Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1248 and BT1249 are separated by 5 nucleotides BT1249 and BT1250 are separated by 51 nucleotides
BT1248: BT1248 - putative transport protein (NCBI ptt file), at 1,554,128 to 1,555,723
BT1248
BT1249: BT1249 - putative multidrug resistance protein (NCBI ptt file), at 1,555,729 to 1,556,757
BT1249
BT1250: BT1250 - conserved hypothetical protein, putative outer membrane efflux protein (NCBI ptt file), at 1,556,809 to 1,558,128
BT1250
Position (kb)
1555
1556
1557 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1554.758 kb on + strand, within BT1248 at 1554.759 kb on - strand, within BT1248 at 1554.797 kb on - strand, within BT1248 at 1554.831 kb on + strand, within BT1248 at 1554.882 kb on + strand, within BT1248 at 1554.930 kb on - strand, within BT1248 at 1554.930 kb on - strand, within BT1248 at 1554.968 kb on + strand, within BT1248 at 1554.968 kb on + strand, within BT1248 at 1554.980 kb on + strand, within BT1248 at 1555.057 kb on - strand, within BT1248 at 1555.203 kb on + strand, within BT1248 at 1555.208 kb on - strand, within BT1248 at 1555.208 kb on - strand, within BT1248 at 1555.209 kb on + strand at 1555.210 kb on - strand, within BT1248 at 1555.278 kb on - strand, within BT1248 at 1555.284 kb on + strand, within BT1248 at 1555.296 kb on + strand, within BT1248 at 1555.508 kb on - strand, within BT1248 at 1555.520 kb on + strand, within BT1248 at 1555.554 kb on + strand, within BT1248 at 1555.566 kb on - strand at 1555.620 kb on + strand at 1555.651 kb on + strand at 1555.761 kb on + strand at 1555.797 kb on + strand at 1555.960 kb on + strand, within BT1249 at 1555.975 kb on - strand, within BT1249 at 1555.980 kb on + strand, within BT1249 at 1556.042 kb on + strand, within BT1249 at 1556.069 kb on + strand, within BT1249 at 1556.083 kb on + strand, within BT1249 at 1556.084 kb on - strand, within BT1249 at 1556.103 kb on - strand, within BT1249 at 1556.182 kb on - strand, within BT1249 at 1556.222 kb on + strand, within BT1249 at 1556.254 kb on + strand, within BT1249 at 1556.357 kb on + strand, within BT1249 at 1556.400 kb on + strand, within BT1249 at 1556.400 kb on + strand, within BT1249 at 1556.400 kb on + strand, within BT1249 at 1556.421 kb on + strand, within BT1249 at 1556.421 kb on + strand, within BT1249 at 1556.536 kb on + strand, within BT1249 at 1556.562 kb on - strand, within BT1249 at 1556.562 kb on - strand, within BT1249 at 1556.615 kb on - strand, within BT1249 at 1556.627 kb on + strand, within BT1249 at 1556.636 kb on - strand, within BT1249 at 1556.661 kb on - strand at 1556.664 kb on + strand at 1556.664 kb on + strand at 1556.685 kb on + strand at 1556.688 kb on + strand at 1556.803 kb on - strand at 1556.807 kb on + strand at 1556.808 kb on - strand at 