Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1432 and BT1433 are separated by 49 nucleotides BT1433 and BT1434 are separated by 20 nucleotides BT1434 and BT1435 are separated by 470 nucleotides BT1435 and BT1436 are separated by 44 nucleotides
BT1432: BT1432 - mannonate dehydratase (NCBI ptt file), at 1,762,678 to 1,763,847
BT1432
BT1433: BT1433 - putative oxidoreductase (NCBI ptt file), at 1,763,897 to 1,764,709
BT1433
BT1434: BT1434 - transcriptional regulator, putative catabolite control protein A (NCBI ptt file), at 1,764,730 to 1,765,755
BT1434
BT1435: BT1435 - hypothetical protein (NCBI ptt file), at 1,766,226 to 1,766,513
BT1435
BT1436: BT1436 - hypothetical protein (NCBI ptt file), at 1,766,558 to 1,766,803
BT1436
Position (kb)
1764
1765
1766 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1763.756 kb on - strand at 1763.759 kb on - strand at 1763.851 kb on + strand at 1763.856 kb on + strand at 1763.861 kb on - strand at 1763.878 kb on - strand at 1763.884 kb on + strand at 1763.891 kb on + strand at 1763.895 kb on + strand at 1763.929 kb on + strand at 1763.941 kb on + strand at 1763.942 kb on - strand at 1763.957 kb on - strand at 1763.977 kb on - strand at 1764.132 kb on - strand, within BT1433 at 1764.197 kb on - strand, within BT1433 at 1764.290 kb on + strand, within BT1433 at 1764.291 kb on - strand, within BT1433 at 1764.314 kb on - strand, within BT1433 at 1764.419 kb on - strand, within BT1433 at 1764.421 kb on + strand, within BT1433 at 1764.422 kb on - strand, within BT1433 at 1764.452 kb on - strand, within BT1433 at 1764.460 kb on - strand, within BT1433 at 1764.529 kb on + strand, within BT1433 at 1764.572 kb on - strand, within BT1433 at 1764.608 kb on - strand, within BT1433 at 1764.619 kb on + strand, within BT1433 at 1764.620 kb on - strand, within BT1433 at 1764.627 kb on + strand, within BT1433 at 1764.628 kb on - strand at 1764.628 kb on - strand at 1764.644 kb on - strand at 1764.648 kb on - strand at 1764.698 kb on + strand at 1764.732 kb on - strand at 1764.749 kb on + strand at 1764.752 kb on + strand at 1764.855 kb on - strand, within BT1434 at 1764.883 kb on + strand, within BT1434 at 1764.883 kb on + strand, within BT1434 at 1765.027 kb on + strand, within BT1434 at 1765.029 kb on + strand, within BT1434 at 1765.030 kb on - strand, within BT1434 at 1765.100 kb on - strand, within BT1434 at 1765.100 kb on - strand, within BT1434 at 1765.278 kb on - strand, within BT1434 at 1765.380 kb on + strand, within BT1434 at 1765.421 kb on - strand, within BT1434 at 1765.468 kb on - strand, within BT1434 at 1765.473 kb on - strand, within BT1434 at 1765.502 kb on - strand, within BT1434 at 1765.504 kb on - strand, within BT1434 at 1765.506 kb on + strand, within BT1434 at 1765.551 kb on + strand, within BT1434 at 1765.588 kb on - strand, within BT1434 at 1765.808 kb on + strand at 1765.855 kb on - strand at 1765.940 kb on + strand at 1765.987 kb on + strand at 1766.060 kb on + strand at 1766.098 kb on + strand at 1766.113 kb on - strand at 