Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10650 and BWI76_RS10655 are separated by 15 nucleotides BWI76_RS10655 and BWI76_RS10660 overlap by 35 nucleotides BWI76_RS10660 and BWI76_RS10665 are separated by 51 nucleotides BWI76_RS10665 and BWI76_RS10670 are separated by 20 nucleotides
BWI76_RS10650: BWI76_RS10650 - PTS fructose-like transporter subunit EIIB, at 2,248,865 to 2,249,206
_RS10650
BWI76_RS10655: BWI76_RS10655 - [formate-C-acetyltransferase]-activating enzyme, at 2,249,222 to 2,250,097
_RS10655
BWI76_RS10660: BWI76_RS10660 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 2,250,063 to 2,252,360
_RS10660
BWI76_RS10665: BWI76_RS10665 - PTS system fructose-like transporter subunit EIIB, at 2,252,412 to 2,252,732
_RS10665
BWI76_RS10670: BWI76_RS10670 - PTS fructose transporter subunit IIC, at 2,252,753 to 2,253,829
_RS10670
Position (kb)
2250
2251
2252
2253 Strain fitness (log2 ratio)
-2
-1
0
1 at 2249.148 kb on + strand, within BWI76_RS10650 at 2249.150 kb on + strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2250.094 kb on - strand at 2250.097 kb on + strand at 2250.243 kb on - strand at 2250.342 kb on - strand, within BWI76_RS10660 at 2250.378 kb on - strand, within BWI76_RS10660 at 2250.881 kb on - strand, within BWI76_RS10660 at 2250.931 kb on + strand, within BWI76_RS10660 at 2250.993 kb on - strand, within BWI76_RS10660 at 2251.003 kb on - strand, within BWI76_RS10660 at 2251.246 kb on - strand, within BWI76_RS10660 at 2251.384 kb on - strand, within BWI76_RS10660 at 2251.606 kb on - strand, within BWI76_RS10660 at 2251.744 kb on - strand, within BWI76_RS10660 at 2251.746 kb on + strand, within BWI76_RS10660 at 2251.766 kb on - strand, within BWI76_RS10660 at 2251.904 kb on - strand, within BWI76_RS10660 at 2251.904 kb on - strand, within BWI76_RS10660 at 2251.948 kb on + strand, within BWI76_RS10660 at 2252.107 kb on + strand, within BWI76_RS10660 at 2252.677 kb on + strand, within BWI76_RS10665 at 2252.682 kb on + strand, within BWI76_RS10665 at 2252.685 kb on - strand, within BWI76_RS10665 at 2252.790 kb on + strand at 2252.823 kb on + strand at 2252.841 kb on - strand at 2252.934 kb on + strand, within BWI76_RS10670 at 2252.934 kb on + strand, within BWI76_RS10670 at 2252.980 kb on - strand, within BWI76_RS10670 at 2253.276 kb on + strand, within BWI76_RS10670
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM remove 2,249,148 + BWI76_RS10650 0.83 +0.6 2,249,150 + BWI76_RS10650 0.83 +0.9 2,249,156 - BWI76_RS10650 0.85 +0.7 2,249,156 - BWI76_RS10650 0.85 +1.5 2,249,389 + BWI76_RS10655 0.19 -0.2 2,249,389 + BWI76_RS10655 0.19 +0.9 2,249,389 + BWI76_RS10655 0.19 -0.5 2,249,397 - BWI76_RS10655 0.20 -0.8 2,249,397 - BWI76_RS10655 0.20 -1.0 2,250,094 - +0.0 2,250,097 + +0.1 2,250,243 - +0.2 2,250,342 - BWI76_RS10660 0.12 -0.3 2,250,378 - BWI76_RS10660 0.14 -0.2 2,250,881 - BWI76_RS10660 0.36 -2.3 2,250,931 + BWI76_RS10660 0.38 -0.3 2,250,993 - BWI76_RS10660 0.40 -2.0 2,251,003 - BWI76_RS10660 0.41 -0.3 2,251,246 - BWI76_RS10660 0.51 +0.6 2,251,384 - BWI76_RS10660 0.57 -2.2 2,251,606 - BWI76_RS10660 0.67 +0.1 2,251,744 - BWI76_RS10660 0.73 -0.4 2,251,746 + BWI76_RS10660 0.73 +0.5 2,251,766 - BWI76_RS10660 0.74 -0.7 2,251,904 - BWI76_RS10660 0.80 +1.4 2,251,904 - BWI76_RS10660 0.80 -0.5 2,251,948 + BWI76_RS10660 0.82 -0.5 2,252,107 + BWI76_RS10660 0.89 +0.2 2,252,677 + BWI76_RS10665 0.83 -0.7 2,252,682 + BWI76_RS10665 0.84 -0.6 2,252,685 - BWI76_RS10665 0.85 +0.1 2,252,790 + -0.1 2,252,823 + -1.7 2,252,841 - -1.5 2,252,934 + BWI76_RS10670 0.17 +0.0 2,252,934 + BWI76_RS10670 0.17 -0.5 2,252,980 - BWI76_RS10670 0.21 -1.7 2,253,276 + BWI76_RS10670 0.49 -0.9
Or see this region's nucleotide sequence