Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3943 and Pf6N2E2_3944 are separated by 283 nucleotides Pf6N2E2_3944 and Pf6N2E2_3945 are separated by 68 nucleotides Pf6N2E2_3945 and Pf6N2E2_3946 are separated by 49 nucleotides
Pf6N2E2_3943 - COG3332, at 4,580,799 to 4,581,584
_3943
Pf6N2E2_3944 - Protein of unknown function DUF81, at 4,581,868 to 4,582,668
_3944
Pf6N2E2_3945 - Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-), at 4,582,737 to 4,583,546
_3945
Pf6N2E2_3946 - Thymidylate synthase (EC 2.1.1.45), at 4,583,596 to 4,584,567
_3946
Position (kb)
4581
4582
4583 Strain fitness (log2 ratio)
-1
0
1 at 4581.032 kb on + strand, within Pf6N2E2_3943 at 4581.032 kb on + strand, within Pf6N2E2_3943 at 4581.033 kb on - strand, within Pf6N2E2_3943 at 4581.136 kb on + strand, within Pf6N2E2_3943 at 4581.136 kb on + strand, within Pf6N2E2_3943 at 4581.137 kb on - strand, within Pf6N2E2_3943 at 4581.137 kb on - strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.230 kb on - strand, within Pf6N2E2_3943 at 4581.262 kb on + strand, within Pf6N2E2_3943 at 4581.263 kb on - strand, within Pf6N2E2_3943 at 4581.263 kb on - strand, within Pf6N2E2_3943 at 4581.263 kb on - strand, within Pf6N2E2_3943 at 4581.269 kb on + strand, within Pf6N2E2_3943 at 4581.270 kb on - strand, within Pf6N2E2_3943 at 4581.478 kb on + strand, within Pf6N2E2_3943 at 4581.478 kb on + strand, within Pf6N2E2_3943 at 4581.479 kb on - strand, within Pf6N2E2_3943 at 4581.540 kb on + strand at 4581.540 kb on + strand at 4581.540 kb on + strand at 4581.540 kb on + strand at 4581.541 kb on - strand at 4581.541 kb on - strand at 4581.574 kb on + strand at 4581.575 kb on - strand at 4581.575 kb on - strand at 4581.591 kb on + strand at 4581.592 kb on - strand at 4581.640 kb on - strand at 4581.647 kb on - strand at 4581.694 kb on + strand at 4581.760 kb on + strand at 4581.786 kb on - strand at 4581.858 kb on + strand at 4581.859 kb on - strand at 4581.901 kb on + strand at 4581.901 kb on + strand at 4581.902 kb on - strand at 4581.902 kb on - strand at 4582.460 kb on + strand, within Pf6N2E2_3944 at 4582.460 kb on + strand, within Pf6N2E2_3944 at 4582.506 kb on + strand, within Pf6N2E2_3944 at 4582.506 kb on + strand, within Pf6N2E2_3944 at 4582.507 kb on - strand, within Pf6N2E2_3944 at 4582.522 kb on + strand, within Pf6N2E2_3944 at 4582.522 kb on + strand, within Pf6N2E2_3944 at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.682 kb on + strand at 4582.723 kb on + strand at 4583.511 kb on + strand at 4583.511 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 4,581,032 + Pf6N2E2_3943 0.30 +0.7 4,581,032 + Pf6N2E2_3943 0.30 -1.5 4,581,033 - Pf6N2E2_3943 0.30 +1.4 4,581,136 + Pf6N2E2_3943 0.43 -0.6 4,581,136 + Pf6N2E2_3943 0.43 +0.1 4,581,137 - Pf6N2E2_3943 0.43 -0.8 4,581,137 - Pf6N2E2_3943 0.43 -0.1 4,581,229 + Pf6N2E2_3943 0.55 +1.1 4,581,229 + Pf6N2E2_3943 0.55 -1.0 4,581,229 + Pf6N2E2_3943 0.55 +0.2 4,581,229 + Pf6N2E2_3943 0.55 -0.1 4,581,230 - Pf6N2E2_3943 0.55 +0.5 4,581,262 + Pf6N2E2_3943 0.59 -0.2 4,581,263 - Pf6N2E2_3943 0.59 -0.2 4,581,263 - Pf6N2E2_3943 0.59 -0.2 4,581,263 - Pf6N2E2_3943 0.59 +1.1 4,581,269 + Pf6N2E2_3943 0.60 +0.3 4,581,270 - Pf6N2E2_3943 0.60 +0.2 4,581,478 + Pf6N2E2_3943 0.86 +0.7 4,581,478 + Pf6N2E2_3943 0.86 +1.0 4,581,479 - Pf6N2E2_3943 0.87 +0.6 4,581,540 + +0.2 4,581,540 + +0.2 4,581,540 + -1.2 4,581,540 + -0.7 4,581,541 - +0.2 4,581,541 - +0.5 4,581,574 + -0.2 4,581,575 - +0.6 4,581,575 - -0.0 4,581,591 + -0.5 4,581,592 - +0.6 4,581,640 - -0.1 4,581,647 - +0.2 4,581,694 + +0.2 4,581,760 + +0.5 4,581,786 - +0.8 4,581,858 + -0.6 4,581,859 - +0.4 4,581,901 + -0.4 4,581,901 + -0.4 4,581,902 - +1.0 4,581,902 - +0.4 4,582,460 + Pf6N2E2_3944 0.74 -0.1 4,582,460 + Pf6N2E2_3944 0.74 -1.5 4,582,506 + Pf6N2E2_3944 0.80 +0.1 4,582,506 + Pf6N2E2_3944 0.80 -0.7 4,582,507 - Pf6N2E2_3944 0.80 -0.4 4,582,522 + Pf6N2E2_3944 0.82 +0.1 4,582,522 + Pf6N2E2_3944 0.82 -0.4 4,582,637 + +1.6 4,582,637 + +0.2 4,582,637 + +0.4 4,582,637 + +0.2 4,582,637 + +0.1 4,582,682 + +0.5 4,582,723 + -0.6 4,583,511 + -0.1 4,583,511 + +0.8
Or see this region's nucleotide sequence