539 are separated by 198 nucleotides BT1539 and BT1540 are separated by 3 nucleotides
BT1537: BT1537 - putative NADH dehydrogenase (NCBI ptt file), at 1,896,554 to 1,897,945
BT1537
BT1538: BT1538 - hemagglutinin (NCBI ptt file), at 1,898,000 to 1,898,905
BT1538
BT1539: BT1539 - cytidine deaminase (NCBI ptt file), at 1,899,104 to 1,899,499
BT1539
BT1540: BT1540 - transcriptional regulator (NCBI ptt file), at 1,899,503 to 1,900,342
BT1540
Position (kb)
1897
1898
1899 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1897.025 kb on + strand, within BT1537 at 1897.043 kb on + strand, within BT1537 at 1897.159 kb on + strand, within BT1537 at 1897.165 kb on + strand, within BT1537 at 1897.339 kb on + strand, within BT1537 at 1897.339 kb on + strand, within BT1537 at 1897.340 kb on - strand, within BT1537 at 1897.340 kb on - strand, within BT1537 at 1897.443 kb on - strand, within BT1537 at 1897.537 kb on + strand, within BT1537 at 1897.580 kb on - strand, within BT1537 at 1897.586 kb on - strand, within BT1537 at 1897.629 kb on + strand, within BT1537 at 1897.630 kb on - strand, within BT1537 at 1897.692 kb on - strand, within BT1537 at 1897.710 kb on + strand, within BT1537 at 1897.771 kb on + strand, within BT1537 at 1897.825 kb on + strand at 1897.826 kb on - strand at 1897.933 kb on + strand at 1897.961 kb on + strand at 1897.967 kb on - strand at 1897.984 kb on + strand at 1897.984 kb on + strand at 1898.001 kb on + strand at 1898.111 kb on - strand, within BT1538 at 1898.133 kb on + strand, within BT1538 at 1898.186 kb on - strand, within BT1538 at 1898.914 kb on - strand at 1898.987 kb on + strand at 1899.053 kb on + strand at 1899.053 kb on + strand at 1899.053 kb on + strand at 1899.125 kb on + strand at 1899.242 kb on - strand, within BT1539 at 1899.254 kb on + strand, within BT1539 at 1899.328 kb on - strand, within BT1539 at 1899.328 kb on - strand, within BT1539 at 1899.389 kb on + strand, within BT1539 at 1899.431 kb on - strand, within BT1539 at 1899.435 kb on - strand, within BT1539 at 1899.596 kb on + strand, within BT1540 at 1899.604 kb on + strand, within BT1540 at 1899.657 kb on + strand, within BT1540 at 1899.657 kb on + strand, within BT1540 at 1899.658 kb on - strand, within BT1540 at 1899.729 kb on - strand, within BT1540
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,897,025 + BT1537 0.34 -3.3 1,897,043 + BT1537 0.35 -2.2 1,897,159 + BT1537 0.43 -2.1 1,897,165 + BT1537 0.44 -2.9 1,897,339 + BT1537 0.56 -0.1 1,897,339 + BT1537 0.56 -1.1 1,897,340 - BT1537 0.56 -1.6 1,897,340 - BT1537 0.56 -2.1 1,897,443 - BT1537 0.64 -1.6 1,897,537 + BT1537 0.71 -4.1 1,897,580 - BT1537 0.74 -1.8 1,897,586 - BT1537 0.74 -2.1 1,897,629 + BT1537 0.77 -1.1 1,897,630 - BT1537 0.77 -1.1 1,897,692 - BT1537 0.82 -3.5 1,897,710 + BT1537 0.83 -1.4 1,897,771 + BT1537 0.87 -1.1 1,897,825 + -1.6 1,897,826 - -0.1 1,897,933 + -0.1 1,897,961 + -1.1 1,897,967 - -2.6 1,897,984 + -1.6 1,897,984 + -2.4 1,898,001 + -1.1 1,898,111 - BT1538 0.12 -1.1 1,898,133 + BT1538 0.15 -1.6 1,898,186 - BT1538 0.21 -0.1 1,898,914 - -1.6 1,898,987 + +1.9 1,899,053 + -3.6 1,899,053 + -2.1 1,899,053 + +0.7 1,899,125 + -3.1 1,899,242 - BT1539 0.35 -1.1 1,899,254 + BT1539 0.38 -3.4 1,899,328 - BT1539 0.57 -0.1 1,899,328 - BT1539 0.57 -1.4 1,899,389 + BT1539 0.72 -2.9 1,899,431 - BT1539 0.83 -1.5 1,899,435 - BT1539 0.84 -0.1 1,899,596 + BT1540 0.11 +0.2 1,899,604 + BT1540 0.12 -4.1 1,899,657 + BT1540 0.18 +1.9 1,899,657 + BT1540 0.18 -2.4 1,899,658 - BT1540 0.18 +0.5 1,899,729 - BT1540 0.27 +0.5
Or see this region's nucleotide sequence