Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1091 and BT1092 are separated by 63 nucleotides BT1092 and BT1093 are separated by 23 nucleotides BT1093 and BT1094 are separated by 291 nucleotides BT1094 and BT1095 are separated by 413 nucleotides
BT1091: BT1091 - cation-transporting ATPase pacS (NCBI ptt file), at 1,378,180 to 1,380,396
BT1091
BT1092: BT1092 - putative heavy-metal binding protein (NCBI ptt file), at 1,380,460 to 1,380,819
BT1092
BT1093: BT1093 - conserved hypothetical protein, putative TonB-dependent outer membrane receptor protein (NCBI ptt file), at 1,380,843 to 1,381,946
BT1093
BT1094: BT1094 - hypothetical protein (NCBI ptt file), at 1,382,238 to 1,382,474
BT1094
BT1095: BT1095 - conserved hypothetical protein (NCBI ptt file), at 1,382,888 to 1,383,199
BT1095
Position (kb)
1380
1381
1382 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1379.940 kb on + strand, within BT1091 at 1380.015 kb on + strand, within BT1091 at 1380.058 kb on + strand, within BT1091 at 1380.059 kb on - strand, within BT1091 at 1380.075 kb on + strand, within BT1091 at 1380.094 kb on + strand, within BT1091 at 1380.131 kb on + strand, within BT1091 at 1380.267 kb on + strand at 1380.357 kb on + strand at 1380.358 kb on - strand at 1380.361 kb on - strand at 1380.386 kb on - strand at 1380.388 kb on - strand at 1380.461 kb on + strand at 1380.492 kb on + strand at 1380.841 kb on + strand at 1381.080 kb on + strand, within BT1093 at 1381.240 kb on + strand, within BT1093 at 1381.314 kb on + strand, within BT1093 at 1381.333 kb on + strand, within BT1093 at 1381.334 kb on - strand, within BT1093 at 1381.362 kb on + strand, within BT1093 at 1381.455 kb on + strand, within BT1093 at 1381.461 kb on + strand, within BT1093 at 1381.465 kb on + strand, within BT1093 at 1381.633 kb on + strand, within BT1093 at 1381.634 kb on - strand, within BT1093 at 1381.637 kb on + strand, within BT1093 at 1381.679 kb on + strand, within BT1093 at 1381.903 kb on + strand at 1381.904 kb on - strand at 1381.920 kb on + strand at 1381.920 kb on + strand at 1381.921 kb on - strand at 1381.921 kb on - strand at 1381.929 kb on + strand at 1381.995 kb on - strand at 1381.995 kb on - strand at 1382.009 kb on + strand at 1382.277 kb on + strand, within BT1094 at 1382.311 kb on - strand, within BT1094 at 1382.327 kb on - strand, within BT1094 at 1382.363 kb on + strand, within BT1094 at 1382.390 kb on - strand, within BT1094 at 1382.462 kb on + strand at 1382.700 kb on - strand at 1382.700 kb on - strand at 1382.921 kb on - strand, within BT1095 at 1382.923 kb on - strand, within BT1095 at 1382.923 kb on - strand, within BT1095
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,379,940 + BT1091 0.79 -1.0 1,380,015 + BT1091 0.83 -0.0 1,380,058 + BT1091 0.85 -0.3 1,380,059 - BT1091 0.85 -2.0 1,380,075 + BT1091 0.85 -0.3 1,380,094 + BT1091 0.86 -1.3 1,380,131 + BT1091 0.88 -3.0 1,380,267 + -1.9 1,380,357 + -1.9 1,380,358 - -2.6 1,380,361 - -0.9 1,380,386 - -0.3 1,380,388 - -0.5 1,380,461 + +0.6 1,380,492 + -0.3 1,380,841 + +0.3 1,381,080 + BT1093 0.21 +1.4 1,381,240 + BT1093 0.36 +0.7 1,381,314 + BT1093 0.43 +0.4 1,381,333 + BT1093 0.44 +0.5 1,381,334 - BT1093 0.44 +0.3 1,381,362 + BT1093 0.47 +2.1 1,381,455 + BT1093 0.55 +0.7 1,381,461 + BT1093 0.56 -2.6 1,381,465 + BT1093 0.56 -1.3 1,381,633 + BT1093 0.72 -0.6 1,381,634 - BT1093 0.72 +3.3 1,381,637 + BT1093 0.72 +3.5 1,381,679 + BT1093 0.76 -1.9 1,381,903 + +0.7 1,381,904 - -1.3 1,381,920 + +2.5 1,381,920 + -0.3 1,381,921 - -1.0 1,381,921 - -2.3 1,381,929 + +0.1 1,381,995 - -2.1 1,381,995 - -0.3 1,382,009 + -1.9 1,382,277 + BT1094 0.16 -1.1 1,382,311 - BT1094 0.31 -0.0 1,382,327 - BT1094 0.38 -2.3 1,382,363 + BT1094 0.53 +1.3 1,382,390 - BT1094 0.64 -2.3 1,382,462 + +1.3 1,382,700 - +1.1 1,382,700 - +1.7 1,382,921 - BT1095 0.11 +1.3 1,382,923 - BT1095 0.11 -0.3 1,382,923 - BT1095 0.11 -1.3
Or see this region's nucleotide sequence