Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12860

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS12850 and BWI76_RS12855 are separated by 23 nucleotidesBWI76_RS12855 and BWI76_RS12860 overlap by 4 nucleotidesBWI76_RS12860 and BWI76_RS12865 overlap by 8 nucleotides BWI76_RS12850: BWI76_RS12850 - hypothetical protein, at 2,678,472 to 2,679,731 _RS12850 BWI76_RS12855: BWI76_RS12855 - enamine deaminase RidA, at 2,679,755 to 2,680,189 _RS12855 BWI76_RS12860: BWI76_RS12860 - hypothetical protein, at 2,680,186 to 2,680,860 _RS12860 BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022 _RS12865 Position (kb) 2680 2681Strain fitness (log2 ratio) -2 -1 0 1at 2679.228 kb on - strand, within BWI76_RS12850at 2679.241 kb on + strand, within BWI76_RS12850at 2679.249 kb on - strand, within BWI76_RS12850at 2679.347 kb on + strand, within BWI76_RS12850at 2679.389 kb on - strand, within BWI76_RS12850at 2679.769 kb on - strandat 2679.837 kb on - strand, within BWI76_RS12855at 2680.044 kb on + strand, within BWI76_RS12855at 2680.303 kb on + strand, within BWI76_RS12860at 2680.518 kb on - strand, within BWI76_RS12860at 2681.019 kb on - strand, within BWI76_RS12865at 2681.110 kb on - strand, within BWI76_RS12865at 2681.268 kb on + strand, within BWI76_RS12865at 2681.368 kb on - strand, within BWI76_RS12865at 2681.368 kb on - strand, within BWI76_RS12865at 2681.518 kb on - strand, within BWI76_RS12865

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
remove
2,679,228 - BWI76_RS12850 0.60 -1.4
2,679,241 + BWI76_RS12850 0.61 -0.5
2,679,249 - BWI76_RS12850 0.62 +0.6
2,679,347 + BWI76_RS12850 0.69 +0.4
2,679,389 - BWI76_RS12850 0.73 +0.5
2,679,769 - -0.4
2,679,837 - BWI76_RS12855 0.19 -0.2
2,680,044 + BWI76_RS12855 0.66 -1.4
2,680,303 + BWI76_RS12860 0.17 -1.4
2,680,518 - BWI76_RS12860 0.49 -1.9
2,681,019 - BWI76_RS12865 0.14 -1.2
2,681,110 - BWI76_RS12865 0.22 -0.3
2,681,268 + BWI76_RS12865 0.35 -0.2
2,681,368 - BWI76_RS12865 0.44 -0.3
2,681,368 - BWI76_RS12865 0.44 -0.6
2,681,518 - BWI76_RS12865 0.57 -2.0

Or see this region's nucleotide sequence