Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16860

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16850 and BWI76_RS16855 overlap by 4 nucleotidesBWI76_RS16855 and BWI76_RS16860 are separated by 11 nucleotidesBWI76_RS16860 and BWI76_RS16865 are separated by 53 nucleotides BWI76_RS16850: BWI76_RS16850 - ABC transporter periplasmic substrate-binding protein, at 3,494,332 to 3,495,369 _RS16850 BWI76_RS16855: BWI76_RS16855 - putative ABC transporter periplasmic solute-binding protein, at 3,495,366 to 3,496,340 _RS16855 BWI76_RS16860: BWI76_RS16860 - acyl-CoA dehydrogenase, at 3,496,352 to 3,497,440 _RS16860 BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579 _RS16865 Position (kb) 3496 3497 3498Strain fitness (log2 ratio) -1 0 1at 3495.367 kb on - strandat 3495.387 kb on + strandat 3495.787 kb on + strand, within BWI76_RS16855at 3496.282 kb on - strandat 3496.429 kb on - strandat 3496.666 kb on + strand, within BWI76_RS16860at 3496.720 kb on - strand, within BWI76_RS16860at 3497.655 kb on + strand, within BWI76_RS16865at 3497.663 kb on + strand, within BWI76_RS16865at 3497.663 kb on - strand, within BWI76_RS16865at 3497.738 kb on + strand, within BWI76_RS16865at 3498.029 kb on + strand, within BWI76_RS16865at 3498.154 kb on + strand, within BWI76_RS16865at 3498.173 kb on + strandat 3498.181 kb on - strand, within BWI76_RS16865

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
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3,495,367 - +0.4
3,495,387 + +0.7
3,495,787 + BWI76_RS16855 0.43 -0.1
3,496,282 - +0.6
3,496,429 - -1.1
3,496,666 + BWI76_RS16860 0.29 +0.6
3,496,720 - BWI76_RS16860 0.34 +0.9
3,497,655 + BWI76_RS16865 0.15 +0.8
3,497,663 + BWI76_RS16865 0.16 +0.4
3,497,663 - BWI76_RS16865 0.16 +0.6
3,497,738 + BWI76_RS16865 0.22 +0.2
3,498,029 + BWI76_RS16865 0.49 -0.2
3,498,154 + BWI76_RS16865 0.61 +0.3
3,498,173 + -0.8
3,498,181 - BWI76_RS16865 0.63 +1.5

Or see this region's nucleotide sequence