Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1180 and BT1181 are separated by 11 nucleotides BT1181 and BT1182 are separated by 24 nucleotides
BT1180: BT1180 - putative glycosyltransferase (NCBI ptt file), at 1,469,098 to 1,470,252
BT1180
BT1181: BT1181 - glycosyltransferase involved in cell wall biogenesis (NCBI ptt file), at 1,470,264 to 1,471,115
BT1181
BT1182: BT1182 - hypothetical protein (NCBI ptt file), at 1,471,140 to 1,472,642
BT1182
Position (kb)
1470
1471
1472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1469.279 kb on + strand, within BT1180 at 1469.280 kb on - strand, within BT1180 at 1469.319 kb on - strand, within BT1180 at 1469.323 kb on - strand, within BT1180 at 1469.403 kb on + strand, within BT1180 at 1469.607 kb on + strand, within BT1180 at 1469.608 kb on - strand, within BT1180 at 1469.608 kb on - strand, within BT1180 at 1469.701 kb on - strand, within BT1180 at 1469.713 kb on - strand, within BT1180 at 1469.719 kb on - strand, within BT1180 at 1469.762 kb on + strand, within BT1180 at 1469.819 kb on - strand, within BT1180 at 1470.003 kb on + strand, within BT1180 at 1470.004 kb on - strand, within BT1180 at 1470.004 kb on - strand, within BT1180 at 1470.051 kb on - strand, within BT1180 at 1470.264 kb on - strand at 1470.395 kb on - strand, within BT1181 at 1470.395 kb on - strand, within BT1181 at 1470.434 kb on + strand, within BT1181 at 1470.435 kb on - strand, within BT1181 at 1470.544 kb on - strand, within BT1181 at 1470.643 kb on - strand, within BT1181 at 1470.680 kb on - strand, within BT1181 at 1470.779 kb on + strand, within BT1181 at 1470.782 kb on - strand, within BT1181 at 1470.830 kb on + strand, within BT1181 at 1470.849 kb on - strand, within BT1181 at 1470.884 kb on + strand, within BT1181 at 1470.921 kb on - strand, within BT1181 at 1470.980 kb on + strand, within BT1181 at 1470.981 kb on - strand, within BT1181 at 1471.017 kb on - strand, within BT1181 at 1471.022 kb on - strand, within BT1181 at 1471.138 kb on - strand at 1471.149 kb on + strand at 1471.152 kb on - strand at 1471.157 kb on - strand at 1471.169 kb on - strand at 1471.193 kb on + strand at 1471.193 kb on + strand at 1471.194 kb on - strand at 1471.285 kb on - strand at 1471.286 kb on + strand at 1471.291 kb on - strand, within BT1182 at 1471.368 kb on - strand, within BT1182 at 1471.371 kb on - strand, within BT1182 at 1471.401 kb on - strand, within BT1182 at 1471.421 kb on - strand, within BT1182 at 1471.592 kb on + strand, within BT1182 at 1471.760 kb on + strand, within BT1182 at 1471.820 kb on - strand, within BT1182 at 1471.836 kb on - strand, within BT1182 at 1471.847 kb on - strand, within BT1182 at 1471.884 kb on - strand, within BT1182 at 1471.884 kb on - strand, within BT1182 at 1471.940 kb on + strand, within BT1182 at 1472.015 kb on - strand, within BT1182 at 1472.103 kb on - strand, within BT1182 at 1472.103 kb on - strand, within BT1182
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,469,279 + BT1180 0.16 -1.2 1,469,280 - BT1180 0.16 +0.8 1,469,319 - BT1180 0.19 +2.1 1,469,323 - BT1180 0.19 -0.8 1,469,403 + BT1180 0.26 -1.8 1,469,607 + BT1180 0.44 -1.2 1,469,608 - BT1180 0.44 -1.4 1,469,608 - BT1180 0.44 -0.3 1,469,701 - BT1180 0.52 -2.5 1,469,713 - BT1180 0.53 -0.5 1,469,719 - BT1180 0.54 -1.2 1,469,762 + BT1180 0.57 -0.4 1,469,819 - BT1180 0.62 +2.1 1,470,003 + BT1180 0.78 -0.8 1,470,004 - BT1180 0.78 -1.6 1,470,004 - BT1180 0.78 -0.6 1,470,051 - BT1180 0.83 +0.8 1,470,264 - -0.8 1,470,395 - BT1181 0.15 -3.3 1,470,395 - BT1181 0.15 -1.3 1,470,434 + BT1181 0.20 -1.2 1,470,435 - BT1181 0.20 +2.0 1,470,544 - BT1181 0.33 -1.2 1,470,643 - BT1181 0.44 -2.2 1,470,680 - BT1181 0.49 -1.5 1,470,779 + BT1181 0.60 -0.2 1,470,782 - BT1181 0.61 +3.3 1,470,830 + BT1181 0.66 -2.2 1,470,849 - BT1181 0.69 -3.0 1,470,884 + BT1181 0.73 +0.8 1,470,921 - BT1181 0.77 -2.7 1,470,980 + BT1181 0.84 -2.8 1,470,981 - BT1181 0.84 -0.2 1,471,017 - BT1181 0.88 -0.5 1,471,022 - BT1181 0.89 -2.7 1,471,138 - +2.7 1,471,149 + -0.2 1,471,152 - -1.2 1,471,157 - -0.2 1,471,169 - +1.0 1,471,193 + -0.2 1,471,193 + -1.8 1,471,194 - +0.3 1,471,285 - -1.5 1,471,286 + +2.1 1,471,291 - BT1182 0.10 -1.8 1,471,368 - BT1182 0.15 +2.5 1,471,371 - BT1182 0.15 -1.2 1,471,401 - BT1182 0.17 -2.9 1,471,421 - BT1182 0.19 -0.2 1,471,592 + BT1182 0.30 -1.8 1,471,760 + BT1182 0.41 +3.3 1,471,820 - BT1182 0.45 +2.3 1,471,836 - BT1182 0.46 +2.1 1,471,847 - BT1182 0.47 +0.4 1,471,884 - BT1182 0.50 -0.5 1,471,884 - BT1182 0.50 -1.5 1,471,940 + BT1182 0.53 -3.0 1,472,015 - BT1182 0.58 +0.2 1,472,103 - BT1182 0.64 -1.9 1,472,103 - BT1182 0.64 -1.8
Or see this region's nucleotide sequence