Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07820 and BWI76_RS07825 are separated by 102 nucleotides BWI76_RS07825 and BWI76_RS07830 are separated by 46 nucleotides
BWI76_RS07820: BWI76_RS07820 - S-methyl-5-thioribose kinase, at 1,657,944 to 1,659,143
_RS07820
BWI76_RS07825: BWI76_RS07825 - S-methyl-5-thioribose-1-phosphate isomerase, at 1,659,246 to 1,660,274
_RS07825
BWI76_RS07830: BWI76_RS07830 - 3-phytase, at 1,660,321 to 1,661,589
_RS07830
Position (kb)
1659
1660
1661 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1658.800 kb on + strand, within BWI76_RS07820 at 1658.927 kb on - strand, within BWI76_RS07820 at 1659.263 kb on - strand at 1659.270 kb on - strand at 1659.276 kb on - strand at 1659.344 kb on + strand at 1659.450 kb on + strand, within BWI76_RS07825 at 1659.536 kb on - strand, within BWI76_RS07825 at 1659.831 kb on - strand, within BWI76_RS07825 at 1659.831 kb on - strand, within BWI76_RS07825 at 1659.831 kb on - strand, within BWI76_RS07825 at 1659.885 kb on - strand, within BWI76_RS07825 at 1659.920 kb on - strand, within BWI76_RS07825 at 1660.053 kb on - strand, within BWI76_RS07825 at 1660.136 kb on - strand, within BWI76_RS07825 at 1660.206 kb on + strand at 1660.236 kb on - strand at 1660.238 kb on + strand at 1660.408 kb on - strand at 1660.733 kb on + strand, within BWI76_RS07830 at 1661.173 kb on - strand, within BWI76_RS07830
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM remove 1,658,800 + BWI76_RS07820 0.71 +0.9 1,658,927 - BWI76_RS07820 0.82 +0.7 1,659,263 - -0.2 1,659,270 - -1.1 1,659,276 - -0.1 1,659,344 + +0.1 1,659,450 + BWI76_RS07825 0.20 +0.5 1,659,536 - BWI76_RS07825 0.28 +0.1 1,659,831 - BWI76_RS07825 0.57 +0.1 1,659,831 - BWI76_RS07825 0.57 +0.4 1,659,831 - BWI76_RS07825 0.57 +1.9 1,659,885 - BWI76_RS07825 0.62 +0.3 1,659,920 - BWI76_RS07825 0.66 +0.1 1,660,053 - BWI76_RS07825 0.78 +0.7 1,660,136 - BWI76_RS07825 0.86 -0.8 1,660,206 + -2.9 1,660,236 - -4.6 1,660,238 + +0.2 1,660,408 - +0.4 1,660,733 + BWI76_RS07830 0.32 +0.3 1,661,173 - BWI76_RS07830 0.67 +0.5
Or see this region's nucleotide sequence