Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_653 and Pf6N2E2_654 are separated by 532 nucleotides Pf6N2E2_654 and Pf6N2E2_655 are separated by 214 nucleotides
Pf6N2E2_653 - General secretion pathway protein F, at 743,351 to 744,562
_653
Pf6N2E2_654 - Phosphate-binding DING protein (related to PstS), at 745,095 to 746,279
_654
Pf6N2E2_655 - Filamentous haemagglutinin family outer membrane protein associated with VreARI signalling system, at 746,494 to 759,111
_655
Position (kb) 745
746
747 Strain fitness (log2 ratio) -1
0
1 at 744.608 kb on + strand at 744.645 kb on + strand at 744.646 kb on - strand at 744.646 kb on - strand at 744.646 kb on - strand at 744.817 kb on + strand at 744.818 kb on - strand at 744.818 kb on - strand at 744.964 kb on + strand at 744.964 kb on + strand at 744.964 kb on + strand at 744.965 kb on - strand at 744.965 kb on - strand at 744.965 kb on - strand at 744.965 kb on - strand at 745.206 kb on + strand at 745.245 kb on + strand, within Pf6N2E2_654 at 745.245 kb on + strand, within Pf6N2E2_654 at 745.245 kb on + strand, within Pf6N2E2_654 at 745.296 kb on + strand, within Pf6N2E2_654 at 745.297 kb on - strand, within Pf6N2E2_654 at 745.386 kb on + strand, within Pf6N2E2_654 at 745.386 kb on + strand, within Pf6N2E2_654 at 745.386 kb on + strand, within Pf6N2E2_654 at 745.386 kb on + strand, within Pf6N2E2_654 at 745.386 kb on + strand, within Pf6N2E2_654 at 745.387 kb on - strand, within Pf6N2E2_654 at 745.387 kb on - strand, within Pf6N2E2_654 at 745.387 kb on - strand, within Pf6N2E2_654 at 745.387 kb on - strand, within Pf6N2E2_654 at 745.776 kb on - strand, within Pf6N2E2_654 at 745.782 kb on + strand, within Pf6N2E2_654 at 745.782 kb on + strand, within Pf6N2E2_654 at 745.782 kb on + strand, within Pf6N2E2_654 at 745.782 kb on + strand, within Pf6N2E2_654 at 745.783 kb on - strand, within Pf6N2E2_654 at 745.783 kb on - strand, within Pf6N2E2_654 at 745.783 kb on - strand, within Pf6N2E2_654 at 745.783 kb on - strand, within Pf6N2E2_654 at 745.784 kb on + strand, within Pf6N2E2_654 at 745.784 kb on + strand, within Pf6N2E2_654 at 745.784 kb on + strand, within Pf6N2E2_654 at 745.805 kb on + strand, within Pf6N2E2_654 at 745.806 kb on - strand, within Pf6N2E2_654 at 745.889 kb on + strand, within Pf6N2E2_654 at 745.890 kb on - strand, within Pf6N2E2_654 at 745.949 kb on + strand, within Pf6N2E2_654 at 745.998 kb on - strand, within Pf6N2E2_654 at 745.998 kb on - strand, within Pf6N2E2_654 at 746.278 kb on - strand at 746.278 kb on - strand at 746.423 kb on + strand at 746.446 kb on + strand at 746.467 kb on + strand at 746.490 kb on + strand at 746.490 kb on + strand at 746.491 kb on - strand at 747.254 kb on - strand at 747.254 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 744,608 + -0.5 744,645 + -0.5 744,646 - +0.9 744,646 - -0.2 744,646 - +0.8 744,817 + -1.2 744,818 - -0.1 744,818 - +0.1 744,964 + -0.5 744,964 + -0.1 744,964 + +0.1 744,965 - -1.0 744,965 - +0.8 744,965 - -0.0 744,965 - -0.0 745,206 + +0.1 745,245 + Pf6N2E2_654 0.13 -0.5 745,245 + Pf6N2E2_654 0.13 -1.5 745,245 + Pf6N2E2_654 0.13 -0.1 745,296 + Pf6N2E2_654 0.17 +0.2 745,297 - Pf6N2E2_654 0.17 -0.6 745,386 + Pf6N2E2_654 0.25 +0.1 745,386 + Pf6N2E2_654 0.25 +0.7 745,386 + Pf6N2E2_654 0.25 +0.2 745,386 + Pf6N2E2_654 0.25 -0.7 745,386 + Pf6N2E2_654 0.25 +0.3 745,387 - Pf6N2E2_654 0.25 -1.0 745,387 - Pf6N2E2_654 0.25 +0.2 745,387 - Pf6N2E2_654 0.25 +0.5 745,387 - Pf6N2E2_654 0.25 -0.1 745,776 - Pf6N2E2_654 0.57 -0.7 745,782 + Pf6N2E2_654 0.58 -0.4 745,782 + Pf6N2E2_654 0.58 -1.0 745,782 + Pf6N2E2_654 0.58 +1.3 745,782 + Pf6N2E2_654 0.58 -1.5 745,783 - Pf6N2E2_654 0.58 -0.6 745,783 - Pf6N2E2_654 0.58 -0.5 745,783 - Pf6N2E2_654 0.58 -0.8 745,783 - Pf6N2E2_654 0.58 +0.6 745,784 + Pf6N2E2_654 0.58 +1.0 745,784 + Pf6N2E2_654 0.58 -0.6 745,784 + Pf6N2E2_654 0.58 +0.0 745,805 + Pf6N2E2_654 0.60 -0.5 745,806 - Pf6N2E2_654 0.60 +0.6 745,889 + Pf6N2E2_654 0.67 -0.3 745,890 - Pf6N2E2_654 0.67 +0.1 745,949 + Pf6N2E2_654 0.72 -0.7 745,998 - Pf6N2E2_654 0.76 -1.4 745,998 - Pf6N2E2_654 0.76 +0.2 746,278 - +0.3 746,278 - -0.0 746,423 + +1.3 746,446 + -0.4 746,467 + +0.0 746,490 + -0.3 746,490 + +0.3 746,491 - -1.7 747,254 - -0.1 747,254 - +0.5
Or see this region's nucleotide sequence