Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1062 and BT1063 are separated by 219 nucleotides BT1063 and BT1064 are separated by 36 nucleotides
BT1062: BT1062 - hypothetical protein (NCBI ptt file), at 1,349,217 to 1,350,170
BT1062
BT1063: BT1063 - hypothetical protein (NCBI ptt file), at 1,350,390 to 1,352,090
BT1063
BT1064: BT1064 - conserved hypothetical protein (NCBI ptt file), at 1,352,127 to 1,353,305
BT1064
Position (kb)
1350
1351
1352
1353 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1349.392 kb on - strand, within BT1062 at 1349.468 kb on + strand, within BT1062 at 1349.512 kb on + strand, within BT1062 at 1349.512 kb on + strand, within BT1062 at 1349.512 kb on + strand, within BT1062 at 1349.515 kb on + strand, within BT1062 at 1349.515 kb on + strand, within BT1062 at 1349.735 kb on - strand, within BT1062 at 1349.959 kb on + strand, within BT1062 at 1350.106 kb on - strand at 1350.146 kb on - strand at 1350.150 kb on + strand at 1350.151 kb on - strand at 1350.151 kb on - strand at 1350.151 kb on - strand at 1350.152 kb on + strand at 1350.192 kb on - strand at 1350.193 kb on + strand at 1350.196 kb on - strand at 1350.249 kb on + strand at 1350.250 kb on - strand at 1350.250 kb on - strand at 1350.272 kb on + strand at 1350.374 kb on + strand at 1350.377 kb on + strand at 1350.428 kb on + strand at 1350.434 kb on + strand at 1350.542 kb on - strand at 1350.596 kb on + strand, within BT1063 at 1350.631 kb on - strand, within BT1063 at 1350.640 kb on - strand, within BT1063 at 1350.644 kb on - strand, within BT1063 at 1350.668 kb on + strand, within BT1063 at 1350.696 kb on + strand, within BT1063 at 1350.696 kb on + strand, within BT1063 at 1350.771 kb on - strand, within BT1063 at 1350.796 kb on - strand, within BT1063 at 1350.804 kb on - strand, within BT1063 at 1350.815 kb on + strand, within BT1063 at 1350.891 kb on + strand, within BT1063 at 1350.924 kb on + strand, within BT1063 at 1351.003 kb on + strand, within BT1063 at 1351.036 kb on - strand, within BT1063 at 1351.044 kb on - strand, within BT1063 at 1351.048 kb on + strand, within BT1063 at 1351.049 kb on - strand, within BT1063 at 1351.049 kb on - strand, within BT1063 at 1351.062 kb on - strand, within BT1063 at 1351.079 kb on + strand, within BT1063 at 1351.121 kb on + strand, within BT1063 at 1351.151 kb on + strand, within BT1063 at 1351.158 kb on - strand, within BT1063 at 1351.158 kb on - strand, within BT1063 at 1351.158 kb on - strand, within BT1063 at 1351.159 kb on + strand, within BT1063 at 1351.159 kb on + strand, within BT1063 at 1351.160 kb on - strand, within BT1063 at 1351.193 kb on - strand, within BT1063 at 1351.196 kb on + strand, within BT1063 at 1351.223 kb on + strand, within BT1063 at 1351.242 kb on - strand, within BT1063 at 1351.244 kb on + strand, within BT1063 at 1351.244 kb on + strand, within BT1063 at 1351.263 kb on + strand, within BT1063 at 1351.308 kb on - strand, within BT1063 at 1351.334 kb on + strand, within BT1063 at 1351.365 kb on - strand, within BT1063 at 1351.396 kb on + strand, within BT1063 at 1351.438 kb on - strand, within BT1063 at 1351.451 kb on + strand, within BT1063 at 1351.551 kb on - strand, within BT1063 at 1351.559 kb on + strand, within BT1063 at 1351.581 kb on - strand, within BT1063 at 1351.760 kb on + strand, within BT1063 at 1351.948 kb on - strand at 1352.065 kb on - strand at 1352.083 kb on + strand at 1352.283 kb on + strand, within BT1064 at 1352.473 kb on + strand, within BT1064 at 1352.624 kb on + strand, within BT1064 at 1352.626 kb on + strand, within BT1064 at 1352.626 kb on + strand, within BT1064 at 1352.905 kb on - strand, within BT1064 at 1352.934 kb on - strand, within BT1064 at 1353.054 kb on + strand, within BT1064
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,349,392 - BT1062 0.18 -0.3 1,349,468 + BT1062 0.26 +0.5 1,349,512 + BT1062 0.31 +1.3 1,349,512 + BT1062 0.31 -1.6 1,349,512 + BT1062 0.31 -1.8 1,349,515 + BT1062 0.31 -1.3 1,349,515 + BT1062 0.31 -1.3 1,349,735 - BT1062 0.54 -3.4 1,349,959 + BT1062 0.78 -0.9 1,350,106 - -0.3 1,350,146 - -3.6 1,350,150 + -1.9 1,350,151 - -2.6 1,350,151 - -1.9 1,350,151 - +0.8 1,350,152 + +0.3 1,350,192 - -1.3 1,350,193 + -0.5 1,350,196 - -1.6 1,350,249 + +0.1 1,350,250 - -1.5 1,350,250 - +2.1 1,350,272 + -1.9 1,350,374 + -0.1 1,350,377 + +1.3 1,350,428 + -1.3 1,350,434 + +1.7 1,350,542 - -2.3 1,350,596 + BT1063 0.12 -2.1 1,350,631 - BT1063 0.14 +1.7 1,350,640 - BT1063 0.15 +1.1 1,350,644 - BT1063 0.15 +2.1 1,350,668 + BT1063 0.16 +1.3 1,350,696 + BT1063 0.18 +0.3 1,350,696 + BT1063 0.18 -1.3 1,350,771 - BT1063 0.22 -0.3 1,350,796 - BT1063 0.24 -4.1 1,350,804 - BT1063 0.24 +0.4 1,350,815 + BT1063 0.25 +0.3 1,350,891 + BT1063 0.29 +1.3 1,350,924 + BT1063 0.31 +1.1 1,351,003 + BT1063 0.36 -2.6 1,351,036 - BT1063 0.38 -0.3 1,351,044 - BT1063 0.38 -1.9 1,351,048 + BT1063 0.39 -0.5 1,351,049 - BT1063 0.39 +1.2 1,351,049 - BT1063 0.39 +0.1 1,351,062 - BT1063 0.40 -1.3 1,351,079 + BT1063 0.41 -0.1 1,351,121 + BT1063 0.43 +1.7 1,351,151 + BT1063 0.45 +2.0 1,351,158 - BT1063 0.45 +0.5 1,351,158 - BT1063 0.45 +1.7 1,351,158 - BT1063 0.45 +1.3 1,351,159 + BT1063 0.45 -1.6 1,351,159 + BT1063 0.45 -0.9 1,351,160 - BT1063 0.45 +1.5 1,351,193 - BT1063 0.47 +2.0 1,351,196 + BT1063 0.47 -0.4 1,351,223 + BT1063 0.49 -1.9 1,351,242 - BT1063 0.50 -3.5 1,351,244 + BT1063 0.50 -1.9 1,351,244 + BT1063 0.50 -0.3 1,351,263 + BT1063 0.51 -0.5 1,351,308 - BT1063 0.54 -2.5 1,351,334 + BT1063 0.55 +1.9 1,351,365 - BT1063 0.57 +0.1 1,351,396 + BT1063 0.59 +0.8 1,351,438 - BT1063 0.62 -2.3 1,351,451 + BT1063 0.62 -0.3 1,351,551 - BT1063 0.68 -1.2 1,351,559 + BT1063 0.69 -2.4 1,351,581 - BT1063 0.70 +0.7 1,351,760 + BT1063 0.81 -1.3 1,351,948 - +2.0 1,352,065 - +1.0 1,352,083 + +0.7 1,352,283 + BT1064 0.13 +2.0 1,352,473 + BT1064 0.29 -1.3 1,352,624 + BT1064 0.42 -2.6 1,352,626 + BT1064 0.42 -0.3 1,352,626 + BT1064 0.42 +0.1 1,352,905 - BT1064 0.66 -0.3 1,352,934 - BT1064 0.68 +0.7 1,353,054 + BT1064 0.79 +1.4
Or see this region's nucleotide sequence