Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19805

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19795 and BWI76_RS19800 are separated by 3 nucleotidesBWI76_RS19800 and BWI76_RS19805 are separated by 330 nucleotidesBWI76_RS19805 and BWI76_RS19810 overlap by 4 nucleotidesBWI76_RS19810 and BWI76_RS19815 are separated by 147 nucleotides BWI76_RS19795: BWI76_RS19795 - microcin C ABC transporter ATP-binding protein YejF, at 4,139,624 to 4,141,213 _RS19795 BWI76_RS19800: BWI76_RS19800 - hypothetical protein, at 4,141,217 to 4,141,561 _RS19800 BWI76_RS19805: BWI76_RS19805 - Bcr/CflA family multidrug efflux MFS transporter, at 4,141,892 to 4,143,088 _RS19805 BWI76_RS19810: BWI76_RS19810 - 16S rRNA pseudouridine(516) synthase, at 4,143,085 to 4,143,804 _RS19810 BWI76_RS19815: BWI76_RS19815 - ATP-dependent helicase, at 4,143,952 to 4,145,709 _RS19815 Position (kb) 4141 4142 4143 4144Strain fitness (log2 ratio) -3 -2 -1 0 1at 4140.926 kb on + strand, within BWI76_RS19795at 4141.032 kb on - strand, within BWI76_RS19795at 4141.052 kb on - strand, within BWI76_RS19795at 4141.109 kb on - strandat 4141.152 kb on - strandat 4141.532 kb on + strandat 4142.439 kb on - strandat 4142.620 kb on - strand, within BWI76_RS19805at 4142.877 kb on - strand, within BWI76_RS19805at 4143.089 kb on + strandat 4143.760 kb on - strandat 4143.956 kb on + strandat 4143.964 kb on - strandat 4143.964 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
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4,140,926 + BWI76_RS19795 0.82 +0.0
4,141,032 - BWI76_RS19795 0.89 -0.7
4,141,052 - BWI76_RS19795 0.90 -0.6
4,141,109 - +1.1
4,141,152 - +0.2
4,141,532 + -0.5
4,142,439 - -0.3
4,142,620 - BWI76_RS19805 0.61 -3.0
4,142,877 - BWI76_RS19805 0.82 -1.7
4,143,089 + -0.7
4,143,760 - -1.8
4,143,956 + -0.1
4,143,964 - +0.1
4,143,964 - -0.1

Or see this region's nucleotide sequence