Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05895

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05890 and BWI76_RS05895 are separated by 144 nucleotidesBWI76_RS05895 and BWI76_RS05900 overlap by 4 nucleotidesBWI76_RS05900 and BWI76_RS05905 are separated by 69 nucleotides BWI76_RS05890: BWI76_RS05890 - haloacid dehalogenase, at 1,238,111 to 1,238,773 _RS05890 BWI76_RS05895: BWI76_RS05895 - phosphonate ABC transporter, permease protein PhnE, at 1,238,918 to 1,239,799 _RS05895 BWI76_RS05900: BWI76_RS05900 - phosphonate ABC transporter, permease protein PhnE, at 1,239,796 to 1,240,668 _RS05900 BWI76_RS05905: BWI76_RS05905 - phosphonate ABC transporter substrate-binding protein, at 1,240,738 to 1,241,679 _RS05905 Position (kb) 1238 1239 1240Strain fitness (log2 ratio) -2 -1 0 1at 1238.123 kb on - strandat 1238.192 kb on - strand, within BWI76_RS05890at 1238.192 kb on - strand, within BWI76_RS05890at 1238.192 kb on - strand, within BWI76_RS05890at 1238.311 kb on + strand, within BWI76_RS05890at 1238.511 kb on + strand, within BWI76_RS05890at 1238.568 kb on + strand, within BWI76_RS05890at 1238.592 kb on + strand, within BWI76_RS05890at 1238.706 kb on + strand, within BWI76_RS05890at 1238.843 kb on - strandat 1238.903 kb on - strandat 1238.925 kb on + strandat 1239.027 kb on - strand, within BWI76_RS05895at 1239.207 kb on + strand, within BWI76_RS05895at 1239.711 kb on - strandat 1239.810 kb on + strandat 1239.908 kb on + strand, within BWI76_RS05900at 1239.974 kb on - strand, within BWI76_RS05900at 1240.080 kb on + strand, within BWI76_RS05900at 1240.162 kb on + strand, within BWI76_RS05900at 1240.225 kb on + strand, within BWI76_RS05900at 1240.267 kb on + strand, within BWI76_RS05900at 1240.336 kb on + strand, within BWI76_RS05900at 1240.679 kb on + strandat 1240.735 kb on + strandat 1240.745 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
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1,238,123 - -0.3
1,238,192 - BWI76_RS05890 0.12 +0.3
1,238,192 - BWI76_RS05890 0.12 +0.5
1,238,192 - BWI76_RS05890 0.12 -1.3
1,238,311 + BWI76_RS05890 0.30 -0.4
1,238,511 + BWI76_RS05890 0.60 -0.8
1,238,568 + BWI76_RS05890 0.69 +0.3
1,238,592 + BWI76_RS05890 0.73 -0.2
1,238,706 + BWI76_RS05890 0.90 -0.1
1,238,843 - -0.9
1,238,903 - +0.2
1,238,925 + -0.4
1,239,027 - BWI76_RS05895 0.12 -2.1
1,239,207 + BWI76_RS05895 0.33 -1.7
1,239,711 - -0.9
1,239,810 + -0.8
1,239,908 + BWI76_RS05900 0.13 +0.1
1,239,974 - BWI76_RS05900 0.20 -0.0
1,240,080 + BWI76_RS05900 0.33 +0.8
1,240,162 + BWI76_RS05900 0.42 +0.6
1,240,225 + BWI76_RS05900 0.49 -0.0
1,240,267 + BWI76_RS05900 0.54 -0.4
1,240,336 + BWI76_RS05900 0.62 -0.5
1,240,679 + -1.6
1,240,735 + +0.5
1,240,745 + -1.5

Or see this region's nucleotide sequence