Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05410

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05395 and BWI76_RS05400 are separated by 54 nucleotidesBWI76_RS05400 and BWI76_RS05405 are separated by 55 nucleotidesBWI76_RS05405 and BWI76_RS05410 are separated by 163 nucleotidesBWI76_RS05410 and BWI76_RS05415 are separated by 30 nucleotides BWI76_RS05395: BWI76_RS05395 - 23S ribosomal RNA, at 1,132,778 to 1,135,705 _RS05395 BWI76_RS05400: BWI76_RS05400 - 5S ribosomal RNA, at 1,135,760 to 1,135,875 _RS05400 BWI76_RS05405: BWI76_RS05405 - tRNA-Asp, at 1,135,931 to 1,136,007 _RS05405 BWI76_RS05410: BWI76_RS05410 - 2,5-didehydrogluconate reductase B, at 1,136,171 to 1,136,974 _RS05410 BWI76_RS05415: BWI76_RS05415 - LysR family transcriptional regulator, at 1,137,005 to 1,137,907 _RS05415 Position (kb) 1136 1137Strain fitness (log2 ratio) -2 -1 0 1at 1136.583 kb on - strand, within BWI76_RS05410at 1136.637 kb on + strand, within BWI76_RS05410at 1136.675 kb on + strand, within BWI76_RS05410at 1136.785 kb on + strand, within BWI76_RS05410at 1137.065 kb on - strandat 1137.349 kb on + strand, within BWI76_RS05415at 1137.383 kb on - strand, within BWI76_RS05415at 1137.448 kb on + strand, within BWI76_RS05415at 1137.448 kb on + strandat 1137.456 kb on - strand, within BWI76_RS05415at 1137.456 kb on - strand, within BWI76_RS05415at 1137.471 kb on - strand, within BWI76_RS05415

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
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1,136,583 - BWI76_RS05410 0.51 -2.0
1,136,637 + BWI76_RS05410 0.58 -1.9
1,136,675 + BWI76_RS05410 0.63 -1.4
1,136,785 + BWI76_RS05410 0.76 -1.3
1,137,065 - +0.0
1,137,349 + BWI76_RS05415 0.38 +0.3
1,137,383 - BWI76_RS05415 0.42 +0.2
1,137,448 + BWI76_RS05415 0.49 -0.4
1,137,448 + +0.5
1,137,456 - BWI76_RS05415 0.50 -1.2
1,137,456 - BWI76_RS05415 0.50 +0.6
1,137,471 - BWI76_RS05415 0.52 +0.4

Or see this region's nucleotide sequence