Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0009 and BT0010 are separated by 54 nucleotides BT0010 and BT0011 are separated by 205 nucleotides
BT0009: BT0009 - phosphate-binding periplasmic protein precursor, putative ABC transporter (NCBI ptt file), at 5,632 to 6,741
BT0009
BT0010: BT0010 - hypothetical protein (NCBI ptt file), at 6,796 to 7,869
BT0010
BT0011: BT0011 - hypothetical protein (NCBI ptt file), at 8,075 to 8,923
BT0011
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5.868 kb on + strand, within BT0009 at 5.925 kb on - strand, within BT0009 at 5.925 kb on - strand, within BT0009 at 5.989 kb on - strand, within BT0009 at 6.001 kb on - strand, within BT0009 at 6.086 kb on - strand, within BT0009 at 6.086 kb on - strand, within BT0009 at 6.086 kb on - strand, within BT0009 at 6.218 kb on - strand, within BT0009 at 6.516 kb on + strand, within BT0009 at 6.540 kb on - strand, within BT0009 at 6.545 kb on - strand, within BT0009 at 6.546 kb on + strand, within BT0009 at 6.716 kb on - strand at 6.716 kb on - strand at 6.744 kb on + strand at 6.750 kb on + strand at 6.751 kb on - strand at 6.758 kb on + strand at 6.759 kb on - strand at 6.834 kb on + strand at 6.858 kb on - strand at 6.858 kb on - strand at 6.925 kb on - strand, within BT0010 at 7.277 kb on + strand, within BT0010 at 7.277 kb on + strand, within BT0010 at 7.348 kb on - strand, within BT0010 at 7.348 kb on - strand, within BT0010 at 7.353 kb on - strand, within BT0010 at 7.614 kb on - strand, within BT0010 at 7.627 kb on - strand, within BT0010 at 7.756 kb on + strand, within BT0010 at 7.908 kb on + strand at 7.909 kb on - strand at 7.937 kb on - strand at 7.967 kb on - strand at 7.996 kb on + strand at 7.997 kb on - strand at 8.059 kb on - strand at 8.097 kb on - strand at 8.138 kb on - strand at 8.138 kb on - strand at 8.154 kb on - strand at 8.393 kb on + strand, within BT0011 at 8.393 kb on + strand, within BT0011 at 8.394 kb on - strand, within BT0011 at 8.448 kb on - strand, within BT0011 at 8.448 kb on - strand, within BT0011 at 8.472 kb on - strand, within BT0011 at 8.497 kb on - strand, within BT0011 at 8.505 kb on - strand, within BT0011 at 8.505 kb on - strand, within BT0011 at 8.550 kb on - strand, within BT0011 at 8.550 kb on - strand, within BT0011 at 8.550 kb on - strand, within BT0011 at 8.551 kb on + strand, within BT0011 at 8.681 kb on + strand, within BT0011 at 8.688 kb on - strand, within BT0011 at 8.711 kb on - strand, within BT0011 at 8.767 kb on + strand, within BT0011
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 5,868 + BT0009 0.21 +0.4 5,925 - BT0009 0.26 -2.1 5,925 - BT0009 0.26 -0.1 5,989 - BT0009 0.32 +2.4 6,001 - BT0009 0.33 -3.3 6,086 - BT0009 0.41 -1.1 6,086 - BT0009 0.41 +3.0 6,086 - BT0009 0.41 -1.7 6,218 - BT0009 0.53 -0.9 6,516 + BT0009 0.80 -0.7 6,540 - BT0009 0.82 -2.6 6,545 - BT0009 0.82 -1.4 6,546 + BT0009 0.82 +0.3 6,716 - -2.0 6,716 - -0.1 6,744 + +1.5 6,750 + -1.5 6,751 - -1.8 6,758 + -1.1 6,759 - -1.9 6,834 + +1.5 6,858 - -0.1 6,858 - -1.4 6,925 - BT0010 0.12 -1.1 7,277 + BT0010 0.45 +0.9 7,277 + BT0010 0.45 -2.7 7,348 - BT0010 0.51 +2.5 7,348 - BT0010 0.51 -1.6 7,353 - BT0010 0.52 +1.5 7,614 - BT0010 0.76 -1.7 7,627 - BT0010 0.77 -2.1 7,756 + BT0010 0.89 +0.9 7,908 + -1.1 7,909 - -0.1 7,937 - -0.1 7,967 - -2.3 7,996 + +2.2 7,997 - +2.1 8,059 - +1.2 8,097 - -2.0 8,138 - +0.5 8,138 - +1.5 8,154 - -3.0 8,393 + BT0011 0.37 -2.4 8,393 + BT0011 0.37 -0.1 8,394 - BT0011 0.38 -1.7 8,448 - BT0011 0.44 +2.5 8,448 - BT0011 0.44 -2.3 8,472 - BT0011 0.47 -2.8 8,497 - BT0011 0.50 -2.1 8,505 - BT0011 0.51 -1.7 8,505 - BT0011 0.51 -1.3 8,550 - BT0011 0.56 -2.7 8,550 - BT0011 0.56 -2.3 8,550 - BT0011 0.56 -1.1 8,551 + BT0011 0.56 +1.9 8,681 + BT0011 0.71 -2.1 8,688 - BT0011 0.72 -1.1 8,711 - BT0011 0.75 +0.9 8,767 + BT0011 0.82 -0.1
Or see this region's nucleotide sequence