1556.811 kb on - strand at 1556.847 kb on + strand at 1556.964 kb on + strand, within BT1250 at 1556.965 kb on - strand, within BT1250 at 1556.970 kb on - strand, within BT1250 at 1557.097 kb on - strand, within BT1250 at 1557.192 kb on + strand, within BT1250 at 1557.200 kb on + strand, within BT1250 at 1557.201 kb on - strand, within BT1250 at 1557.241 kb on - strand, within BT1250 at 1557.251 kb on + strand, within BT1250 at 1557.365 kb on + strand, within BT1250 at 1557.367 kb on - strand, within BT1250 at 1557.369 kb on - strand, within BT1250 at 1557.479 kb on - strand, within BT1250 at 1557.544 kb on + strand at 1557.631 kb on + strand, within BT1250 at 1557.631 kb on + strand, within BT1250
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,554,758 + BT1248 0.39 +0.8 1,554,759 - BT1248 0.40 +2.8 1,554,797 - BT1248 0.42 +2.1 1,554,831 + BT1248 0.44 -0.5 1,554,882 + BT1248 0.47 -0.9 1,554,930 - BT1248 0.50 +1.4 1,554,930 - BT1248 0.50 +0.8 1,554,968 + BT1248 0.53 -0.2 1,554,968 + BT1248 0.53 +1.5 1,554,980 + BT1248 0.53 +1.8 1,555,057 - BT1248 0.58 -2.1 1,555,203 + BT1248 0.67 -1.0 1,555,208 - BT1248 0.68 -1.2 1,555,208 - BT1248 0.68 +2.6 1,555,209 + -0.2 1,555,210 - BT1248 0.68 +0.6 1,555,278 - BT1248 0.72 -1.8 1,555,284 + BT1248 0.72 -0.5 1,555,296 + BT1248 0.73 -0.2 1,555,508 - BT1248 0.86 -0.2 1,555,520 + BT1248 0.87 +1.0 1,555,554 + BT1248 0.89 +2.4 1,555,566 - +0.8 1,555,620 + +2.4 1,555,651 + -0.2 1,555,761 + -1.8 1,555,797 + -2.5 1,555,960 + BT1249 0.22 -0.9 1,555,975 - BT1249 0.24 +0.3 1,555,980 + BT1249 0.24 +0.4 1,556,042 + BT1249 0.30 +1.2 1,556,069 + BT1249 0.33 +1.7 1,556,083 + BT1249 0.34 -0.2 1,556,084 - BT1249 0.34 -1.2 1,556,103 - BT1249 0.36 -0.4 1,556,182 - BT1249 0.44 -0.6 1,556,222 + BT1249 0.48 +1.6 1,556,254 + BT1249 0.51 -1.2 1,556,357 + BT1249 0.61 -0.2 1,556,400 + BT1249 0.65 +0.4 1,556,400 + BT1249 0.65 -0.8 1,556,400 + BT1249 0.65 +0.6 1,556,421 + BT1249 0.67 -1.8 1,556,421 + BT1249 0.67 +0.1 1,556,536 + BT1249 0.78 -0.3 1,556,562 - BT1249 0.81 +1.1 1,556,562 - BT1249 0.81 -0.5 1,556,615 - BT1249 0.86 +1.3 1,556,627 + BT1249 0.87 +1.5 1,556,636 - BT1249 0.88 +0.8 1,556,661 - +0.6 1,556,664 + +1.6 1,556,664 + -1.8 1,556,685 + -0.2 1,556,688 + -0.9 1,556,803 - -0.2 1,556,807 + -0.7 1,556,808 - -1.2 1,556,811 - +3.7 1,556,847 + +1.6 1,556,964 + BT1250 0.12 -3.5 1,556,965 - BT1250 0.12 -1.2 1,556,970 - BT1250 0.12 +1.1 1,557,097 - BT1250 0.22 -0.2 1,557,192 + BT1250 0.29 -2.4 1,557,200 + BT1250 0.30 -1.8 1,557,201 - BT1250 0.30 +2.0 1,557,241 - BT1250 0.33 +0.4 1,557,251 + BT1250 0.33 +0.5 1,557,365 + BT1250 0.42 -1.8 1,557,367 - BT1250 0.42 -1.8 1,557,369 - BT1250 0.42 +2.4 1,557,479 - BT1250 0.51 -0.9 1,557,544 + -1.8 1,557,631 + BT1250 0.62 +0.4 1,557,631 + BT1250 0.62 -2.6
Or see this region's nucleotide sequence