Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1472 and BT1473 are separated by 11 nucleotides BT1473 and BT1474 overlap by 1 nucleotides
BT1472: BT1472 - conserved hypothetical protein (NCBI ptt file), at 1,813,936 to 1,815,108
BT1472
BT1473: BT1473 - conserved hypothetical protein (NCBI ptt file), at 1,815,120 to 1,815,575
BT1473
BT1474: BT1474 - ribosomal protein S6 modification protein-related protein (NCBI ptt file), at 1,815,575 to 1,817,041
BT1474
Position (kb)
1815
1816 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1814.176 kb on + strand, within BT1472 at 1814.234 kb on + strand, within BT1472 at 1814.234 kb on + strand, within BT1472 at 1814.235 kb on - strand, within BT1472 at 1814.368 kb on - strand, within BT1472 at 1814.464 kb on + strand, within BT1472 at 1814.498 kb on + strand, within BT1472 at 1814.560 kb on + strand, within BT1472 at 1814.561 kb on - strand, within BT1472 at 1814.661 kb on - strand, within BT1472 at 1814.712 kb on - strand, within BT1472 at 1814.721 kb on + strand, within BT1472 at 1814.729 kb on + strand, within BT1472 at 1814.730 kb on - strand, within BT1472 at 1814.771 kb on + strand, within BT1472 at 1814.777 kb on - strand, within BT1472 at 1814.777 kb on - strand, within BT1472 at 1814.815 kb on + strand, within BT1472 at 1814.952 kb on - strand, within BT1472 at 1815.163 kb on + strand at 1815.166 kb on + strand, within BT1473 at 1815.209 kb on - strand, within BT1473 at 1815.211 kb on - strand, within BT1473 at 1815.228 kb on - strand at 1815.266 kb on + strand, within BT1473 at 1815.558 kb on + strand at 1815.559 kb on - strand at 1815.576 kb on + strand at 1815.627 kb on + strand at 1815.801 kb on + strand, within BT1474 at 1815.806 kb on - strand, within BT1474 at 1815.806 kb on - strand, within BT1474 at 1815.883 kb on + strand, within BT1474 at 1815.884 kb on - strand, within BT1474 at 1815.885 kb on + strand, within BT1474 at 1815.888 kb on + strand at 1815.888 kb on + strand, within BT1474 at 1815.900 kb on + strand, within BT1474 at 1815.900 kb on + strand, within BT1474 at 1815.901 kb on - strand, within BT1474 at 1815.908 kb on + strand, within BT1474 at 1815.908 kb on + strand, within BT1474 at 1816.051 kb on - strand, within BT1474 at 1816.141 kb on + strand, within BT1474 at 1816.142 kb on - strand, within BT1474 at 1816.195 kb on + strand, within BT1474 at 1816.277 kb on - strand, within BT1474
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,814,176 + BT1472 0.20 +2.7 1,814,234 + BT1472 0.25 -1.1 1,814,234 + BT1472 0.25 -3.7 1,814,235 - BT1472 0.25 +1.1 1,814,368 - BT1472 0.37 -0.1 1,814,464 + BT1472 0.45 -1.9 1,814,498 + BT1472 0.48 +2.7 1,814,560 + BT1472 0.53 +2.7 1,814,561 - BT1472 0.53 +2.6 1,814,661 - BT1472 0.62 -1.6 1,814,712 - BT1472 0.66 +2.5 1,814,721 + BT1472 0.67 -4.0 1,814,729 + BT1472 0.68 -3.7 1,814,730 - BT1472 0.68 +0.5 1,814,771 + BT1472 0.71 -0.1 1,814,777 - BT1472 0.72 -2.1 1,814,777 - BT1472 0.72 -0.1 1,814,815 + BT1472 0.75 -2.1 1,814,952 - BT1472 0.87 -0.1 1,815,163 + +1.7 1,815,166 + BT1473 0.10 +2.9 1,815,209 - BT1473 0.20 -2.3 1,815,211 - BT1473 0.20 -0.4 1,815,228 - -0.1 1,815,266 + BT1473 0.32 -3.5 1,815,558 + -1.6 1,815,559 - -0.1 1,815,576 + +0.1 1,815,627 + -0.1 1,815,801 + BT1474 0.15 +3.4 1,815,806 - BT1474 0.16 +3.1 1,815,806 - BT1474 0.16 -1.7 1,815,883 + BT1474 0.21 +1.9 1,815,884 - BT1474 0.21 -0.1 1,815,885 + BT1474 0.21 -1.1 1,815,888 + -1.7 1,815,888 + BT1474 0.21 -3.1 1,815,900 + BT1474 0.22 -2.4 1,815,900 + BT1474 0.22 -0.1 1,815,901 - BT1474 0.22 -2.0 1,815,908 + BT1474 0.23 +1.6 1,815,908 + BT1474 0.23 -0.1 1,816,051 - BT1474 0.32 +1.7 1,816,141 + BT1474 0.39 +1.2 1,816,142 - BT1474 0.39 -0.1 1,816,195 + BT1474 0.42 +1.8 1,816,277 - BT1474 0.48 +0.5
Or see this region's nucleotide sequence