1766.164 kb on - strand at 1766.186 kb on - strand at 1766.228 kb on - strand at 1766.286 kb on - strand, within BT1435 at 1766.298 kb on - strand, within BT1435 at 1766.397 kb on - strand, within BT1435 at 1766.399 kb on - strand, within BT1435 at 1766.403 kb on - strand, within BT1435 at 1766.452 kb on - strand, within BT1435 at 1766.452 kb on - strand, within BT1435 at 1766.505 kb on - strand at 1766.549 kb on - strand at 1766.550 kb on + strand at 1766.591 kb on + strand, within BT1436 at 1766.603 kb on - strand, within BT1436 at 1766.610 kb on - strand, within BT1436 at 1766.615 kb on - strand, within BT1436 at 1766.615 kb on - strand, within BT1436 at 1766.622 kb on - strand, within BT1436 at 1766.670 kb on - strand, within BT1436 at 1766.699 kb on - strand, within BT1436 at 1766.719 kb on + strand, within BT1436 at 1766.744 kb on + strand, within BT1436
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,763,756 - +0.3 1,763,759 - +1.7 1,763,851 + -1.1 1,763,856 + -1.5 1,763,861 - -0.1 1,763,878 - -2.7 1,763,884 + +2.2 1,763,891 + -0.7 1,763,895 + +0.9 1,763,929 + -1.7 1,763,941 + +2.4 1,763,942 - -0.1 1,763,957 - +1.4 1,763,977 - +3.3 1,764,132 - BT1433 0.29 -0.0 1,764,197 - BT1433 0.37 -2.5 1,764,290 + BT1433 0.48 -1.4 1,764,291 - BT1433 0.48 +0.1 1,764,314 - BT1433 0.51 -0.4 1,764,419 - BT1433 0.64 -1.3 1,764,421 + BT1433 0.64 +1.2 1,764,422 - BT1433 0.65 +0.9 1,764,452 - BT1433 0.68 +1.3 1,764,460 - BT1433 0.69 +0.9 1,764,529 + BT1433 0.78 +2.4 1,764,572 - BT1433 0.83 +1.9 1,764,608 - BT1433 0.87 -2.6 1,764,619 + BT1433 0.89 -2.2 1,764,620 - BT1433 0.89 -0.7 1,764,627 + BT1433 0.90 +0.2 1,764,628 - -1.9 1,764,628 - -1.1 1,764,644 - +0.9 1,764,648 - +1.9 1,764,698 + -0.6 1,764,732 - +0.9 1,764,749 + +1.4 1,764,752 + -0.3 1,764,855 - BT1434 0.12 -1.1 1,764,883 + BT1434 0.15 -1.7 1,764,883 + BT1434 0.15 -3.1 1,765,027 + BT1434 0.29 +1.1 1,765,029 + BT1434 0.29 -1.7 1,765,030 - BT1434 0.29 -1.1 1,765,100 - BT1434 0.36 -1.7 1,765,100 - BT1434 0.36 +1.4 1,765,278 - BT1434 0.53 +3.4 1,765,380 + BT1434 0.63 -1.6 1,765,421 - BT1434 0.67 -0.1 1,765,468 - BT1434 0.72 -0.1 1,765,473 - BT1434 0.72 -1.7 1,765,502 - BT1434 0.75 -2.7 1,765,504 - BT1434 0.75 -1.1 1,765,506 + BT1434 0.76 -1.1 1,765,551 + BT1434 0.80 +0.9 1,765,588 - BT1434 0.84 +0.0 1,765,808 + +0.3 1,765,855 - +0.9 1,765,940 + -2.1 1,765,987 + -0.1 1,766,060 + -0.1 1,766,098 + -0.3 1,766,113 - -2.7 1,766,164 - -3.8 1,766,186 - -0.7 1,766,228 - +2.6 1,766,286 - BT1435 0.21 +1.2 1,766,298 - BT1435 0.25 +1.9 1,766,397 - BT1435 0.59 -0.5 1,766,399 - BT1435 0.60 -1.1 1,766,403 - BT1435 0.61 -0.5 1,766,452 - BT1435 0.78 +1.9 1,766,452 - BT1435 0.78 -1.4 1,766,505 - -0.2 1,766,549 - +0.1 1,766,550 + -2.7 1,766,591 + BT1436 0.13 +0.8 1,766,603 - BT1436 0.18 -1.8 1,766,610 - BT1436 0.21 -0.8 1,766,615 - BT1436 0.23 -1.5 1,766,615 - BT1436 0.23 -0.7 1,766,622 - BT1436 0.26 +0.7 1,766,670 - BT1436 0.46 +2.9 1,766,699 - BT1436 0.57 -0.1 1,766,719 + BT1436 0.65 +1.4 1,766,744 + BT1436 0.76 -2.5
Or see this region's nucleotide sequence