Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23135 and BWI76_RS23140 are separated by 19 nucleotides BWI76_RS23140 and BWI76_RS23145 are separated by 24 nucleotides BWI76_RS23145 and BWI76_RS23150 overlap by 1 nucleotides BWI76_RS23150 and BWI76_RS23155 overlap by 4 nucleotides BWI76_RS23155 and BWI76_RS23160 are separated by 3 nucleotides BWI76_RS23160 and BWI76_RS23165 are separated by 9 nucleotides
BWI76_RS23135: BWI76_RS23135 - propanediol dehydratase, at 4,823,419 to 4,823,769
_RS23135
BWI76_RS23140: BWI76_RS23140 - carboxysome shell protein, at 4,823,789 to 4,824,064
_RS23140
BWI76_RS23145: BWI76_RS23145 - propanediol utilization protein, at 4,824,089 to 4,824,550
_RS23145
BWI76_RS23150: BWI76_RS23150 - propanediol utilization phosphotransacylase, at 4,824,550 to 4,825,182
_RS23150
BWI76_RS23155: BWI76_RS23155 - microcompartment protein PduM, at 4,825,179 to 4,825,670
_RS23155
BWI76_RS23160: BWI76_RS23160 - ethanolamine utilization protein EutN, at 4,825,674 to 4,825,949
_RS23160
BWI76_RS23165: BWI76_RS23165 - ATP:cob(I)alamin adenosyltransferase, at 4,825,959 to 4,826,969
_RS23165
Position (kb)
4824
4825
4826 Strain fitness (log2 ratio)
-2
-1
0
1 at 4823.559 kb on + strand, within BWI76_RS23135 at 4823.567 kb on - strand, within BWI76_RS23135 at 4823.914 kb on - strand, within BWI76_RS23140 at 4823.914 kb on - strand, within BWI76_RS23140 at 4824.082 kb on + strand at 4824.675 kb on - strand, within BWI76_RS23150 at 4824.841 kb on + strand, within BWI76_RS23150 at 4824.879 kb on + strand, within BWI76_RS23150 at 4825.221 kb on - strand at 4825.274 kb on + strand, within BWI76_RS23155 at 4825.417 kb on - strand, within BWI76_RS23155 at 4825.496 kb on - strand, within BWI76_RS23155 at 4825.572 kb on + strand, within BWI76_RS23155 at 4825.612 kb on + strand, within BWI76_RS23155 at 4825.973 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM remove 4,823,559 + BWI76_RS23135 0.40 -0.6 4,823,567 - BWI76_RS23135 0.42 +0.6 4,823,914 - BWI76_RS23140 0.45 -0.9 4,823,914 - BWI76_RS23140 0.45 -0.6 4,824,082 + -1.4 4,824,675 - BWI76_RS23150 0.20 -1.8 4,824,841 + BWI76_RS23150 0.46 -0.1 4,824,879 + BWI76_RS23150 0.52 -2.5 4,825,221 - -0.2 4,825,274 + BWI76_RS23155 0.19 -0.7 4,825,417 - BWI76_RS23155 0.48 +0.3 4,825,496 - BWI76_RS23155 0.64 -0.4 4,825,572 + BWI76_RS23155 0.80 +0.2 4,825,612 + BWI76_RS23155 0.88 -1.0 4,825,973 - +1.0
Or see this region's nucleotide sequence